CLINICAL AND DIAGNOSTIC SIGNIFICANCE OF DEOXYRIBONUCLEASE ACTIVITY OF POLYCLONAL IgG AND BLOOD SERA IN PATIENTS WITH SPONDYLOARTHRITIS

Abstract. A group of 266 patients with spondyloarthritidis and 69 healthy persons were included in our study. IgG preparations were isolated from blood sera by a combined rivanol/affine chromatography technique. Homogeneity of IgGs was tested by means of SDS-PAGE. Serum samples from patients and hea...

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Autores principales:	E. V. Kunder, M. V. Volkova, I. I. Generalov, G. A. Nevinsky
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	spondyloarthritidis polyclonal igg blood serum deoxyribonuclease activity Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/caad527e08484104bc691fd6822689ac
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spelling	oai:doaj.org-article:caad527e08484104bc691fd6822689ac2021-11-18T08:03:41ZCLINICAL AND DIAGNOSTIC SIGNIFICANCE OF DEOXYRIBONUCLEASE ACTIVITY OF POLYCLONAL IgG AND BLOOD SERA IN PATIENTS WITH SPONDYLOARTHRITIS1563-06252313-741X10.15789/1563-0625-2012-4-5-337-346https://doaj.org/article/caad527e08484104bc691fd6822689ac2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/459https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. A group of 266 patients with spondyloarthritidis and 69 healthy persons were included in our study. IgG preparations were isolated from blood sera by a combined rivanol/affine chromatography technique. Homogeneity of IgGs was tested by means of SDS-PAGE. Serum samples from patients and healthy persons, and IgG subclasses 1, 2 and 4 were tested for DNAse activity. A method of DNAse activity measurement was based on rivanol capacity to form a clot with DNA. We have found highly significant differences between the levels of DNAse activity associated with IgG preparations and in blood sera from patients with spondyloarthritidis, and healthy donors (p < 0,0001). DNAse activity of IgG and sera in patients with psoriatic arthritis was higher than in patients with reactive arthritis and ankylosing spondylitis (p < 0,0001). Multiple correlations were revealed between DNAse activity of IgG, blood serum, clinical signs of psoriatic arthritis, reactive arthritis, ankylosing spondylitis, and laboratory findings. We have developed novel tests for differential diagnosis between various disorders, e.g., spondyloarthritidis, based on IgG and serum-associated DNAse activity, corresponding to the criteris of useful and very useful diagnostic tests in rheumatology.E. V. KunderM. V. VolkovaI. I. GeneralovG. A. NevinskySPb RAACIarticlespondyloarthritidispolyclonal iggblood serumdeoxyribonuclease activityImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 14, Iss 4-5, Pp 337-346 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	spondyloarthritidis polyclonal igg blood serum deoxyribonuclease activity Immunologic diseases. Allergy RC581-607
spellingShingle	spondyloarthritidis polyclonal igg blood serum deoxyribonuclease activity Immunologic diseases. Allergy RC581-607 E. V. Kunder M. V. Volkova I. I. Generalov G. A. Nevinsky CLINICAL AND DIAGNOSTIC SIGNIFICANCE OF DEOXYRIBONUCLEASE ACTIVITY OF POLYCLONAL IgG AND BLOOD SERA IN PATIENTS WITH SPONDYLOARTHRITIS
description	Abstract. A group of 266 patients with spondyloarthritidis and 69 healthy persons were included in our study. IgG preparations were isolated from blood sera by a combined rivanol/affine chromatography technique. Homogeneity of IgGs was tested by means of SDS-PAGE. Serum samples from patients and healthy persons, and IgG subclasses 1, 2 and 4 were tested for DNAse activity. A method of DNAse activity measurement was based on rivanol capacity to form a clot with DNA. We have found highly significant differences between the levels of DNAse activity associated with IgG preparations and in blood sera from patients with spondyloarthritidis, and healthy donors (p < 0,0001). DNAse activity of IgG and sera in patients with psoriatic arthritis was higher than in patients with reactive arthritis and ankylosing spondylitis (p < 0,0001). Multiple correlations were revealed between DNAse activity of IgG, blood serum, clinical signs of psoriatic arthritis, reactive arthritis, ankylosing spondylitis, and laboratory findings. We have developed novel tests for differential diagnosis between various disorders, e.g., spondyloarthritidis, based on IgG and serum-associated DNAse activity, corresponding to the criteris of useful and very useful diagnostic tests in rheumatology.
format	article
author	E. V. Kunder M. V. Volkova I. I. Generalov G. A. Nevinsky
author_facet	E. V. Kunder M. V. Volkova I. I. Generalov G. A. Nevinsky
author_sort	E. V. Kunder
title	CLINICAL AND DIAGNOSTIC SIGNIFICANCE OF DEOXYRIBONUCLEASE ACTIVITY OF POLYCLONAL IgG AND BLOOD SERA IN PATIENTS WITH SPONDYLOARTHRITIS
title_short	CLINICAL AND DIAGNOSTIC SIGNIFICANCE OF DEOXYRIBONUCLEASE ACTIVITY OF POLYCLONAL IgG AND BLOOD SERA IN PATIENTS WITH SPONDYLOARTHRITIS
title_full	CLINICAL AND DIAGNOSTIC SIGNIFICANCE OF DEOXYRIBONUCLEASE ACTIVITY OF POLYCLONAL IgG AND BLOOD SERA IN PATIENTS WITH SPONDYLOARTHRITIS
title_fullStr	CLINICAL AND DIAGNOSTIC SIGNIFICANCE OF DEOXYRIBONUCLEASE ACTIVITY OF POLYCLONAL IgG AND BLOOD SERA IN PATIENTS WITH SPONDYLOARTHRITIS
title_full_unstemmed	CLINICAL AND DIAGNOSTIC SIGNIFICANCE OF DEOXYRIBONUCLEASE ACTIVITY OF POLYCLONAL IgG AND BLOOD SERA IN PATIENTS WITH SPONDYLOARTHRITIS
title_sort	clinical and diagnostic significance of deoxyribonuclease activity of polyclonal igg and blood sera in patients with spondyloarthritis
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/caad527e08484104bc691fd6822689ac
work_keys_str_mv	AT evkunder clinicalanddiagnosticsignificanceofdeoxyribonucleaseactivityofpolyclonaliggandbloodserainpatientswithspondyloarthritis AT mvvolkova clinicalanddiagnosticsignificanceofdeoxyribonucleaseactivityofpolyclonaliggandbloodserainpatientswithspondyloarthritis AT iigeneralov clinicalanddiagnosticsignificanceofdeoxyribonucleaseactivityofpolyclonaliggandbloodserainpatientswithspondyloarthritis AT ganevinsky clinicalanddiagnosticsignificanceofdeoxyribonucleaseactivityofpolyclonaliggandbloodserainpatientswithspondyloarthritis
_version_	1718422508997181440

CLINICAL AND DIAGNOSTIC SIGNIFICANCE OF DEOXYRIBONUCLEASE ACTIVITY OF POLYCLONAL IgG AND BLOOD SERA IN PATIENTS WITH SPONDYLOARTHRITIS

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