Effects of short- and long-term regular exercise on reproductive tissue in streptozotocin-induced diabetic male Wistar rats

Objective. Reproductive disorders are one of the complications of diabetes mellitus. Since conflicting results have been obtained from different studies, which examined serum levels of cytokines in patients with diabetes, and considering the fact that the origin of cytokines cannot be accurately det...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mogaddami Zahra, Sheikhzadeh Farzam, Hatami Homeira, Khojasteh Seyed Mahdi Banan, Khajehnasiri Nazli, Hemmati Ali Reza Ali, Dastranj Ali
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2018
Materias:	regular exercise type 1 diabetes histometry tnf-α il-1 spermatogonial and sertoli cells epithelial thickness seminiferous tubule Diseases of the endocrine glands. Clinical endocrinology RC648-665
Acceso en línea:	https://doaj.org/article/caa7b581067f4665b5b5b52b65f85cf0
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:caa7b581067f4665b5b5b52b65f85cf0
record_format	dspace
spelling	oai:doaj.org-article:caa7b581067f4665b5b5b52b65f85cf02021-12-02T17:49:32ZEffects of short- and long-term regular exercise on reproductive tissue in streptozotocin-induced diabetic male Wistar rats1336-032910.2478/enr-2018-0021https://doaj.org/article/caa7b581067f4665b5b5b52b65f85cf02018-10-01T00:00:00Zhttps://doi.org/10.2478/enr-2018-0021https://doaj.org/toc/1336-0329Objective. Reproductive disorders are one of the complications of diabetes mellitus. Since conflicting results have been obtained from different studies, which examined serum levels of cytokines in patients with diabetes, and considering the fact that the origin of cytokines cannot be accurately determined from their serum changes, attempts were made in the present study to study histological changes and testicular tissue levels of TNF-α and IL-1 in rats treated with exercise. Considering the effects of exercise in reducing blood sugar level and its complications, two types of short-term and long-term regular exercises were also considered to evaluate their effects on male reproductive tissues. Methods. In this study, 60 male rats with the weight range of 250±50 g were used and were randomly divided into six groups (10 rats each). Healthy groups included sedentary control group, and groups treated with two and eight weeks of exercise. Rats with type 1 diabetes (induced by streptozotocin) included sedentary control group, groups treated with two and eight weeks of exercise (six groups). All groups were evaluated in terms of testicular tissue levels of TNF-α and IL-1 using ELISA and the histometry of spermatogonia, primary spermatocytes, Sertoli cells, epithelial thickness, diameter of veins, and thickness of the seminiferous tubule. Results. Histological changes resulting from diabetes, particularly in the diameter of testicular veins and a number of cells, including Sertoli, highlights the important fact that tissue perfusion in patients with diabetes is especially crucial, in a way that exercise proved useful for tissue structures by offsetting this complication. Measurement of the cytokines IL-1 and TNF-α in the current study showed that perfusion problems are more important in diabetic complications than inflammatory factors. Conclusions. The main result of this research is recommendation of investigating the tissue of interest for diagnosis of diabetes complications, measuring inflammatory mediators of tissue rather than evaluating their serum concentrations, and focusing on vascular complications as a major complication of diabetes. Furthermore, regular exercise could help improve the function of reproductive organs in healthy groups and prevent diabetes infertility complications to an acceptable degree in diabetic groups.Mogaddami ZahraSheikhzadeh FarzamHatami HomeiraKhojasteh Seyed Mahdi BananKhajehnasiri NazliHemmati Ali Reza AliDastranj AliSciendoarticleregular exercisetype 1 diabeteshistometrytnf-αil-1spermatogonial and sertoli cellsepithelial thicknessseminiferous tubuleDiseases of the endocrine glands. Clinical endocrinologyRC648-665ENEndocrine Regulations, Vol 52, Iss 4, Pp 167-175 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	regular exercise type 1 diabetes histometry tnf-α il-1 spermatogonial and sertoli cells epithelial thickness seminiferous tubule Diseases of the endocrine glands. Clinical endocrinology RC648-665
spellingShingle	regular exercise type 1 diabetes histometry tnf-α il-1 spermatogonial and sertoli cells epithelial thickness seminiferous tubule Diseases of the endocrine glands. Clinical endocrinology RC648-665 Mogaddami Zahra Sheikhzadeh Farzam Hatami Homeira Khojasteh Seyed Mahdi Banan Khajehnasiri Nazli Hemmati Ali Reza Ali Dastranj Ali Effects of short- and long-term regular exercise on reproductive tissue in streptozotocin-induced diabetic male Wistar rats
description	Objective. Reproductive disorders are one of the complications of diabetes mellitus. Since conflicting results have been obtained from different studies, which examined serum levels of cytokines in patients with diabetes, and considering the fact that the origin of cytokines cannot be accurately determined from their serum changes, attempts were made in the present study to study histological changes and testicular tissue levels of TNF-α and IL-1 in rats treated with exercise. Considering the effects of exercise in reducing blood sugar level and its complications, two types of short-term and long-term regular exercises were also considered to evaluate their effects on male reproductive tissues. Methods. In this study, 60 male rats with the weight range of 250±50 g were used and were randomly divided into six groups (10 rats each). Healthy groups included sedentary control group, and groups treated with two and eight weeks of exercise. Rats with type 1 diabetes (induced by streptozotocin) included sedentary control group, groups treated with two and eight weeks of exercise (six groups). All groups were evaluated in terms of testicular tissue levels of TNF-α and IL-1 using ELISA and the histometry of spermatogonia, primary spermatocytes, Sertoli cells, epithelial thickness, diameter of veins, and thickness of the seminiferous tubule. Results. Histological changes resulting from diabetes, particularly in the diameter of testicular veins and a number of cells, including Sertoli, highlights the important fact that tissue perfusion in patients with diabetes is especially crucial, in a way that exercise proved useful for tissue structures by offsetting this complication. Measurement of the cytokines IL-1 and TNF-α in the current study showed that perfusion problems are more important in diabetic complications than inflammatory factors. Conclusions. The main result of this research is recommendation of investigating the tissue of interest for diagnosis of diabetes complications, measuring inflammatory mediators of tissue rather than evaluating their serum concentrations, and focusing on vascular complications as a major complication of diabetes. Furthermore, regular exercise could help improve the function of reproductive organs in healthy groups and prevent diabetes infertility complications to an acceptable degree in diabetic groups.
format	article
author	Mogaddami Zahra Sheikhzadeh Farzam Hatami Homeira Khojasteh Seyed Mahdi Banan Khajehnasiri Nazli Hemmati Ali Reza Ali Dastranj Ali
author_facet	Mogaddami Zahra Sheikhzadeh Farzam Hatami Homeira Khojasteh Seyed Mahdi Banan Khajehnasiri Nazli Hemmati Ali Reza Ali Dastranj Ali
author_sort	Mogaddami Zahra
title	Effects of short- and long-term regular exercise on reproductive tissue in streptozotocin-induced diabetic male Wistar rats
title_short	Effects of short- and long-term regular exercise on reproductive tissue in streptozotocin-induced diabetic male Wistar rats
title_full	Effects of short- and long-term regular exercise on reproductive tissue in streptozotocin-induced diabetic male Wistar rats
title_fullStr	Effects of short- and long-term regular exercise on reproductive tissue in streptozotocin-induced diabetic male Wistar rats
title_full_unstemmed	Effects of short- and long-term regular exercise on reproductive tissue in streptozotocin-induced diabetic male Wistar rats
title_sort	effects of short- and long-term regular exercise on reproductive tissue in streptozotocin-induced diabetic male wistar rats
publisher	Sciendo
publishDate	2018
url	https://doaj.org/article/caa7b581067f4665b5b5b52b65f85cf0
work_keys_str_mv	AT mogaddamizahra effectsofshortandlongtermregularexerciseonreproductivetissueinstreptozotocininduceddiabeticmalewistarrats AT sheikhzadehfarzam effectsofshortandlongtermregularexerciseonreproductivetissueinstreptozotocininduceddiabeticmalewistarrats AT hatamihomeira effectsofshortandlongtermregularexerciseonreproductivetissueinstreptozotocininduceddiabeticmalewistarrats AT khojastehseyedmahdibanan effectsofshortandlongtermregularexerciseonreproductivetissueinstreptozotocininduceddiabeticmalewistarrats AT khajehnasirinazli effectsofshortandlongtermregularexerciseonreproductivetissueinstreptozotocininduceddiabeticmalewistarrats AT hemmatialirezaali effectsofshortandlongtermregularexerciseonreproductivetissueinstreptozotocininduceddiabeticmalewistarrats AT dastranjali effectsofshortandlongtermregularexerciseonreproductivetissueinstreptozotocininduceddiabeticmalewistarrats
_version_	1718379434457694208

Effects of short- and long-term regular exercise on reproductive tissue in streptozotocin-induced diabetic male Wistar rats

Ejemplares similares