Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat

Abstract Ocular and periocular traumatisms may result in loss of vision. Hypothermia provides a beneficial intervention for brain and heart conditions and, here, we study whether hypothermia can prevent retinal damage caused by traumatic neuropathy. Intraorbital optic nerve crush (IONC) or sham mani...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Manuel Rey-Funes, Ignacio M. Larrayoz, Daniela S. Contartese, Manuel Soliño, Anibal Sarotto, Martín Bustelo, Martín Bruno, Verónica B. Dorfman, César F. Loidl, Alfredo Martínez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/caa4f68fe4db42288eb93e00e34ea331
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:caa4f68fe4db42288eb93e00e34ea331
record_format	dspace
spelling	oai:doaj.org-article:caa4f68fe4db42288eb93e00e34ea3312021-12-02T15:04:54ZHypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat10.1038/s41598-017-07294-62045-2322https://doaj.org/article/caa4f68fe4db42288eb93e00e34ea3312017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07294-6https://doaj.org/toc/2045-2322Abstract Ocular and periocular traumatisms may result in loss of vision. Hypothermia provides a beneficial intervention for brain and heart conditions and, here, we study whether hypothermia can prevent retinal damage caused by traumatic neuropathy. Intraorbital optic nerve crush (IONC) or sham manipulation was applied to male rats. Some animals were subjected to hypothermia (8 °C) for 3 h following surgery. Thirty days later, animals were subjected to electroretinography and behavioral tests. IONC treatment resulted in amplitude reduction of the b-wave and oscillatory potentials of the electroretinogram, whereas the hypothermic treatment significantly (p < 0.05) reversed this process. Using a descending method of limits in a two-choice visual task apparatus, we demonstrated that hypothermia significantly (p < 0.001) preserved visual acuity. Furthermore, IONC-treated rats had a lower (p < 0.0001) number of retinal ganglion cells and a higher (p < 0.0001) number of TUNEL-positive cells than sham-operated controls. These numbers were significantly (p < 0.0001) corrected by hypothermic treatment. There was a significant (p < 0.001) increase of RNA-binding motif protein 3 (RBM3) and of BCL2 (p < 0.01) mRNA expression in the eyes exposed to hypothermia. In conclusion, hypothermia constitutes an efficacious treatment for traumatic vision-impairing conditions, and the cold-shock protein pathway may be involved in mediating the beneficial effects shown in the retina.Manuel Rey-FunesIgnacio M. LarrayozDaniela S. ContarteseManuel SoliñoAnibal SarottoMartín BusteloMartín BrunoVerónica B. DorfmanCésar F. LoidlAlfredo MartínezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manuel Rey-Funes Ignacio M. Larrayoz Daniela S. Contartese Manuel Soliño Anibal Sarotto Martín Bustelo Martín Bruno Verónica B. Dorfman César F. Loidl Alfredo Martínez Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat
description	Abstract Ocular and periocular traumatisms may result in loss of vision. Hypothermia provides a beneficial intervention for brain and heart conditions and, here, we study whether hypothermia can prevent retinal damage caused by traumatic neuropathy. Intraorbital optic nerve crush (IONC) or sham manipulation was applied to male rats. Some animals were subjected to hypothermia (8 °C) for 3 h following surgery. Thirty days later, animals were subjected to electroretinography and behavioral tests. IONC treatment resulted in amplitude reduction of the b-wave and oscillatory potentials of the electroretinogram, whereas the hypothermic treatment significantly (p < 0.05) reversed this process. Using a descending method of limits in a two-choice visual task apparatus, we demonstrated that hypothermia significantly (p < 0.001) preserved visual acuity. Furthermore, IONC-treated rats had a lower (p < 0.0001) number of retinal ganglion cells and a higher (p < 0.0001) number of TUNEL-positive cells than sham-operated controls. These numbers were significantly (p < 0.0001) corrected by hypothermic treatment. There was a significant (p < 0.001) increase of RNA-binding motif protein 3 (RBM3) and of BCL2 (p < 0.01) mRNA expression in the eyes exposed to hypothermia. In conclusion, hypothermia constitutes an efficacious treatment for traumatic vision-impairing conditions, and the cold-shock protein pathway may be involved in mediating the beneficial effects shown in the retina.
format	article
author	Manuel Rey-Funes Ignacio M. Larrayoz Daniela S. Contartese Manuel Soliño Anibal Sarotto Martín Bustelo Martín Bruno Verónica B. Dorfman César F. Loidl Alfredo Martínez
author_facet	Manuel Rey-Funes Ignacio M. Larrayoz Daniela S. Contartese Manuel Soliño Anibal Sarotto Martín Bustelo Martín Bruno Verónica B. Dorfman César F. Loidl Alfredo Martínez
author_sort	Manuel Rey-Funes
title	Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat
title_short	Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat
title_full	Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat
title_fullStr	Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat
title_full_unstemmed	Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat
title_sort	hypothermia prevents retinal damage generated by optic nerve trauma in the rat
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/caa4f68fe4db42288eb93e00e34ea331
work_keys_str_mv	AT manuelreyfunes hypothermiapreventsretinaldamagegeneratedbyopticnervetraumaintherat AT ignaciomlarrayoz hypothermiapreventsretinaldamagegeneratedbyopticnervetraumaintherat AT danielascontartese hypothermiapreventsretinaldamagegeneratedbyopticnervetraumaintherat AT manuelsolino hypothermiapreventsretinaldamagegeneratedbyopticnervetraumaintherat AT anibalsarotto hypothermiapreventsretinaldamagegeneratedbyopticnervetraumaintherat AT martinbustelo hypothermiapreventsretinaldamagegeneratedbyopticnervetraumaintherat AT martinbruno hypothermiapreventsretinaldamagegeneratedbyopticnervetraumaintherat AT veronicabdorfman hypothermiapreventsretinaldamagegeneratedbyopticnervetraumaintherat AT cesarfloidl hypothermiapreventsretinaldamagegeneratedbyopticnervetraumaintherat AT alfredomartinez hypothermiapreventsretinaldamagegeneratedbyopticnervetraumaintherat
_version_	1718389042826969088

Hypothermia Prevents Retinal Damage Generated by Optic Nerve Trauma in the Rat

Ejemplares similares