Chronic fluoxetine treatment increases daytime melatonin synthesis in the rodent

Gillian W Reierson, Claudio A Mastronardi, Julio Licinio, Ma-Li WongCenter on Pharmacogenomics, Department of Psychiatry and Behavioral Sciences, University of Miami Miller School of Medicine, Miami, FL, USAAbstract: Circadian rhythm disturbances can occur as part of the clinical symptoms of major d...

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Autores principales:	Gillian W Reierson, Claudio A Mastronardi, Julio Licinio, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2009
Materias:	Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/caeb08e51ddb44cba701f8a004d22c7a
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spelling	oai:doaj.org-article:caeb08e51ddb44cba701f8a004d22c7a2021-12-02T04:21:37ZChronic fluoxetine treatment increases daytime melatonin synthesis in the rodent1179-1438https://doaj.org/article/caeb08e51ddb44cba701f8a004d22c7a2009-09-01T00:00:00Zhttp://www.dovepress.com/chronic-fluoxetine-treatment-increases-daytime-melatonin-synthesis-in--a3520https://doaj.org/toc/1179-1438Gillian W Reierson, Claudio A Mastronardi, Julio Licinio, Ma-Li WongCenter on Pharmacogenomics, Department of Psychiatry and Behavioral Sciences, University of Miami Miller School of Medicine, Miami, FL, USAAbstract: Circadian rhythm disturbances can occur as part of the clinical symptoms of major depressive disorder and have been found to resolve with antidepressant therapy. The pineal gland is relevant to circadian rhythms as it secretes the hormone melatonin following activation of the cyclic adenosine monophosphate (cAMP) signaling cascade and of arylalkylamine N-acetyltransferase (AA-NAT), the rate-limiting enzyme for its synthesis. Cyclic AMP is synthesized by adenylate cyclases (AC) and degraded by phosphodiesterases (PDEs). Little is known about the contribution of the PDE system to antidepressant-induced alterations in pineal cAMP signaling and melatonin synthesis. In the present study we used enzyme immunoassay to measure plasma melatonin levels and pineal cAMP levels and as well as quantitative real-time polymerase chain reaction to measure pineal expression of PDE, AC, and AA-NAT genes in rats chronically treated with the prototypic antidepressant fluoxetine. We found elevated melatonin synthesis with increased pineal AA-NAT gene expression and daytime plasma melatonin levels and downregulated cAMP signaling with increased PDE and unchanged AC pineal gene expression, and decreased content of pineal cAMP. We conclude that chronic fluoxetine treatment increases daytime plasma melatonin and pineal AA-NAT gene expression despite downregulated pineal cAMP signaling in the rodent.Keywords: antidepressant, melatonin, pineal, nucleotides, cyclic, phosphodiesterase, rat Gillian W ReiersonClaudio A MastronardiJulio Licinioet alDove Medical PressarticleTherapeutics. PharmacologyRM1-950ENClinical Pharmacology: Advances and Applications, Vol 2009, Iss Default, Pp 1-6 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Therapeutics. Pharmacology RM1-950
spellingShingle	Therapeutics. Pharmacology RM1-950 Gillian W Reierson Claudio A Mastronardi Julio Licinio et al Chronic fluoxetine treatment increases daytime melatonin synthesis in the rodent
description	Gillian W Reierson, Claudio A Mastronardi, Julio Licinio, Ma-Li WongCenter on Pharmacogenomics, Department of Psychiatry and Behavioral Sciences, University of Miami Miller School of Medicine, Miami, FL, USAAbstract: Circadian rhythm disturbances can occur as part of the clinical symptoms of major depressive disorder and have been found to resolve with antidepressant therapy. The pineal gland is relevant to circadian rhythms as it secretes the hormone melatonin following activation of the cyclic adenosine monophosphate (cAMP) signaling cascade and of arylalkylamine N-acetyltransferase (AA-NAT), the rate-limiting enzyme for its synthesis. Cyclic AMP is synthesized by adenylate cyclases (AC) and degraded by phosphodiesterases (PDEs). Little is known about the contribution of the PDE system to antidepressant-induced alterations in pineal cAMP signaling and melatonin synthesis. In the present study we used enzyme immunoassay to measure plasma melatonin levels and pineal cAMP levels and as well as quantitative real-time polymerase chain reaction to measure pineal expression of PDE, AC, and AA-NAT genes in rats chronically treated with the prototypic antidepressant fluoxetine. We found elevated melatonin synthesis with increased pineal AA-NAT gene expression and daytime plasma melatonin levels and downregulated cAMP signaling with increased PDE and unchanged AC pineal gene expression, and decreased content of pineal cAMP. We conclude that chronic fluoxetine treatment increases daytime plasma melatonin and pineal AA-NAT gene expression despite downregulated pineal cAMP signaling in the rodent.Keywords: antidepressant, melatonin, pineal, nucleotides, cyclic, phosphodiesterase, rat
format	article
author	Gillian W Reierson Claudio A Mastronardi Julio Licinio et al
author_facet	Gillian W Reierson Claudio A Mastronardi Julio Licinio et al
author_sort	Gillian W Reierson
title	Chronic fluoxetine treatment increases daytime melatonin synthesis in the rodent
title_short	Chronic fluoxetine treatment increases daytime melatonin synthesis in the rodent
title_full	Chronic fluoxetine treatment increases daytime melatonin synthesis in the rodent
title_fullStr	Chronic fluoxetine treatment increases daytime melatonin synthesis in the rodent
title_full_unstemmed	Chronic fluoxetine treatment increases daytime melatonin synthesis in the rodent
title_sort	chronic fluoxetine treatment increases daytime melatonin synthesis in the rodent
publisher	Dove Medical Press
publishDate	2009
url	https://doaj.org/article/caeb08e51ddb44cba701f8a004d22c7a
work_keys_str_mv	AT gillianwreierson chronicfluoxetinetreatmentincreasesdaytimemelatoninsynthesisintherodent AT claudioamastronardi chronicfluoxetinetreatmentincreasesdaytimemelatoninsynthesisintherodent AT juliolicinio chronicfluoxetinetreatmentincreasesdaytimemelatoninsynthesisintherodent AT etal chronicfluoxetinetreatmentincreasesdaytimemelatoninsynthesisintherodent
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Chronic fluoxetine treatment increases daytime melatonin synthesis in the rodent

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