Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines

Samer Hasan Hussein Al Ali1, Mothanna Al-Qubaisi2, Mohd Zobir Hussein1,3, Zulkarnain Zainal1, Muhammad Nazrul Hakim41Department of Chemistry, Faculty of Science; 2Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Science; 3Advanced Materials and Nanotechnology Labor...

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Autores principales:	Hussein Al Ali SH, Al-Qubaisi M, Hussein MZ, Zainal Z, Hakim MN
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/caff7d07857141e49678b4483f8db0a0
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spelling	oai:doaj.org-article:caff7d07857141e49678b4483f8db0a02021-12-02T02:30:51ZPreparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines1176-91141178-2013https://doaj.org/article/caff7d07857141e49678b4483f8db0a02011-12-01T00:00:00Zhttp://www.dovepress.com/preparation-of-hippurate-zinc-layered-hydroxide-nanohybrid-and-its-syn-a8768https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Samer Hasan Hussein Al Ali1, Mothanna Al-Qubaisi2, Mohd Zobir Hussein1,3, Zulkarnain Zainal1, Muhammad Nazrul Hakim41Department of Chemistry, Faculty of Science; 2Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Science; 3Advanced Materials and Nanotechnology Laboratory, Institute of Advanced Technology; 4Department of Biomedical Science, Faculty of Medicine and Health Science, Universiti Putra Malaysia, Serdang, Selangor, MalaysiaBackground: A new simple preparation method for a hippurate-intercalated zinc-layered hydroxide (ZLH) nanohybrid has been established, which does not need an anion-exchange procedure to intercalate the hippurate anion into ZLH interlayers.Methods: The hippuric acid nanohybrid (HAN) was prepared by direct reaction of an aqueous suspension of zinc oxide with a solution of hippuric acid via a one-step method.Results: The basal spacing of the nanohybrid was 21.3 &Aring;, indicating that the hippurate anion was successfully intercalated into the interlayer space of ZLH, and arranged in a monolayer fashion with the carboxylate group pointing toward the ZLH inorganic interlayers. A Fourier transform infrared study confirmed the formation of the nanohybrid, while thermogravimetry and differential thermogravimetry analyses showed that the thermal stability of the nanohybrid was markedly enhanced. The loading of hippurate in the nanohybrid was estimated to be about 38.7% (w/w), and the release of hippurate from the nanohybrid was of a controlled manner, and therefore the resulting material was suitable for use as a controlled-release formulation. HAN has synergistic properties with tamoxifen toward a HepG2 cell line, with an IC50 value of 0.35 compared with hippurate. In the antiproliferative assay, the ratio of viable cells account for cells treated by the combination tamoxifen with HAN to untreated cells was sharply reduced from 66% to 13% after 24 and 72 hours, respectively.Conclusion: The release of hippuric acid anions from HAN occurred in a controlled manner, and the resulting material is suitable for a controlled-release formulation.Keywords: hippuric acid, nanohybrids, zinc oxide, zinc-layered hydroxide, synergistic effectHussein Al Ali SHAl-Qubaisi MHussein MZZainal ZHakim MNDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 3099-3111 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Hussein Al Ali SH Al-Qubaisi M Hussein MZ Zainal Z Hakim MN Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines
description	Samer Hasan Hussein Al Ali1, Mothanna Al-Qubaisi2, Mohd Zobir Hussein1,3, Zulkarnain Zainal1, Muhammad Nazrul Hakim41Department of Chemistry, Faculty of Science; 2Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Science; 3Advanced Materials and Nanotechnology Laboratory, Institute of Advanced Technology; 4Department of Biomedical Science, Faculty of Medicine and Health Science, Universiti Putra Malaysia, Serdang, Selangor, MalaysiaBackground: A new simple preparation method for a hippurate-intercalated zinc-layered hydroxide (ZLH) nanohybrid has been established, which does not need an anion-exchange procedure to intercalate the hippurate anion into ZLH interlayers.Methods: The hippuric acid nanohybrid (HAN) was prepared by direct reaction of an aqueous suspension of zinc oxide with a solution of hippuric acid via a one-step method.Results: The basal spacing of the nanohybrid was 21.3 &Aring;, indicating that the hippurate anion was successfully intercalated into the interlayer space of ZLH, and arranged in a monolayer fashion with the carboxylate group pointing toward the ZLH inorganic interlayers. A Fourier transform infrared study confirmed the formation of the nanohybrid, while thermogravimetry and differential thermogravimetry analyses showed that the thermal stability of the nanohybrid was markedly enhanced. The loading of hippurate in the nanohybrid was estimated to be about 38.7% (w/w), and the release of hippurate from the nanohybrid was of a controlled manner, and therefore the resulting material was suitable for use as a controlled-release formulation. HAN has synergistic properties with tamoxifen toward a HepG2 cell line, with an IC50 value of 0.35 compared with hippurate. In the antiproliferative assay, the ratio of viable cells account for cells treated by the combination tamoxifen with HAN to untreated cells was sharply reduced from 66% to 13% after 24 and 72 hours, respectively.Conclusion: The release of hippuric acid anions from HAN occurred in a controlled manner, and the resulting material is suitable for a controlled-release formulation.Keywords: hippuric acid, nanohybrids, zinc oxide, zinc-layered hydroxide, synergistic effect
format	article
author	Hussein Al Ali SH Al-Qubaisi M Hussein MZ Zainal Z Hakim MN
author_facet	Hussein Al Ali SH Al-Qubaisi M Hussein MZ Zainal Z Hakim MN
author_sort	Hussein Al Ali SH
title	Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines
title_short	Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines
title_full	Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines
title_fullStr	Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines
title_full_unstemmed	Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines
title_sort	preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on hepg2 cell lines
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/caff7d07857141e49678b4483f8db0a0
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Preparation of hippurate-zinc layered hydroxide nanohybrid and its synergistic effect with tamoxifen on HepG2 cell lines

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