Transcriptome-based analysis of resistance mechanism to black point caused by Bipolaris sorokiniana in wheat

Abstract Black point is a cereal disease caused by complex pathogens, of which the pathogenicity of Bipolaris sorokiniana is the most serious in wheat. Resistance to black point is quantitative in nature, and thus the mechanism is poorly understood. We conducted a comparative transcriptome analysis...

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Autores principales:	Qiaoyun Li, Chuang Gao, Kaige Xu, Yumei Jiang, Jishan Niu, Guihong Yin, Chenyang Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cad41052d5164a828e595320377c8def
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spelling	oai:doaj.org-article:cad41052d5164a828e595320377c8def2021-12-02T17:04:34ZTranscriptome-based analysis of resistance mechanism to black point caused by Bipolaris sorokiniana in wheat10.1038/s41598-021-86303-12045-2322https://doaj.org/article/cad41052d5164a828e595320377c8def2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86303-1https://doaj.org/toc/2045-2322Abstract Black point is a cereal disease caused by complex pathogens, of which the pathogenicity of Bipolaris sorokiniana is the most serious in wheat. Resistance to black point is quantitative in nature, and thus the mechanism is poorly understood. We conducted a comparative transcriptome analysis to identify differentially expressed genes (DEGs) in black point-slightly susceptible and -highly susceptible wheat lines at different timepoints following B. sorokiniana inoculation. DEGs associated with photosynthesis were upregulated in black point-slightly susceptible lines. The top Gene Ontology enrichment terms for biological processes were oxidation–reduction, response to cold, salt stress, oxidative stress, and cadmium ion; terms for cellular component genes were mainly involved in plasma membrane and cytoplasmic membrane-bounded vesicle, whereas those for molecular function were heme binding and peroxidase activity. Moreover, activities of antioxidant enzymes superoxide dismutase, catalase, and peroxidase were higher in slightly susceptible lines than those in highly susceptible lines (except peroxidase 12–24 days post-inoculation). Thus, resistance to B. sorokiniana-caused black point in wheat was mainly related to counteracting oxidative stress, although the specific metabolic pathways require further study. This study presents new insights for understanding resistance mechanisms of selected wheat lines to black point.Qiaoyun LiChuang GaoKaige XuYumei JiangJishan NiuGuihong YinChenyang WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qiaoyun Li Chuang Gao Kaige Xu Yumei Jiang Jishan Niu Guihong Yin Chenyang Wang Transcriptome-based analysis of resistance mechanism to black point caused by Bipolaris sorokiniana in wheat
description	Abstract Black point is a cereal disease caused by complex pathogens, of which the pathogenicity of Bipolaris sorokiniana is the most serious in wheat. Resistance to black point is quantitative in nature, and thus the mechanism is poorly understood. We conducted a comparative transcriptome analysis to identify differentially expressed genes (DEGs) in black point-slightly susceptible and -highly susceptible wheat lines at different timepoints following B. sorokiniana inoculation. DEGs associated with photosynthesis were upregulated in black point-slightly susceptible lines. The top Gene Ontology enrichment terms for biological processes were oxidation–reduction, response to cold, salt stress, oxidative stress, and cadmium ion; terms for cellular component genes were mainly involved in plasma membrane and cytoplasmic membrane-bounded vesicle, whereas those for molecular function were heme binding and peroxidase activity. Moreover, activities of antioxidant enzymes superoxide dismutase, catalase, and peroxidase were higher in slightly susceptible lines than those in highly susceptible lines (except peroxidase 12–24 days post-inoculation). Thus, resistance to B. sorokiniana-caused black point in wheat was mainly related to counteracting oxidative stress, although the specific metabolic pathways require further study. This study presents new insights for understanding resistance mechanisms of selected wheat lines to black point.
format	article
author	Qiaoyun Li Chuang Gao Kaige Xu Yumei Jiang Jishan Niu Guihong Yin Chenyang Wang
author_facet	Qiaoyun Li Chuang Gao Kaige Xu Yumei Jiang Jishan Niu Guihong Yin Chenyang Wang
author_sort	Qiaoyun Li
title	Transcriptome-based analysis of resistance mechanism to black point caused by Bipolaris sorokiniana in wheat
title_short	Transcriptome-based analysis of resistance mechanism to black point caused by Bipolaris sorokiniana in wheat
title_full	Transcriptome-based analysis of resistance mechanism to black point caused by Bipolaris sorokiniana in wheat
title_fullStr	Transcriptome-based analysis of resistance mechanism to black point caused by Bipolaris sorokiniana in wheat
title_full_unstemmed	Transcriptome-based analysis of resistance mechanism to black point caused by Bipolaris sorokiniana in wheat
title_sort	transcriptome-based analysis of resistance mechanism to black point caused by bipolaris sorokiniana in wheat
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cad41052d5164a828e595320377c8def
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_version_	1718381875683131392

Transcriptome-based analysis of resistance mechanism to black point caused by Bipolaris sorokiniana in wheat

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