Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia

Abstract Pilus has been recently associated with pneumococcal pathogenesis in humans. The information regarding piliated isolates in Malaysia is scarce, especially in the less developed states on the east coast of Peninsular Malaysia. Therefore, we studied the characteristics of pneumococci, includi...

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Autores principales:	Nurul Diana Dzaraly, Mohd Nasir Mohd Desa, AbdulRahman Muthanna, Siti Norbaya Masri, Niazlin Mohd Taib, Zarizal Suhaili, Nurshahira Sulaiman, Nurul Hana Zainal Baharin, Cheah Yun Shuan, Zarina Ariffin, Nor Iza A. Rahman, Farahiyah Mohd Rani, Navindra Kumari Palanisamy, Tuan Suhaila Tuan Soh, Fatimah Haslina Abdullah
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/caab006c301a4c3885788a5f67decd74
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spelling	oai:doaj.org-article:caab006c301a4c3885788a5f67decd742021-12-02T18:03:21ZAntimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia10.1038/s41598-021-87428-z2045-2322https://doaj.org/article/caab006c301a4c3885788a5f67decd742021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87428-zhttps://doaj.org/toc/2045-2322Abstract Pilus has been recently associated with pneumococcal pathogenesis in humans. The information regarding piliated isolates in Malaysia is scarce, especially in the less developed states on the east coast of Peninsular Malaysia. Therefore, we studied the characteristics of pneumococci, including the piliated isolates, in relation to antimicrobial susceptibility, serotypes, and genotypes at a major tertiary hospital on the east coast of Peninsular Malaysia. A total of 100 clinical isolates collected between September 2017 and December 2019 were subjected to serotyping, antimicrobial susceptibility test, and detection of pneumococcal virulence and pilus genes. Multilocus sequence typing (MLST) and phylogenetic analysis were performed only for piliated strains. The most frequent serotypes were 14 (17%), 6A/B (16%), 23F (12%), 19A (11%), and 19F (11%). The majority of isolates were resistant to erythromycin (42%), tetracycline (37%), and trimethoprim-sulfamethoxazole (24%). Piliated isolates occurred in a proportion of 19%; 47.3% of them were multidrug-resistant (MDR) and a majority had serotype 19F. This study showed ST236 was the most predominant sequence type (ST) among piliated isolates, which was related to PMEN clone Taiwan19F-14 (CC271). In the phylogenetic analysis, the piliated isolates were grouped into three major clades supported with 100% bootstrap values. Most piliated isolates belonged to internationally disseminated clones of S. pneumoniae, but pneumococcal conjugate vaccines (PCVs) have the potential to control them.Nurul Diana DzaralyMohd Nasir Mohd DesaAbdulRahman MuthannaSiti Norbaya MasriNiazlin Mohd TaibZarizal SuhailiNurshahira SulaimanNurul Hana Zainal BaharinCheah Yun ShuanZarina AriffinNor Iza A. RahmanFarahiyah Mohd RaniNavindra Kumari PalanisamyTuan Suhaila Tuan SohFatimah Haslina AbdullahNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nurul Diana Dzaraly Mohd Nasir Mohd Desa AbdulRahman Muthanna Siti Norbaya Masri Niazlin Mohd Taib Zarizal Suhaili Nurshahira Sulaiman Nurul Hana Zainal Baharin Cheah Yun Shuan Zarina Ariffin Nor Iza A. Rahman Farahiyah Mohd Rani Navindra Kumari Palanisamy Tuan Suhaila Tuan Soh Fatimah Haslina Abdullah Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia
description	Abstract Pilus has been recently associated with pneumococcal pathogenesis in humans. The information regarding piliated isolates in Malaysia is scarce, especially in the less developed states on the east coast of Peninsular Malaysia. Therefore, we studied the characteristics of pneumococci, including the piliated isolates, in relation to antimicrobial susceptibility, serotypes, and genotypes at a major tertiary hospital on the east coast of Peninsular Malaysia. A total of 100 clinical isolates collected between September 2017 and December 2019 were subjected to serotyping, antimicrobial susceptibility test, and detection of pneumococcal virulence and pilus genes. Multilocus sequence typing (MLST) and phylogenetic analysis were performed only for piliated strains. The most frequent serotypes were 14 (17%), 6A/B (16%), 23F (12%), 19A (11%), and 19F (11%). The majority of isolates were resistant to erythromycin (42%), tetracycline (37%), and trimethoprim-sulfamethoxazole (24%). Piliated isolates occurred in a proportion of 19%; 47.3% of them were multidrug-resistant (MDR) and a majority had serotype 19F. This study showed ST236 was the most predominant sequence type (ST) among piliated isolates, which was related to PMEN clone Taiwan19F-14 (CC271). In the phylogenetic analysis, the piliated isolates were grouped into three major clades supported with 100% bootstrap values. Most piliated isolates belonged to internationally disseminated clones of S. pneumoniae, but pneumococcal conjugate vaccines (PCVs) have the potential to control them.
format	article
author	Nurul Diana Dzaraly Mohd Nasir Mohd Desa AbdulRahman Muthanna Siti Norbaya Masri Niazlin Mohd Taib Zarizal Suhaili Nurshahira Sulaiman Nurul Hana Zainal Baharin Cheah Yun Shuan Zarina Ariffin Nor Iza A. Rahman Farahiyah Mohd Rani Navindra Kumari Palanisamy Tuan Suhaila Tuan Soh Fatimah Haslina Abdullah
author_facet	Nurul Diana Dzaraly Mohd Nasir Mohd Desa AbdulRahman Muthanna Siti Norbaya Masri Niazlin Mohd Taib Zarizal Suhaili Nurshahira Sulaiman Nurul Hana Zainal Baharin Cheah Yun Shuan Zarina Ariffin Nor Iza A. Rahman Farahiyah Mohd Rani Navindra Kumari Palanisamy Tuan Suhaila Tuan Soh Fatimah Haslina Abdullah
author_sort	Nurul Diana Dzaraly
title	Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia
title_short	Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia
title_full	Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia
title_fullStr	Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia
title_full_unstemmed	Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia
title_sort	antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, peninsular malaysia
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/caab006c301a4c3885788a5f67decd74
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Antimicrobial susceptibility, serotype distribution, virulence profile and molecular typing of piliated clinical isolates of pneumococci from east coast, Peninsular Malaysia

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