Cassava cell wall characterization and degradation by a multicomponent NSP-targeting enzyme (NSPase)

Abstract Cassava (Manihot esculenta Crantz) is considered the third most important source of calories in tropical regions. Up to one third of cassava harvested worldwide is used in livestock production. The focus of this study was to characterize cassava cell wall structure to provide knowledge for...

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Autores principales:	Larissa Staack, Eduardo Antonio Della Pia, Bodil Jørgensen, Dan Pettersson, Ninfa Rangel Pedersen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cab15b11e23043568344a14f738d0cba
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spelling	oai:doaj.org-article:cab15b11e23043568344a14f738d0cba2021-12-02T15:09:00ZCassava cell wall characterization and degradation by a multicomponent NSP-targeting enzyme (NSPase)10.1038/s41598-019-46341-22045-2322https://doaj.org/article/cab15b11e23043568344a14f738d0cba2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-46341-2https://doaj.org/toc/2045-2322Abstract Cassava (Manihot esculenta Crantz) is considered the third most important source of calories in tropical regions. Up to one third of cassava harvested worldwide is used in livestock production. The focus of this study was to characterize cassava cell wall structure to provide knowledge for a better application of cassava as an energy source in monogastric animal feed. A total of five cassava samples from different feed mills in South East Asia were investigated. On a dry matter basis, the cassava cell walls contained, on average, 640 mg g−1 glucose, 140 mg g−1 galactose, 50 mg g−1 mannose, 80 mg g−1 xylose, 60 mg g−1 arabinose, 10 mg g−1 fucose and 20 mg g−1 rhamnose. RONOZYME VP (DSM Nutritional Products, Switzerland), a non-specific multicomponent non-starch polysaccharide (NSP) degrading enzyme (NSPase) product from Aspergillus aculeatus, solubilized about 10% of cassava NSP content during 4 h incubations at 40 °C and pH 5. There was notable solubilization of polymers containing uronic acids, galactose, arabinose and rhamnose. Immuno-microscopy imaging indicated the solubilization of pectin, galactan and xyloglucan polysaccharides from cassava cell wall. As a consequence, the starch granules became more available to exogenous α-amylase degradation.Larissa StaackEduardo Antonio Della PiaBodil JørgensenDan PetterssonNinfa Rangel PedersenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Larissa Staack Eduardo Antonio Della Pia Bodil Jørgensen Dan Pettersson Ninfa Rangel Pedersen Cassava cell wall characterization and degradation by a multicomponent NSP-targeting enzyme (NSPase)
description	Abstract Cassava (Manihot esculenta Crantz) is considered the third most important source of calories in tropical regions. Up to one third of cassava harvested worldwide is used in livestock production. The focus of this study was to characterize cassava cell wall structure to provide knowledge for a better application of cassava as an energy source in monogastric animal feed. A total of five cassava samples from different feed mills in South East Asia were investigated. On a dry matter basis, the cassava cell walls contained, on average, 640 mg g−1 glucose, 140 mg g−1 galactose, 50 mg g−1 mannose, 80 mg g−1 xylose, 60 mg g−1 arabinose, 10 mg g−1 fucose and 20 mg g−1 rhamnose. RONOZYME VP (DSM Nutritional Products, Switzerland), a non-specific multicomponent non-starch polysaccharide (NSP) degrading enzyme (NSPase) product from Aspergillus aculeatus, solubilized about 10% of cassava NSP content during 4 h incubations at 40 °C and pH 5. There was notable solubilization of polymers containing uronic acids, galactose, arabinose and rhamnose. Immuno-microscopy imaging indicated the solubilization of pectin, galactan and xyloglucan polysaccharides from cassava cell wall. As a consequence, the starch granules became more available to exogenous α-amylase degradation.
format	article
author	Larissa Staack Eduardo Antonio Della Pia Bodil Jørgensen Dan Pettersson Ninfa Rangel Pedersen
author_facet	Larissa Staack Eduardo Antonio Della Pia Bodil Jørgensen Dan Pettersson Ninfa Rangel Pedersen
author_sort	Larissa Staack
title	Cassava cell wall characterization and degradation by a multicomponent NSP-targeting enzyme (NSPase)
title_short	Cassava cell wall characterization and degradation by a multicomponent NSP-targeting enzyme (NSPase)
title_full	Cassava cell wall characterization and degradation by a multicomponent NSP-targeting enzyme (NSPase)
title_fullStr	Cassava cell wall characterization and degradation by a multicomponent NSP-targeting enzyme (NSPase)
title_full_unstemmed	Cassava cell wall characterization and degradation by a multicomponent NSP-targeting enzyme (NSPase)
title_sort	cassava cell wall characterization and degradation by a multicomponent nsp-targeting enzyme (nspase)
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/cab15b11e23043568344a14f738d0cba
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Cassava cell wall characterization and degradation by a multicomponent NSP-targeting enzyme (NSPase)

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