Novel Cyclovirus Species in Dogs with Hemorrhagic Gastroenteritis

Nested PCRs with circovirus/cyclovirus pan-<i>rep</i> (replicase gene) primers detected eukaryotic circular Rep-encoding single-stranded DNA (CRESS DNA) viruses in three (samples CN9E, CN16E and CN34) of 18 canine parvovirus-2-positive fecal samples from household dogs with hemorrhagic g...

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Autores principales:	Kerry Gainor, Yashpal S. Malik, Souvik Ghosh
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cyclovirus domestic dogs complete genome analysis novel species Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/caac31104d6246b29db4cbed006aa8a4
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spelling	oai:doaj.org-article:caac31104d6246b29db4cbed006aa8a42021-11-25T19:12:52ZNovel Cyclovirus Species in Dogs with Hemorrhagic Gastroenteritis10.3390/v131121551999-4915https://doaj.org/article/caac31104d6246b29db4cbed006aa8a42021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2155https://doaj.org/toc/1999-4915Nested PCRs with circovirus/cyclovirus pan-<i>rep</i> (replicase gene) primers detected eukaryotic circular Rep-encoding single-stranded DNA (CRESS DNA) viruses in three (samples CN9E, CN16E and CN34) of 18 canine parvovirus-2-positive fecal samples from household dogs with hemorrhagic gastroenteritis on the Caribbean island of Nevis. The complete genomes of CRESS DNA virus CN9E, CN16E and CN34 were determined by inverse nested PCRs. Based on (i) genome organization, (ii) location of the putative origin of replication, (iii) pairwise genome-wide sequence identities, (iv) the presence of conserved motifs in the putative replication-associated protein (Rep) and the arginine-rich region in the amino terminus of the putative capsid protein (Cp) and (v) a phylogenetic analysis, CN9E, CN16E and CN34 were classified as cycloviruses. Canine-associated cycloviruses CN16E and CN34 were closely related to each other and shared low genome-wide nucleotide (59.642–59.704%), deduced Rep (35.018–35.379%) and Cp (26.601%) amino acid sequence identities with CN9E. All the three canine-associated cycloviruses shared < 80% genome-wide pairwise nucleotide sequence identities with cycloviruses from other animals/environmental samples, constituting two novel species (CN9E and CN16E/34) within the genus <i>Cyclovirus</i>. Considering the feeding habits of dogs, we could not determine whether the cycloviruses were of dietary origin or infected the host. Interestingly, the CN9E putative Rep-encoding open reading frame was found to use the invertebrate mitochondrial genetic code with an alternative initiation codon (ATA) for translation, corroborating the hypothesis that cycloviruses are actually arthropod-infecting viruses. To our knowledge, this is the first report on the detection and complete genome analysis of cycloviruses from domestic dogs.Kerry GainorYashpal S. MalikSouvik GhoshMDPI AGarticlecyclovirusdomestic dogscomplete genome analysisnovel speciesMicrobiologyQR1-502ENViruses, Vol 13, Iss 2155, p 2155 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cyclovirus domestic dogs complete genome analysis novel species Microbiology QR1-502
spellingShingle	cyclovirus domestic dogs complete genome analysis novel species Microbiology QR1-502 Kerry Gainor Yashpal S. Malik Souvik Ghosh Novel Cyclovirus Species in Dogs with Hemorrhagic Gastroenteritis
description	Nested PCRs with circovirus/cyclovirus pan-<i>rep</i> (replicase gene) primers detected eukaryotic circular Rep-encoding single-stranded DNA (CRESS DNA) viruses in three (samples CN9E, CN16E and CN34) of 18 canine parvovirus-2-positive fecal samples from household dogs with hemorrhagic gastroenteritis on the Caribbean island of Nevis. The complete genomes of CRESS DNA virus CN9E, CN16E and CN34 were determined by inverse nested PCRs. Based on (i) genome organization, (ii) location of the putative origin of replication, (iii) pairwise genome-wide sequence identities, (iv) the presence of conserved motifs in the putative replication-associated protein (Rep) and the arginine-rich region in the amino terminus of the putative capsid protein (Cp) and (v) a phylogenetic analysis, CN9E, CN16E and CN34 were classified as cycloviruses. Canine-associated cycloviruses CN16E and CN34 were closely related to each other and shared low genome-wide nucleotide (59.642–59.704%), deduced Rep (35.018–35.379%) and Cp (26.601%) amino acid sequence identities with CN9E. All the three canine-associated cycloviruses shared < 80% genome-wide pairwise nucleotide sequence identities with cycloviruses from other animals/environmental samples, constituting two novel species (CN9E and CN16E/34) within the genus <i>Cyclovirus</i>. Considering the feeding habits of dogs, we could not determine whether the cycloviruses were of dietary origin or infected the host. Interestingly, the CN9E putative Rep-encoding open reading frame was found to use the invertebrate mitochondrial genetic code with an alternative initiation codon (ATA) for translation, corroborating the hypothesis that cycloviruses are actually arthropod-infecting viruses. To our knowledge, this is the first report on the detection and complete genome analysis of cycloviruses from domestic dogs.
format	article
author	Kerry Gainor Yashpal S. Malik Souvik Ghosh
author_facet	Kerry Gainor Yashpal S. Malik Souvik Ghosh
author_sort	Kerry Gainor
title	Novel Cyclovirus Species in Dogs with Hemorrhagic Gastroenteritis
title_short	Novel Cyclovirus Species in Dogs with Hemorrhagic Gastroenteritis
title_full	Novel Cyclovirus Species in Dogs with Hemorrhagic Gastroenteritis
title_fullStr	Novel Cyclovirus Species in Dogs with Hemorrhagic Gastroenteritis
title_full_unstemmed	Novel Cyclovirus Species in Dogs with Hemorrhagic Gastroenteritis
title_sort	novel cyclovirus species in dogs with hemorrhagic gastroenteritis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/caac31104d6246b29db4cbed006aa8a4
work_keys_str_mv	AT kerrygainor novelcyclovirusspeciesindogswithhemorrhagicgastroenteritis AT yashpalsmalik novelcyclovirusspeciesindogswithhemorrhagicgastroenteritis AT souvikghosh novelcyclovirusspeciesindogswithhemorrhagicgastroenteritis
_version_	1718410183294582784

Novel Cyclovirus Species in Dogs with Hemorrhagic Gastroenteritis

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