Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development.

Intracellular composition and the distribution of bio-molecules play central roles in the specification of cell fates and morphogenesis during embryogenesis. Consequently, investigation of changes in the expression and distribution of bio-molecules, especially mRNAs and proteins, is an important cha...

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Autores principales:	Mitsuru J Nakamura, Kohji Hotta, Kotaro Oka
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cac2801dbf024287b36108ecc7dc0019
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spelling	oai:doaj.org-article:cac2801dbf024287b36108ecc7dc00192021-11-18T08:58:49ZRaman spectroscopic imaging of the whole Ciona intestinalis embryo during development.1932-620310.1371/journal.pone.0071739https://doaj.org/article/cac2801dbf024287b36108ecc7dc00192013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23977129/?tool=EBIhttps://doaj.org/toc/1932-6203Intracellular composition and the distribution of bio-molecules play central roles in the specification of cell fates and morphogenesis during embryogenesis. Consequently, investigation of changes in the expression and distribution of bio-molecules, especially mRNAs and proteins, is an important challenge in developmental biology. Raman spectroscopic imaging, a non-invasive and label-free technique, allows simultaneous imaging of the intracellular composition and distribution of multiple bio-molecules. In this study, we explored the application of Raman spectroscopic imaging in the whole Ciona intestinalis embryo during development. Analysis of Raman spectra scattered from C. intestinalis embryos revealed a number of localized patterns of high Raman intensity within the embryo. Based on the observed distribution of bio-molecules, we succeeded in identifying the location and structure of differentiated muscle and endoderm within the whole embryo, up to the tailbud stage, in a label-free manner. Furthermore, during cell differentiation, we detected significant differences in cell state between muscle/endoderm daughter cells and daughter cells with other fates that had divided from the same mother cells; this was achieved by focusing on the Raman intensity of single Raman bands at 1002 or 1526 cm(-1), respectively. This study reports the first application of Raman spectroscopic imaging to the study of identifying and characterizing differentiating tissues in a whole chordate embryo. Our results suggest that Raman spectroscopic imaging is a feasible label-free technique for investigating the developmental process of the whole embryo of C. intestinalis.Mitsuru J NakamuraKohji HottaKotaro OkaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e71739 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mitsuru J Nakamura Kohji Hotta Kotaro Oka Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development.
description	Intracellular composition and the distribution of bio-molecules play central roles in the specification of cell fates and morphogenesis during embryogenesis. Consequently, investigation of changes in the expression and distribution of bio-molecules, especially mRNAs and proteins, is an important challenge in developmental biology. Raman spectroscopic imaging, a non-invasive and label-free technique, allows simultaneous imaging of the intracellular composition and distribution of multiple bio-molecules. In this study, we explored the application of Raman spectroscopic imaging in the whole Ciona intestinalis embryo during development. Analysis of Raman spectra scattered from C. intestinalis embryos revealed a number of localized patterns of high Raman intensity within the embryo. Based on the observed distribution of bio-molecules, we succeeded in identifying the location and structure of differentiated muscle and endoderm within the whole embryo, up to the tailbud stage, in a label-free manner. Furthermore, during cell differentiation, we detected significant differences in cell state between muscle/endoderm daughter cells and daughter cells with other fates that had divided from the same mother cells; this was achieved by focusing on the Raman intensity of single Raman bands at 1002 or 1526 cm(-1), respectively. This study reports the first application of Raman spectroscopic imaging to the study of identifying and characterizing differentiating tissues in a whole chordate embryo. Our results suggest that Raman spectroscopic imaging is a feasible label-free technique for investigating the developmental process of the whole embryo of C. intestinalis.
format	article
author	Mitsuru J Nakamura Kohji Hotta Kotaro Oka
author_facet	Mitsuru J Nakamura Kohji Hotta Kotaro Oka
author_sort	Mitsuru J Nakamura
title	Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development.
title_short	Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development.
title_full	Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development.
title_fullStr	Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development.
title_full_unstemmed	Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development.
title_sort	raman spectroscopic imaging of the whole ciona intestinalis embryo during development.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/cac2801dbf024287b36108ecc7dc0019
work_keys_str_mv	AT mitsurujnakamura ramanspectroscopicimagingofthewholecionaintestinalisembryoduringdevelopment AT kohjihotta ramanspectroscopicimagingofthewholecionaintestinalisembryoduringdevelopment AT kotarooka ramanspectroscopicimagingofthewholecionaintestinalisembryoduringdevelopment
_version_	1718421110048948224

Raman spectroscopic imaging of the whole Ciona intestinalis embryo during development.

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