Pericyte-like progenitors show high immaturity and engraftment potential as compared with mesenchymal stem cells.

Pericyte-like progenitors show high immaturity and engraftment potential as compared with mesenchymal stem cells.

Mesenchymal stem cells (MSCs) and pericyte progenitors (PPs) are both perivascular cells with similar multipotential properties regardless of tissue of origin. We compared the phenotype and function of the 2 cell types derived from the same bone-marrow samples but expanded in their respective media...

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Autores principales: Amina Bouacida, Philippe Rosset, Valérie Trichet, Fabien Guilloton, Nicolas Espagnolle, Thomas Cordonier, Dominique Heymann, Pierre Layrolle, Luc Sensébé, Frédéric Deschaseaux
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Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2012
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Acceso en línea:https://doaj.org/article/caa21bbf6d774926835d3fd776f33dc4
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spelling oai:doaj.org-article:caa21bbf6d774926835d3fd776f33dc42021-11-18T08:09:44ZPericyte-like progenitors show high immaturity and engraftment potential as compared with mesenchymal stem cells.1932-620310.1371/journal.pone.0048648https://doaj.org/article/caa21bbf6d774926835d3fd776f33dc42012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23144918/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Mesenchymal stem cells (MSCs) and pericyte progenitors (PPs) are both perivascular cells with similar multipotential properties regardless of tissue of origin. We compared the phenotype and function of the 2 cell types derived from the same bone-marrow samples but expanded in their respective media - pericyte conditions (endothelial cell growth medium 2 [EGM-2]) for PPs and standard medium (mesenchymal stem cell medium [MSM]) for MSCs. After 3 weeks of culture, whatever the expansion medium, all cells showed similar characteristics (MSC markers and adipo-osteo-chondroblastic differentiation potential), although neuronal potential was greater in EGM-2- than MSM-cultured cells. As compared with MSM-cultured MSCs, EGM-2-cultured PPs showed higher expression of the pericyte-specific antigen 3G5 than α-smooth muscle actin. In addition, EGM-2-cultured PPs showed an immature phenotype, with upregulation of stemness OCT4 and SOX2 proteins and downregulation of markers of osteoblastic, chondroblastic, adipocytic and vascular smooth muscle lineages. Despite having less effective in vitro immunosuppression capacities than standard MSCs, EGM-2-cultured PPs had higher engraftment potentials when combined with biomaterials heterotopically-transplanted in Nude mice. Furthermore, these engrafted cells generated more collagen matrix and were preferentially perivascular or lined trabeculae as compared with MSM-cultured MSCs. In conclusion, EGM-2-cultured PPs are highly immature cells with increased plasticity and engraftment potential.Amina BouacidaPhilippe RossetValérie TrichetFabien GuillotonNicolas EspagnolleThomas CordonierDominique HeymannPierre LayrolleLuc SensébéFrédéric DeschaseauxPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e48648 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Amina Bouacida
Philippe Rosset
Valérie Trichet
Fabien Guilloton
Nicolas Espagnolle
Thomas Cordonier
Dominique Heymann
Pierre Layrolle
Luc Sensébé
Frédéric Deschaseaux
Pericyte-like progenitors show high immaturity and engraftment potential as compared with mesenchymal stem cells.
description Mesenchymal stem cells (MSCs) and pericyte progenitors (PPs) are both perivascular cells with similar multipotential properties regardless of tissue of origin. We compared the phenotype and function of the 2 cell types derived from the same bone-marrow samples but expanded in their respective media - pericyte conditions (endothelial cell growth medium 2 [EGM-2]) for PPs and standard medium (mesenchymal stem cell medium [MSM]) for MSCs. After 3 weeks of culture, whatever the expansion medium, all cells showed similar characteristics (MSC markers and adipo-osteo-chondroblastic differentiation potential), although neuronal potential was greater in EGM-2- than MSM-cultured cells. As compared with MSM-cultured MSCs, EGM-2-cultured PPs showed higher expression of the pericyte-specific antigen 3G5 than α-smooth muscle actin. In addition, EGM-2-cultured PPs showed an immature phenotype, with upregulation of stemness OCT4 and SOX2 proteins and downregulation of markers of osteoblastic, chondroblastic, adipocytic and vascular smooth muscle lineages. Despite having less effective in vitro immunosuppression capacities than standard MSCs, EGM-2-cultured PPs had higher engraftment potentials when combined with biomaterials heterotopically-transplanted in Nude mice. Furthermore, these engrafted cells generated more collagen matrix and were preferentially perivascular or lined trabeculae as compared with MSM-cultured MSCs. In conclusion, EGM-2-cultured PPs are highly immature cells with increased plasticity and engraftment potential.
format article
author Amina Bouacida
Philippe Rosset
Valérie Trichet
Fabien Guilloton
Nicolas Espagnolle
Thomas Cordonier
Dominique Heymann
Pierre Layrolle
Luc Sensébé
Frédéric Deschaseaux
author_facet Amina Bouacida
Philippe Rosset
Valérie Trichet
Fabien Guilloton
Nicolas Espagnolle
Thomas Cordonier
Dominique Heymann
Pierre Layrolle
Luc Sensébé
Frédéric Deschaseaux
author_sort Amina Bouacida
title Pericyte-like progenitors show high immaturity and engraftment potential as compared with mesenchymal stem cells.
title_short Pericyte-like progenitors show high immaturity and engraftment potential as compared with mesenchymal stem cells.
title_full Pericyte-like progenitors show high immaturity and engraftment potential as compared with mesenchymal stem cells.
title_fullStr Pericyte-like progenitors show high immaturity and engraftment potential as compared with mesenchymal stem cells.
title_full_unstemmed Pericyte-like progenitors show high immaturity and engraftment potential as compared with mesenchymal stem cells.
title_sort pericyte-like progenitors show high immaturity and engraftment potential as compared with mesenchymal stem cells.
publisher Public Library of Science (PLoS)
publishDate 2012
url https://doaj.org/article/caa21bbf6d774926835d3fd776f33dc4
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