High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases

The development of site-specific recombinases as genome editing tools is limited by the difficulty of altering their DNA sequence specificity. Here the authors present Rec-seq, a method for identifying specificity determinants and off-target substrates of recombinases in an unbiased manner.

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Autores principales:	Jeffrey L. Bessen, Lena K. Afeyan, Vlado Dančík, Luke W. Koblan, David B. Thompson, Chas Leichner, Paul A. Clemons, David R. Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/cab979859ad4417b8ff4c6496b54b8cd
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spelling	oai:doaj.org-article:cab979859ad4417b8ff4c6496b54b8cd2021-12-02T15:35:08ZHigh-resolution specificity profiling and off-target prediction for site-specific DNA recombinases10.1038/s41467-019-09987-02041-1723https://doaj.org/article/cab979859ad4417b8ff4c6496b54b8cd2019-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09987-0https://doaj.org/toc/2041-1723The development of site-specific recombinases as genome editing tools is limited by the difficulty of altering their DNA sequence specificity. Here the authors present Rec-seq, a method for identifying specificity determinants and off-target substrates of recombinases in an unbiased manner.Jeffrey L. BessenLena K. AfeyanVlado DančíkLuke W. KoblanDavid B. ThompsonChas LeichnerPaul A. ClemonsDavid R. LiuNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jeffrey L. Bessen Lena K. Afeyan Vlado Dančík Luke W. Koblan David B. Thompson Chas Leichner Paul A. Clemons David R. Liu High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases
description	The development of site-specific recombinases as genome editing tools is limited by the difficulty of altering their DNA sequence specificity. Here the authors present Rec-seq, a method for identifying specificity determinants and off-target substrates of recombinases in an unbiased manner.
format	article
author	Jeffrey L. Bessen Lena K. Afeyan Vlado Dančík Luke W. Koblan David B. Thompson Chas Leichner Paul A. Clemons David R. Liu
author_facet	Jeffrey L. Bessen Lena K. Afeyan Vlado Dančík Luke W. Koblan David B. Thompson Chas Leichner Paul A. Clemons David R. Liu
author_sort	Jeffrey L. Bessen
title	High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases
title_short	High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases
title_full	High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases
title_fullStr	High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases
title_full_unstemmed	High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases
title_sort	high-resolution specificity profiling and off-target prediction for site-specific dna recombinases
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/cab979859ad4417b8ff4c6496b54b8cd
work_keys_str_mv	AT jeffreylbessen highresolutionspecificityprofilingandofftargetpredictionforsitespecificdnarecombinases AT lenakafeyan highresolutionspecificityprofilingandofftargetpredictionforsitespecificdnarecombinases AT vladodancik highresolutionspecificityprofilingandofftargetpredictionforsitespecificdnarecombinases AT lukewkoblan highresolutionspecificityprofilingandofftargetpredictionforsitespecificdnarecombinases AT davidbthompson highresolutionspecificityprofilingandofftargetpredictionforsitespecificdnarecombinases AT chasleichner highresolutionspecificityprofilingandofftargetpredictionforsitespecificdnarecombinases AT paulaclemons highresolutionspecificityprofilingandofftargetpredictionforsitespecificdnarecombinases AT davidrliu highresolutionspecificityprofilingandofftargetpredictionforsitespecificdnarecombinases
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High-resolution specificity profiling and off-target prediction for site-specific DNA recombinases

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