Biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells

Antje Lindemann,1 Kerstin Lüdtke-Buzug,2 Bianca M Fräderich,1 Ksenija Gräfe,2 Ralph Pries,1 Barbara Wollenberg11Department of Otorhinolaryngology, University Hospital of Schleswig-Holstein, Luebeck, Germany; 2Institute of Medical Engineering, University of Luebeck, Luebec...

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Autores principales:	Lindemann A, Lüdtke-Buzug K, Fräderich BM, Gräfe K, Pries R, Wollenberg B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/cad0c2ef3e3d4d3f9c4d20c8fb344391
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spelling	oai:doaj.org-article:cad0c2ef3e3d4d3f9c4d20c8fb3443912021-12-02T01:01:50ZBiological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells1178-2013https://doaj.org/article/cad0c2ef3e3d4d3f9c4d20c8fb3443912014-10-01T00:00:00Zhttp://www.dovepress.com/biological-impact-of-superparamagnetic-iron-oxide-nanoparticles-for-ma-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Antje Lindemann,1 Kerstin Lüdtke-Buzug,2 Bianca M Fräderich,1 Ksenija Gräfe,2 Ralph Pries,1 Barbara Wollenberg11Department of Otorhinolaryngology, University Hospital of Schleswig-Holstein, Luebeck, Germany; 2Institute of Medical Engineering, University of Luebeck, Luebeck, GermanyBackground: As a tomographic imaging technology, magnetic particle imaging (MPI) allows high spatial resolution and sensitivity, and the possibility to create real-time images by determining the spatial distribution of magnetic particles. To ensure a prospective biosafe application of UL-D (University of Luebeck-Dextran coated superparamagnetic nanoparticles), we evaluated the biocompatibility of superparamagnetic iron oxide nanoparticles (SPIONs), their impact on biological properties, and their cellular uptake using head and neck squamous cancer cells (HNSCCs).Methods: SPIONs that met specific MPI requirements were synthesized as tracers. Labeling and uptake efficiency were analyzed by hematoxylin and eosin staining and magnetic particle spectrometry. Flow cytometry, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays, and real-time cell analyzer assays were used to investigate apoptosis, proliferation, and the cytokine response of SPION-labeled cells. The production of reactive oxygen species (ROS) was determined using a fluorescent dye. Experimental results were compared to the contrast agent Resovist®, a standard agent used in MPI.Results: UL-D nanoparticles and Resovist particles were taken up in vitro by HNSCCs via unspecific phagocytosis followed by cytosolic accumulation. To evaluate toxicity, flow cytometry analysis was performed; results showed that dose- and time-dependent administration of Resovist induced apoptosis whereas cell viability of UL-D-labeled cells was not altered. We observed decreased cell proliferation in response to increased SPION concentrations. An intracellular production of ROS could not be detected, suggesting that the particles did not cause oxidative stress. Tumor necrosis factor alpha (TNF-α) and interleukins IL-6, IL-8, and IL-1ß were measured to distinguish inflammatory responses. Only the primary tumor cell line labeled with >0.5 mM Resovist showed a significant increase in IL-1ß secretion.Conclusion: Our data suggest that UL-D SPIONs are a promising tracer material for use in innovative tumor cell analysis in MPI.Keywords: cell labeling, HNSCCs, biomedical imaging, iron oxide nanoparticles, cytotoxicityLindemann ALüdtke-Buzug KFräderich BMGräfe KPries RWollenberg BDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 5025-5040 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Lindemann A Lüdtke-Buzug K Fräderich BM Gräfe K Pries R Wollenberg B Biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells
description	Antje Lindemann,1 Kerstin Lüdtke-Buzug,2 Bianca M Fräderich,1 Ksenija Gräfe,2 Ralph Pries,1 Barbara Wollenberg11Department of Otorhinolaryngology, University Hospital of Schleswig-Holstein, Luebeck, Germany; 2Institute of Medical Engineering, University of Luebeck, Luebeck, GermanyBackground: As a tomographic imaging technology, magnetic particle imaging (MPI) allows high spatial resolution and sensitivity, and the possibility to create real-time images by determining the spatial distribution of magnetic particles. To ensure a prospective biosafe application of UL-D (University of Luebeck-Dextran coated superparamagnetic nanoparticles), we evaluated the biocompatibility of superparamagnetic iron oxide nanoparticles (SPIONs), their impact on biological properties, and their cellular uptake using head and neck squamous cancer cells (HNSCCs).Methods: SPIONs that met specific MPI requirements were synthesized as tracers. Labeling and uptake efficiency were analyzed by hematoxylin and eosin staining and magnetic particle spectrometry. Flow cytometry, 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays, and real-time cell analyzer assays were used to investigate apoptosis, proliferation, and the cytokine response of SPION-labeled cells. The production of reactive oxygen species (ROS) was determined using a fluorescent dye. Experimental results were compared to the contrast agent Resovist®, a standard agent used in MPI.Results: UL-D nanoparticles and Resovist particles were taken up in vitro by HNSCCs via unspecific phagocytosis followed by cytosolic accumulation. To evaluate toxicity, flow cytometry analysis was performed; results showed that dose- and time-dependent administration of Resovist induced apoptosis whereas cell viability of UL-D-labeled cells was not altered. We observed decreased cell proliferation in response to increased SPION concentrations. An intracellular production of ROS could not be detected, suggesting that the particles did not cause oxidative stress. Tumor necrosis factor alpha (TNF-α) and interleukins IL-6, IL-8, and IL-1ß were measured to distinguish inflammatory responses. Only the primary tumor cell line labeled with >0.5 mM Resovist showed a significant increase in IL-1ß secretion.Conclusion: Our data suggest that UL-D SPIONs are a promising tracer material for use in innovative tumor cell analysis in MPI.Keywords: cell labeling, HNSCCs, biomedical imaging, iron oxide nanoparticles, cytotoxicity
format	article
author	Lindemann A Lüdtke-Buzug K Fräderich BM Gräfe K Pries R Wollenberg B
author_facet	Lindemann A Lüdtke-Buzug K Fräderich BM Gräfe K Pries R Wollenberg B
author_sort	Lindemann A
title	Biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells
title_short	Biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells
title_full	Biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells
title_fullStr	Biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells
title_full_unstemmed	Biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells
title_sort	biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/cad0c2ef3e3d4d3f9c4d20c8fb344391
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Biological impact of superparamagnetic iron oxide nanoparticles for magnetic particle imaging of head and neck cancer cells

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