Blood donor variability is a modulatory factor for P. falciparum invasion phenotyping assays

Abstract Human erythrocytes are indispensable for Plasmodium falciparum development. Unlike other eukaryotic cells, there is no existing erythroid cell line capable of supporting long-term P. falciparum in vitro experiments. Consequently, invasion phenotyping experiments rely on erythrocytes of diff...

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Autores principales:	Laty G. Thiam, Prince B. Nyarko, Kwadwo A. Kusi, Makhtar Niang, Yaw Aniweh, Gordon A. Awandare
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cae7a8e92e654596bfc0b1d9283e9146
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spelling	oai:doaj.org-article:cae7a8e92e654596bfc0b1d9283e91462021-12-02T14:25:16ZBlood donor variability is a modulatory factor for P. falciparum invasion phenotyping assays10.1038/s41598-021-86438-12045-2322https://doaj.org/article/cae7a8e92e654596bfc0b1d9283e91462021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86438-1https://doaj.org/toc/2045-2322Abstract Human erythrocytes are indispensable for Plasmodium falciparum development. Unlike other eukaryotic cells, there is no existing erythroid cell line capable of supporting long-term P. falciparum in vitro experiments. Consequently, invasion phenotyping experiments rely on erythrocytes of different individuals. However, the contribution of the erythrocytes variation in influencing invasion rates remains unknown, which represents a challenge for conducting large-scale comparative studies. Here, we used erythrocytes of different blood groups harboring different hemoglobin genotypes to assess the relative contribution of blood donor variability in P. falciparum invasion phenotyping assays. For each donor, we investigated the relationship between parasite invasion phenotypes and erythrocyte phenotypic characteristics, including the expression levels of surface receptors (e.g. the human glycophorins A and C, the complement receptor 1 and decay accelerating factor), blood groups (e.g. ABO/Rh system), and hemoglobin genotypes (e.g. AA, AS and AC). Across all donors, there were significant differences in invasion efficiency following treatment with either neuraminidase, trypsin or chymotrypsin relative to the control erythrocytes. Primarily, we showed that the levels of key erythrocyte surface receptors and their sensitivity to enzyme treatment significantly differed across donors. However, invasion efficiency did not correlate with susceptibility to enzyme treatment or with the levels of the selected erythrocyte surface receptors. Furthermore, we found no relationship between P. falciparum invasion phenotype and blood group or hemoglobin genotype. Altogether, our findings demonstrate the need to consider erythrocyte donor uniformity and anticipate challenges associated with blood donor variability in early stages of large-scale study design.Laty G. ThiamPrince B. NyarkoKwadwo A. KusiMakhtar NiangYaw AniwehGordon A. AwandareNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Laty G. Thiam Prince B. Nyarko Kwadwo A. Kusi Makhtar Niang Yaw Aniweh Gordon A. Awandare Blood donor variability is a modulatory factor for P. falciparum invasion phenotyping assays
description	Abstract Human erythrocytes are indispensable for Plasmodium falciparum development. Unlike other eukaryotic cells, there is no existing erythroid cell line capable of supporting long-term P. falciparum in vitro experiments. Consequently, invasion phenotyping experiments rely on erythrocytes of different individuals. However, the contribution of the erythrocytes variation in influencing invasion rates remains unknown, which represents a challenge for conducting large-scale comparative studies. Here, we used erythrocytes of different blood groups harboring different hemoglobin genotypes to assess the relative contribution of blood donor variability in P. falciparum invasion phenotyping assays. For each donor, we investigated the relationship between parasite invasion phenotypes and erythrocyte phenotypic characteristics, including the expression levels of surface receptors (e.g. the human glycophorins A and C, the complement receptor 1 and decay accelerating factor), blood groups (e.g. ABO/Rh system), and hemoglobin genotypes (e.g. AA, AS and AC). Across all donors, there were significant differences in invasion efficiency following treatment with either neuraminidase, trypsin or chymotrypsin relative to the control erythrocytes. Primarily, we showed that the levels of key erythrocyte surface receptors and their sensitivity to enzyme treatment significantly differed across donors. However, invasion efficiency did not correlate with susceptibility to enzyme treatment or with the levels of the selected erythrocyte surface receptors. Furthermore, we found no relationship between P. falciparum invasion phenotype and blood group or hemoglobin genotype. Altogether, our findings demonstrate the need to consider erythrocyte donor uniformity and anticipate challenges associated with blood donor variability in early stages of large-scale study design.
format	article
author	Laty G. Thiam Prince B. Nyarko Kwadwo A. Kusi Makhtar Niang Yaw Aniweh Gordon A. Awandare
author_facet	Laty G. Thiam Prince B. Nyarko Kwadwo A. Kusi Makhtar Niang Yaw Aniweh Gordon A. Awandare
author_sort	Laty G. Thiam
title	Blood donor variability is a modulatory factor for P. falciparum invasion phenotyping assays
title_short	Blood donor variability is a modulatory factor for P. falciparum invasion phenotyping assays
title_full	Blood donor variability is a modulatory factor for P. falciparum invasion phenotyping assays
title_fullStr	Blood donor variability is a modulatory factor for P. falciparum invasion phenotyping assays
title_full_unstemmed	Blood donor variability is a modulatory factor for P. falciparum invasion phenotyping assays
title_sort	blood donor variability is a modulatory factor for p. falciparum invasion phenotyping assays
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cae7a8e92e654596bfc0b1d9283e9146
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Blood donor variability is a modulatory factor for P. falciparum invasion phenotyping assays

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