Analysis of the Serum Peptidomics Profile for Cats With Sarcomeric Gene Mutation and Hypertrophic Cardiomyopathy

Background: Hypertrophic cardiomyopathy (HCM) has a complex phenotype that is partly explained by genetic variants related to this disease. The serum peptidome profile is a promising approach to define clinically relevant biomarkers. This study aimed to classify peptide patterns in serum samples bet...

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Autores principales:	Pratch Sukumolanan, Narumon Phanakrop, Siriwan Thaisakun, Sittiruk Roytrakul, Soontaree Petchdee
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	feline hypertrophic cardiomyopathy myocardial disease peptidomics analysis echocardiography Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/caf76add5599445c9a1117360825a97b
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spelling	oai:doaj.org-article:caf76add5599445c9a1117360825a97b2021-11-08T05:15:53ZAnalysis of the Serum Peptidomics Profile for Cats With Sarcomeric Gene Mutation and Hypertrophic Cardiomyopathy2297-176910.3389/fvets.2021.771408https://doaj.org/article/caf76add5599445c9a1117360825a97b2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.771408/fullhttps://doaj.org/toc/2297-1769Background: Hypertrophic cardiomyopathy (HCM) has a complex phenotype that is partly explained by genetic variants related to this disease. The serum peptidome profile is a promising approach to define clinically relevant biomarkers. This study aimed to classify peptide patterns in serum samples between cats with sarcomeric gene mutations and normal cats.Materials and Methods: In the total serum samples from 31 cats, several essential proteins were identified by peptidomics analysis. The 5,946 peptides were differentially expressed in cats with sarcomeric gene mutations compared with cats without mutations.Results: Our results demonstrated characteristic protein expression in control cats, Maine Coon cats, and Maine Coon cats with gene mutations. In cats with gene mutations, peptide expression profiling showed an association with three peptides, Cytochrome 3a132 (CYP3A132), forkhead box O1 (FOXO1), and ArfGAP, with GTPase domains, ankyrin repeats, and PH domain 2 (AGAP2).Discussion: The serum peptidome of cats with mutations might provide supporting evidence for the dysregulation of metabolic and structural proteins. Genetic and peptidomics investigations may help elucidate the phenotypic variability of HCM and treatment targets to reduce morbidity and mortality of HCM in cats.Pratch SukumolananNarumon PhanakropSiriwan ThaisakunSittiruk RoytrakulSoontaree PetchdeeFrontiers Media S.A.articlefelinehypertrophic cardiomyopathymyocardial diseasepeptidomics analysisechocardiographyVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	feline hypertrophic cardiomyopathy myocardial disease peptidomics analysis echocardiography Veterinary medicine SF600-1100
spellingShingle	feline hypertrophic cardiomyopathy myocardial disease peptidomics analysis echocardiography Veterinary medicine SF600-1100 Pratch Sukumolanan Narumon Phanakrop Siriwan Thaisakun Sittiruk Roytrakul Soontaree Petchdee Analysis of the Serum Peptidomics Profile for Cats With Sarcomeric Gene Mutation and Hypertrophic Cardiomyopathy
description	Background: Hypertrophic cardiomyopathy (HCM) has a complex phenotype that is partly explained by genetic variants related to this disease. The serum peptidome profile is a promising approach to define clinically relevant biomarkers. This study aimed to classify peptide patterns in serum samples between cats with sarcomeric gene mutations and normal cats.Materials and Methods: In the total serum samples from 31 cats, several essential proteins were identified by peptidomics analysis. The 5,946 peptides were differentially expressed in cats with sarcomeric gene mutations compared with cats without mutations.Results: Our results demonstrated characteristic protein expression in control cats, Maine Coon cats, and Maine Coon cats with gene mutations. In cats with gene mutations, peptide expression profiling showed an association with three peptides, Cytochrome 3a132 (CYP3A132), forkhead box O1 (FOXO1), and ArfGAP, with GTPase domains, ankyrin repeats, and PH domain 2 (AGAP2).Discussion: The serum peptidome of cats with mutations might provide supporting evidence for the dysregulation of metabolic and structural proteins. Genetic and peptidomics investigations may help elucidate the phenotypic variability of HCM and treatment targets to reduce morbidity and mortality of HCM in cats.
format	article
author	Pratch Sukumolanan Narumon Phanakrop Siriwan Thaisakun Sittiruk Roytrakul Soontaree Petchdee
author_facet	Pratch Sukumolanan Narumon Phanakrop Siriwan Thaisakun Sittiruk Roytrakul Soontaree Petchdee
author_sort	Pratch Sukumolanan
title	Analysis of the Serum Peptidomics Profile for Cats With Sarcomeric Gene Mutation and Hypertrophic Cardiomyopathy
title_short	Analysis of the Serum Peptidomics Profile for Cats With Sarcomeric Gene Mutation and Hypertrophic Cardiomyopathy
title_full	Analysis of the Serum Peptidomics Profile for Cats With Sarcomeric Gene Mutation and Hypertrophic Cardiomyopathy
title_fullStr	Analysis of the Serum Peptidomics Profile for Cats With Sarcomeric Gene Mutation and Hypertrophic Cardiomyopathy
title_full_unstemmed	Analysis of the Serum Peptidomics Profile for Cats With Sarcomeric Gene Mutation and Hypertrophic Cardiomyopathy
title_sort	analysis of the serum peptidomics profile for cats with sarcomeric gene mutation and hypertrophic cardiomyopathy
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/caf76add5599445c9a1117360825a97b
work_keys_str_mv	AT pratchsukumolanan analysisoftheserumpeptidomicsprofileforcatswithsarcomericgenemutationandhypertrophiccardiomyopathy AT narumonphanakrop analysisoftheserumpeptidomicsprofileforcatswithsarcomericgenemutationandhypertrophiccardiomyopathy AT siriwanthaisakun analysisoftheserumpeptidomicsprofileforcatswithsarcomericgenemutationandhypertrophiccardiomyopathy AT sittirukroytrakul analysisoftheserumpeptidomicsprofileforcatswithsarcomericgenemutationandhypertrophiccardiomyopathy AT soontareepetchdee analysisoftheserumpeptidomicsprofileforcatswithsarcomericgenemutationandhypertrophiccardiomyopathy
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Analysis of the Serum Peptidomics Profile for Cats With Sarcomeric Gene Mutation and Hypertrophic Cardiomyopathy

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