Gold nanoparticle-based rapid detection and isolation of cells using ligand-receptor chemistry

Abstract Identification and isolation of low-frequency cells of interest from a heterogeneous cell mixture is an important aspect of many diagnostic applications (including enumeration of circulating tumor cells) and is integral to various assays in (cancer) biology. Current techniques typically req...

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Autores principales:	Pradipta Ranjan Rauta, Pavan M. Hallur, Aditya Chaubey
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/caa33480a4e64db78878358943bb982a
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spelling	oai:doaj.org-article:caa33480a4e64db78878358943bb982a2021-12-02T15:08:36ZGold nanoparticle-based rapid detection and isolation of cells using ligand-receptor chemistry10.1038/s41598-018-21068-82045-2322https://doaj.org/article/caa33480a4e64db78878358943bb982a2018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21068-8https://doaj.org/toc/2045-2322Abstract Identification and isolation of low-frequency cells of interest from a heterogeneous cell mixture is an important aspect of many diagnostic applications (including enumeration of circulating tumor cells) and is integral to various assays in (cancer) biology. Current techniques typically require expensive instrumentation and are not amenable to high throughput. Here, we demonstrate a simple and effective platform for cell detection and isolation using gold nanoparticles (Au NPs) conjugated with hyaluronic acid (HA) i.e. Au-PEG-HA NPs. The proposed platform exploits ligand-receptor chemistry to detect/isolate cells with high specificity and efficiency. When the Au-PEG-HA NPs come in contact with cells that express CD44 (the receptor for HA), a clear colorimetric change occurs (along with an accompanying SPR peak shift from 521 nm to 559 nm) in the solution due to NPs-cell interaction. This clearly discernible, colorimetric change can be leveraged by point-of-care devices employed in diagnostic applications. Finally, we show that we can successfully isolate viable cells from a heterogeneous cell population (including from human blood samples) with high specificity, which can be used in further downstream applications. The developed NPs-based platform can be a convenient and cost-efficient alternative for diagnostic applications and for cell isolation or sorting in research laboratories.Pradipta Ranjan RautaPavan M. HallurAditya ChaubeyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pradipta Ranjan Rauta Pavan M. Hallur Aditya Chaubey Gold nanoparticle-based rapid detection and isolation of cells using ligand-receptor chemistry
description	Abstract Identification and isolation of low-frequency cells of interest from a heterogeneous cell mixture is an important aspect of many diagnostic applications (including enumeration of circulating tumor cells) and is integral to various assays in (cancer) biology. Current techniques typically require expensive instrumentation and are not amenable to high throughput. Here, we demonstrate a simple and effective platform for cell detection and isolation using gold nanoparticles (Au NPs) conjugated with hyaluronic acid (HA) i.e. Au-PEG-HA NPs. The proposed platform exploits ligand-receptor chemistry to detect/isolate cells with high specificity and efficiency. When the Au-PEG-HA NPs come in contact with cells that express CD44 (the receptor for HA), a clear colorimetric change occurs (along with an accompanying SPR peak shift from 521 nm to 559 nm) in the solution due to NPs-cell interaction. This clearly discernible, colorimetric change can be leveraged by point-of-care devices employed in diagnostic applications. Finally, we show that we can successfully isolate viable cells from a heterogeneous cell population (including from human blood samples) with high specificity, which can be used in further downstream applications. The developed NPs-based platform can be a convenient and cost-efficient alternative for diagnostic applications and for cell isolation or sorting in research laboratories.
format	article
author	Pradipta Ranjan Rauta Pavan M. Hallur Aditya Chaubey
author_facet	Pradipta Ranjan Rauta Pavan M. Hallur Aditya Chaubey
author_sort	Pradipta Ranjan Rauta
title	Gold nanoparticle-based rapid detection and isolation of cells using ligand-receptor chemistry
title_short	Gold nanoparticle-based rapid detection and isolation of cells using ligand-receptor chemistry
title_full	Gold nanoparticle-based rapid detection and isolation of cells using ligand-receptor chemistry
title_fullStr	Gold nanoparticle-based rapid detection and isolation of cells using ligand-receptor chemistry
title_full_unstemmed	Gold nanoparticle-based rapid detection and isolation of cells using ligand-receptor chemistry
title_sort	gold nanoparticle-based rapid detection and isolation of cells using ligand-receptor chemistry
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/caa33480a4e64db78878358943bb982a
work_keys_str_mv	AT pradiptaranjanrauta goldnanoparticlebasedrapiddetectionandisolationofcellsusingligandreceptorchemistry AT pavanmhallur goldnanoparticlebasedrapiddetectionandisolationofcellsusingligandreceptorchemistry AT adityachaubey goldnanoparticlebasedrapiddetectionandisolationofcellsusingligandreceptorchemistry
_version_	1718388087449452544

Gold nanoparticle-based rapid detection and isolation of cells using ligand-receptor chemistry

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