Fis is essential for capsule production in Pasteurella multocida and regulates expression of other important virulence factors.

P. multocida is the causative agent of a wide range of diseases of animals, including fowl cholera in poultry and wild birds. Fowl cholera isolates of P. multocida generally express a capsular polysaccharide composed of hyaluronic acid. There have been reports of spontaneous capsule loss in P. multo...

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Autores principales:	Jason A Steen, Jennifer A Steen, Paul Harrison, Torsten Seemann, Ian Wilkie, Marina Harper, Ben Adler, John D Boyce
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ca99b6d0d0314661a48626dd8a7bcce1
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spelling	oai:doaj.org-article:ca99b6d0d0314661a48626dd8a7bcce12021-11-25T05:48:19ZFis is essential for capsule production in Pasteurella multocida and regulates expression of other important virulence factors.1553-73661553-737410.1371/journal.ppat.1000750https://doaj.org/article/ca99b6d0d0314661a48626dd8a7bcce12010-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20140235/pdf/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374P. multocida is the causative agent of a wide range of diseases of animals, including fowl cholera in poultry and wild birds. Fowl cholera isolates of P. multocida generally express a capsular polysaccharide composed of hyaluronic acid. There have been reports of spontaneous capsule loss in P. multocida, but the mechanism by which this occurs has not been determined. In this study, we identified three independent strains that had spontaneously lost the ability to produce capsular polysaccharide. Quantitative RT-PCR showed that these strains had significantly reduced transcription of the capsule biosynthetic genes, but DNA sequence analysis identified no mutations within the capsule biosynthetic locus. However, whole-genome sequencing of paired capsulated and acapsular strains identified a single point mutation within the fis gene in the acapsular strain. Sequencing of fis from two independently derived spontaneous acapsular strains showed that each contained a mutation within fis. Complementation of these strains with an intact copy of fis, predicted to encode a transcriptional regulator, returned capsule expression to all strains. Therefore, expression of a functional Fis protein is essential for capsule expression in P. multocida. DNA microarray analysis of one of the spontaneous fis mutants identified approximately 30 genes as down-regulated in the mutant, including pfhB_2, which encodes a filamentous hemagglutinin, a known P. multocida virulence factor, and plpE, which encodes the cross protective surface antigen PlpE. Therefore these experiments define for the first time a mechanism for spontaneous capsule loss in P. multocida and identify Fis as a critical regulator of capsule expression. Furthermore, Fis is involved in the regulation of a range of other P. multocida genes including important virulence factors.Jason A SteenJennifer A SteenPaul HarrisonTorsten SeemannIan WilkieMarina HarperBen AdlerJohn D BoycePublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 2, p e1000750 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Jason A Steen Jennifer A Steen Paul Harrison Torsten Seemann Ian Wilkie Marina Harper Ben Adler John D Boyce Fis is essential for capsule production in Pasteurella multocida and regulates expression of other important virulence factors.
description	P. multocida is the causative agent of a wide range of diseases of animals, including fowl cholera in poultry and wild birds. Fowl cholera isolates of P. multocida generally express a capsular polysaccharide composed of hyaluronic acid. There have been reports of spontaneous capsule loss in P. multocida, but the mechanism by which this occurs has not been determined. In this study, we identified three independent strains that had spontaneously lost the ability to produce capsular polysaccharide. Quantitative RT-PCR showed that these strains had significantly reduced transcription of the capsule biosynthetic genes, but DNA sequence analysis identified no mutations within the capsule biosynthetic locus. However, whole-genome sequencing of paired capsulated and acapsular strains identified a single point mutation within the fis gene in the acapsular strain. Sequencing of fis from two independently derived spontaneous acapsular strains showed that each contained a mutation within fis. Complementation of these strains with an intact copy of fis, predicted to encode a transcriptional regulator, returned capsule expression to all strains. Therefore, expression of a functional Fis protein is essential for capsule expression in P. multocida. DNA microarray analysis of one of the spontaneous fis mutants identified approximately 30 genes as down-regulated in the mutant, including pfhB_2, which encodes a filamentous hemagglutinin, a known P. multocida virulence factor, and plpE, which encodes the cross protective surface antigen PlpE. Therefore these experiments define for the first time a mechanism for spontaneous capsule loss in P. multocida and identify Fis as a critical regulator of capsule expression. Furthermore, Fis is involved in the regulation of a range of other P. multocida genes including important virulence factors.
format	article
author	Jason A Steen Jennifer A Steen Paul Harrison Torsten Seemann Ian Wilkie Marina Harper Ben Adler John D Boyce
author_facet	Jason A Steen Jennifer A Steen Paul Harrison Torsten Seemann Ian Wilkie Marina Harper Ben Adler John D Boyce
author_sort	Jason A Steen
title	Fis is essential for capsule production in Pasteurella multocida and regulates expression of other important virulence factors.
title_short	Fis is essential for capsule production in Pasteurella multocida and regulates expression of other important virulence factors.
title_full	Fis is essential for capsule production in Pasteurella multocida and regulates expression of other important virulence factors.
title_fullStr	Fis is essential for capsule production in Pasteurella multocida and regulates expression of other important virulence factors.
title_full_unstemmed	Fis is essential for capsule production in Pasteurella multocida and regulates expression of other important virulence factors.
title_sort	fis is essential for capsule production in pasteurella multocida and regulates expression of other important virulence factors.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/ca99b6d0d0314661a48626dd8a7bcce1
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Fis is essential for capsule production in Pasteurella multocida and regulates expression of other important virulence factors.

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