GLP-1 receptor signalling promotes β-cell glucose metabolism via mTOR-dependent HIF-1α activation

Abstract Glucagon-like peptide-1 (GLP-1) promotes insulin secretion from pancreatic β-cells in a glucose dependent manner. Several pathways mediate this action by rapid, kinase phosphorylation-dependent, but gene expression-independent mechanisms. Since GLP-1-induced insulin secretion requires gluco...

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Autores principales: Rodrigo Carlessi, Younan Chen, Jordan Rowlands, Vinicius F. Cruzat, Kevin N. Keane, Lauren Egan, Cyril Mamotte, Rebecca Stokes, Jenny E. Gunton, Paulo Ivo Homem de Bittencourt, Philip Newsholme
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Publicado: Nature Portfolio 2017
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spelling oai:doaj.org-article:caf5cdca187a473db8fc37928e03d4c92021-12-02T11:53:10ZGLP-1 receptor signalling promotes β-cell glucose metabolism via mTOR-dependent HIF-1α activation10.1038/s41598-017-02838-22045-2322https://doaj.org/article/caf5cdca187a473db8fc37928e03d4c92017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02838-2https://doaj.org/toc/2045-2322Abstract Glucagon-like peptide-1 (GLP-1) promotes insulin secretion from pancreatic β-cells in a glucose dependent manner. Several pathways mediate this action by rapid, kinase phosphorylation-dependent, but gene expression-independent mechanisms. Since GLP-1-induced insulin secretion requires glucose metabolism, we aimed to address the hypothesis that GLP-1 receptor (GLP-1R) signalling can modulate glucose uptake and utilization in β-cells. We have assessed various metabolic parameters after short and long exposure of clonal BRIN-BD11 β-cells and rodent islets to the GLP-1R agonist Exendin-4 (50 nM). Here we report for the first time that prolonged stimulation of the GLP-1R for 18 hours promotes metabolic reprogramming of β-cells. This is evidenced by up-regulation of glycolytic enzyme expression, increased rates of glucose uptake and consumption, as well as augmented ATP content, insulin secretion and glycolytic flux after removal of Exendin-4. In our model, depletion of Hypoxia-Inducible Factor 1 alpha (HIF-1α) impaired the effects of Exendin-4 on glucose metabolism, while pharmacological inhibition of Phosphoinositide 3-kinase (PI3K) or mTOR completely abolished such effects. Considering the central role of glucose catabolism for stimulus-secretion coupling in β-cells, our findings suggest that chronic GLP-1 actions on insulin secretion include elevated β-cell glucose metabolism. Moreover, our data reveal novel aspects of GLP-1 stimulated insulin secretion involving de novo gene expression.Rodrigo CarlessiYounan ChenJordan RowlandsVinicius F. CruzatKevin N. KeaneLauren EganCyril MamotteRebecca StokesJenny E. GuntonPaulo Ivo Homem de BittencourtPhilip NewsholmeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Rodrigo Carlessi
Younan Chen
Jordan Rowlands
Vinicius F. Cruzat
Kevin N. Keane
Lauren Egan
Cyril Mamotte
Rebecca Stokes
Jenny E. Gunton
Paulo Ivo Homem de Bittencourt
Philip Newsholme
GLP-1 receptor signalling promotes β-cell glucose metabolism via mTOR-dependent HIF-1α activation
description Abstract Glucagon-like peptide-1 (GLP-1) promotes insulin secretion from pancreatic β-cells in a glucose dependent manner. Several pathways mediate this action by rapid, kinase phosphorylation-dependent, but gene expression-independent mechanisms. Since GLP-1-induced insulin secretion requires glucose metabolism, we aimed to address the hypothesis that GLP-1 receptor (GLP-1R) signalling can modulate glucose uptake and utilization in β-cells. We have assessed various metabolic parameters after short and long exposure of clonal BRIN-BD11 β-cells and rodent islets to the GLP-1R agonist Exendin-4 (50 nM). Here we report for the first time that prolonged stimulation of the GLP-1R for 18 hours promotes metabolic reprogramming of β-cells. This is evidenced by up-regulation of glycolytic enzyme expression, increased rates of glucose uptake and consumption, as well as augmented ATP content, insulin secretion and glycolytic flux after removal of Exendin-4. In our model, depletion of Hypoxia-Inducible Factor 1 alpha (HIF-1α) impaired the effects of Exendin-4 on glucose metabolism, while pharmacological inhibition of Phosphoinositide 3-kinase (PI3K) or mTOR completely abolished such effects. Considering the central role of glucose catabolism for stimulus-secretion coupling in β-cells, our findings suggest that chronic GLP-1 actions on insulin secretion include elevated β-cell glucose metabolism. Moreover, our data reveal novel aspects of GLP-1 stimulated insulin secretion involving de novo gene expression.
format article
author Rodrigo Carlessi
Younan Chen
Jordan Rowlands
Vinicius F. Cruzat
Kevin N. Keane
Lauren Egan
Cyril Mamotte
Rebecca Stokes
Jenny E. Gunton
Paulo Ivo Homem de Bittencourt
Philip Newsholme
author_facet Rodrigo Carlessi
Younan Chen
Jordan Rowlands
Vinicius F. Cruzat
Kevin N. Keane
Lauren Egan
Cyril Mamotte
Rebecca Stokes
Jenny E. Gunton
Paulo Ivo Homem de Bittencourt
Philip Newsholme
author_sort Rodrigo Carlessi
title GLP-1 receptor signalling promotes β-cell glucose metabolism via mTOR-dependent HIF-1α activation
title_short GLP-1 receptor signalling promotes β-cell glucose metabolism via mTOR-dependent HIF-1α activation
title_full GLP-1 receptor signalling promotes β-cell glucose metabolism via mTOR-dependent HIF-1α activation
title_fullStr GLP-1 receptor signalling promotes β-cell glucose metabolism via mTOR-dependent HIF-1α activation
title_full_unstemmed GLP-1 receptor signalling promotes β-cell glucose metabolism via mTOR-dependent HIF-1α activation
title_sort glp-1 receptor signalling promotes β-cell glucose metabolism via mtor-dependent hif-1α activation
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/caf5cdca187a473db8fc37928e03d4c9
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