Genome insight and description of antibiotic producing Massilia antibiotica sp. nov., isolated from oil-contaminated soil

Abstract An ivory-coloured, motile, Gram-stain-negative bacterium, designated TW-1T was isolated from oil-contaminated experimental soil in Kyonggi University. The phylogenetic analysis based on 16S rRNA gene sequence revealed, strain TW-1T formed a lineage within the family Oxalobacteraceae and clu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ram Hari Dahal, Dhiraj Kumar Chaudhary, Jaisoo Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ca957062dac345298372e00fd37a0416
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ca957062dac345298372e00fd37a0416
record_format	dspace
spelling	oai:doaj.org-article:ca957062dac345298372e00fd37a04162021-12-02T13:24:15ZGenome insight and description of antibiotic producing Massilia antibiotica sp. nov., isolated from oil-contaminated soil10.1038/s41598-021-86232-z2045-2322https://doaj.org/article/ca957062dac345298372e00fd37a04162021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86232-zhttps://doaj.org/toc/2045-2322Abstract An ivory-coloured, motile, Gram-stain-negative bacterium, designated TW-1T was isolated from oil-contaminated experimental soil in Kyonggi University. The phylogenetic analysis based on 16S rRNA gene sequence revealed, strain TW-1T formed a lineage within the family Oxalobacteraceae and clustered as members of the genus Massilia. The closest members were M. pinisoli T33T (98.8% sequence similarity), M. putida 6NM-7T (98.6%), M. arvi THG-RS2OT (98.5%), M. phosphatilytica 12-OD1T (98.3%) and M. niastensis 5516S-1T (98.2%). The sole respiratory quinone is ubiquinone-8. The major cellular fatty acids are hexadeconic acid, cis-9, methylenehexadeconic acid, summed feature 3 and summed feature 8. The major polar lipids are phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The DNA G + C content of the type strain is 66.3%. The average nucleotide identity (ANI) and in silico DNA–DNA hybridization (dDDH) relatedness values between strain TW-1T and closest members were below the threshold value for species demarcation. The genome size is 7,051,197 bp along with 46 contigs and 5,977 protein-coding genes. The genome showed 5 putative biosynthetic gene clusters (BGCs) that are responsible for different secondary metabolites. Cluster 2 showed thiopeptide BGC with no known cluster blast, indicating TW-1T might produce novel antimicrobial agent. The antimicrobial assessment also showed that strain TW-1T possessed inhibitory activity against Gram-negative pathogens (Escherichia coli and Pseudomonas aeruginosa). This is the first report of the species in the genus Massilia which produces antimicrobial compounds. Based on the polyphasic study, strain TW-1T represents novel species in the genus Massilia, for which the name Massilia antibiotica sp. nov. is proposed. The type strain is TW-1T (= KACC 21627T = NBRC 114363T).Ram Hari DahalDhiraj Kumar ChaudharyJaisoo KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ram Hari Dahal Dhiraj Kumar Chaudhary Jaisoo Kim Genome insight and description of antibiotic producing Massilia antibiotica sp. nov., isolated from oil-contaminated soil
description	Abstract An ivory-coloured, motile, Gram-stain-negative bacterium, designated TW-1T was isolated from oil-contaminated experimental soil in Kyonggi University. The phylogenetic analysis based on 16S rRNA gene sequence revealed, strain TW-1T formed a lineage within the family Oxalobacteraceae and clustered as members of the genus Massilia. The closest members were M. pinisoli T33T (98.8% sequence similarity), M. putida 6NM-7T (98.6%), M. arvi THG-RS2OT (98.5%), M. phosphatilytica 12-OD1T (98.3%) and M. niastensis 5516S-1T (98.2%). The sole respiratory quinone is ubiquinone-8. The major cellular fatty acids are hexadeconic acid, cis-9, methylenehexadeconic acid, summed feature 3 and summed feature 8. The major polar lipids are phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The DNA G + C content of the type strain is 66.3%. The average nucleotide identity (ANI) and in silico DNA–DNA hybridization (dDDH) relatedness values between strain TW-1T and closest members were below the threshold value for species demarcation. The genome size is 7,051,197 bp along with 46 contigs and 5,977 protein-coding genes. The genome showed 5 putative biosynthetic gene clusters (BGCs) that are responsible for different secondary metabolites. Cluster 2 showed thiopeptide BGC with no known cluster blast, indicating TW-1T might produce novel antimicrobial agent. The antimicrobial assessment also showed that strain TW-1T possessed inhibitory activity against Gram-negative pathogens (Escherichia coli and Pseudomonas aeruginosa). This is the first report of the species in the genus Massilia which produces antimicrobial compounds. Based on the polyphasic study, strain TW-1T represents novel species in the genus Massilia, for which the name Massilia antibiotica sp. nov. is proposed. The type strain is TW-1T (= KACC 21627T = NBRC 114363T).
format	article
author	Ram Hari Dahal Dhiraj Kumar Chaudhary Jaisoo Kim
author_facet	Ram Hari Dahal Dhiraj Kumar Chaudhary Jaisoo Kim
author_sort	Ram Hari Dahal
title	Genome insight and description of antibiotic producing Massilia antibiotica sp. nov., isolated from oil-contaminated soil
title_short	Genome insight and description of antibiotic producing Massilia antibiotica sp. nov., isolated from oil-contaminated soil
title_full	Genome insight and description of antibiotic producing Massilia antibiotica sp. nov., isolated from oil-contaminated soil
title_fullStr	Genome insight and description of antibiotic producing Massilia antibiotica sp. nov., isolated from oil-contaminated soil
title_full_unstemmed	Genome insight and description of antibiotic producing Massilia antibiotica sp. nov., isolated from oil-contaminated soil
title_sort	genome insight and description of antibiotic producing massilia antibiotica sp. nov., isolated from oil-contaminated soil
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ca957062dac345298372e00fd37a0416
work_keys_str_mv	AT ramharidahal genomeinsightanddescriptionofantibioticproducingmassiliaantibioticaspnovisolatedfromoilcontaminatedsoil AT dhirajkumarchaudhary genomeinsightanddescriptionofantibioticproducingmassiliaantibioticaspnovisolatedfromoilcontaminatedsoil AT jaisookim genomeinsightanddescriptionofantibioticproducingmassiliaantibioticaspnovisolatedfromoilcontaminatedsoil
_version_	1718393120289193984

Genome insight and description of antibiotic producing Massilia antibiotica sp. nov., isolated from oil-contaminated soil

Ejemplares similares