Inhibition of the PERK/TXNIP/NLRP3 Axis by Baicalin Reduces NLRP3 Inflammasome-Mediated Pyroptosis in Macrophages Infected with Mycobacterium tuberculosis
Mycobacterium tuberculosis (Mtb) remains a significant threat to global health as it induces granuloma and systemic inflammatory responses during active tuberculosis. Mtb can induce macrophage pyroptosis, leading to the release of IL-1β and tissue damage, promoting its spread. Here, we established a...
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Hindawi Limited
2021
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oai:doaj.org-article:caa51d5f5aae48569a78b0279f8203242021-11-22T01:11:19ZInhibition of the PERK/TXNIP/NLRP3 Axis by Baicalin Reduces NLRP3 Inflammasome-Mediated Pyroptosis in Macrophages Infected with Mycobacterium tuberculosis1466-186110.1155/2021/1805147https://doaj.org/article/caa51d5f5aae48569a78b0279f8203242021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/1805147https://doaj.org/toc/1466-1861Mycobacterium tuberculosis (Mtb) remains a significant threat to global health as it induces granuloma and systemic inflammatory responses during active tuberculosis. Mtb can induce macrophage pyroptosis, leading to the release of IL-1β and tissue damage, promoting its spread. Here, we established an in vitro Mtb-infected macrophage model to seek an effective antipyroptosis agent. Baicalin, isolated from Radix Scutellariae, was found to reduce pyroptosis in Mtb-infected macrophages. Baicalin could inhibit activation of the PERK/eIF2α pathway and thus downregulates TXNIP expression and subsequently reduces activation of the NLRP3 inflammasome, resulting in reduced pyroptosis in Mtb-infected macrophages. In conclusion, baicalin reduced pyroptosis by inhibiting the PERK/TXNIP/NLRP3 axis and might thus be a new adjuvant host-directed therapy (HDT) drug.Yan FuJingjing ShenYinhong LiFanglin LiuBangzuo NingYuejuan ZhengXin JiangHindawi LimitedarticlePathologyRB1-214ENMediators of Inflammation, Vol 2021 (2021) |
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DOAJ |
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Pathology RB1-214 |
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Pathology RB1-214 Yan Fu Jingjing Shen Yinhong Li Fanglin Liu Bangzuo Ning Yuejuan Zheng Xin Jiang Inhibition of the PERK/TXNIP/NLRP3 Axis by Baicalin Reduces NLRP3 Inflammasome-Mediated Pyroptosis in Macrophages Infected with Mycobacterium tuberculosis |
| description |
Mycobacterium tuberculosis (Mtb) remains a significant threat to global health as it induces granuloma and systemic inflammatory responses during active tuberculosis. Mtb can induce macrophage pyroptosis, leading to the release of IL-1β and tissue damage, promoting its spread. Here, we established an in vitro Mtb-infected macrophage model to seek an effective antipyroptosis agent. Baicalin, isolated from Radix Scutellariae, was found to reduce pyroptosis in Mtb-infected macrophages. Baicalin could inhibit activation of the PERK/eIF2α pathway and thus downregulates TXNIP expression and subsequently reduces activation of the NLRP3 inflammasome, resulting in reduced pyroptosis in Mtb-infected macrophages. In conclusion, baicalin reduced pyroptosis by inhibiting the PERK/TXNIP/NLRP3 axis and might thus be a new adjuvant host-directed therapy (HDT) drug. |
| format |
article |
| author |
Yan Fu Jingjing Shen Yinhong Li Fanglin Liu Bangzuo Ning Yuejuan Zheng Xin Jiang |
| author_facet |
Yan Fu Jingjing Shen Yinhong Li Fanglin Liu Bangzuo Ning Yuejuan Zheng Xin Jiang |
| author_sort |
Yan Fu |
| title |
Inhibition of the PERK/TXNIP/NLRP3 Axis by Baicalin Reduces NLRP3 Inflammasome-Mediated Pyroptosis in Macrophages Infected with Mycobacterium tuberculosis |
| title_short |
Inhibition of the PERK/TXNIP/NLRP3 Axis by Baicalin Reduces NLRP3 Inflammasome-Mediated Pyroptosis in Macrophages Infected with Mycobacterium tuberculosis |
| title_full |
Inhibition of the PERK/TXNIP/NLRP3 Axis by Baicalin Reduces NLRP3 Inflammasome-Mediated Pyroptosis in Macrophages Infected with Mycobacterium tuberculosis |
| title_fullStr |
Inhibition of the PERK/TXNIP/NLRP3 Axis by Baicalin Reduces NLRP3 Inflammasome-Mediated Pyroptosis in Macrophages Infected with Mycobacterium tuberculosis |
| title_full_unstemmed |
Inhibition of the PERK/TXNIP/NLRP3 Axis by Baicalin Reduces NLRP3 Inflammasome-Mediated Pyroptosis in Macrophages Infected with Mycobacterium tuberculosis |
| title_sort |
inhibition of the perk/txnip/nlrp3 axis by baicalin reduces nlrp3 inflammasome-mediated pyroptosis in macrophages infected with mycobacterium tuberculosis |
| publisher |
Hindawi Limited |
| publishDate |
2021 |
| url |
https://doaj.org/article/caa51d5f5aae48569a78b0279f820324 |
| work_keys_str_mv |
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