Transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa

Abstract Alfalfa is an important forage crop that is moderately tolerant to salinity; however, little is known about its salt-tolerance mechanisms. We studied root and leaf transcriptomes of a salt-tolerant (G03) and a salt-sensitive (G09) genotype, irrigated with waters of low and high salinities....

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Autores principales:	Rakesh Kaundal, Naveen Duhan, Biswa R. Acharya, Manju V. Pudussery, Jorge F. S. Ferreira, Donald L. Suarez, Devinder Sandhu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ca99ca81225f4f6f844f77653ef19feb
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spelling	oai:doaj.org-article:ca99ca81225f4f6f844f77653ef19feb2021-12-02T13:35:05ZTranscriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa10.1038/s41598-021-84461-w2045-2322https://doaj.org/article/ca99ca81225f4f6f844f77653ef19feb2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84461-whttps://doaj.org/toc/2045-2322Abstract Alfalfa is an important forage crop that is moderately tolerant to salinity; however, little is known about its salt-tolerance mechanisms. We studied root and leaf transcriptomes of a salt-tolerant (G03) and a salt-sensitive (G09) genotype, irrigated with waters of low and high salinities. RNA sequencing led to 1.73 billion high-quality reads that were assembled into 418,480 unigenes; 35% of which were assigned to 57 Gene Ontology annotations. The unigenes were assigned to pathway databases for understanding high-level functions. The comparison of two genotypes suggested that the low salt tolerance index for transpiration rate and stomatal conductance of G03 compared to G09 may be due to its reduced salt uptake under salinity. The differences in shoot biomass between the salt-tolerant and salt-sensitive lines were explained by their differential expressions of genes regulating shoot number. Differentially expressed genes involved in hormone-, calcium-, and redox-signaling, showed treatment- and genotype-specific differences and led to the identification of various candidate genes involved in salinity stress, which can be investigated further to improve salinity tolerance in alfalfa. Validation of RNA-seq results using qRT-PCR displayed a high level of consistency between the two experiments. This study provides valuable insight into the molecular mechanisms regulating salt tolerance in alfalfa.Rakesh KaundalNaveen DuhanBiswa R. AcharyaManju V. PudusseryJorge F. S. FerreiraDonald L. SuarezDevinder SandhuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rakesh Kaundal Naveen Duhan Biswa R. Acharya Manju V. Pudussery Jorge F. S. Ferreira Donald L. Suarez Devinder Sandhu Transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa
description	Abstract Alfalfa is an important forage crop that is moderately tolerant to salinity; however, little is known about its salt-tolerance mechanisms. We studied root and leaf transcriptomes of a salt-tolerant (G03) and a salt-sensitive (G09) genotype, irrigated with waters of low and high salinities. RNA sequencing led to 1.73 billion high-quality reads that were assembled into 418,480 unigenes; 35% of which were assigned to 57 Gene Ontology annotations. The unigenes were assigned to pathway databases for understanding high-level functions. The comparison of two genotypes suggested that the low salt tolerance index for transpiration rate and stomatal conductance of G03 compared to G09 may be due to its reduced salt uptake under salinity. The differences in shoot biomass between the salt-tolerant and salt-sensitive lines were explained by their differential expressions of genes regulating shoot number. Differentially expressed genes involved in hormone-, calcium-, and redox-signaling, showed treatment- and genotype-specific differences and led to the identification of various candidate genes involved in salinity stress, which can be investigated further to improve salinity tolerance in alfalfa. Validation of RNA-seq results using qRT-PCR displayed a high level of consistency between the two experiments. This study provides valuable insight into the molecular mechanisms regulating salt tolerance in alfalfa.
format	article
author	Rakesh Kaundal Naveen Duhan Biswa R. Acharya Manju V. Pudussery Jorge F. S. Ferreira Donald L. Suarez Devinder Sandhu
author_facet	Rakesh Kaundal Naveen Duhan Biswa R. Acharya Manju V. Pudussery Jorge F. S. Ferreira Donald L. Suarez Devinder Sandhu
author_sort	Rakesh Kaundal
title	Transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa
title_short	Transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa
title_full	Transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa
title_fullStr	Transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa
title_full_unstemmed	Transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa
title_sort	transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ca99ca81225f4f6f844f77653ef19feb
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Transcriptional profiling of two contrasting genotypes uncovers molecular mechanisms underlying salt tolerance in alfalfa

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