Gene expression profiling of histiocytic sarcomas in a canine model: the predisposed flatcoated retriever dog.

<h4>Background</h4>The determination of altered expression of genes in specific tumor types and their effect upon cellular processes may create insight in tumorigenesis and help to design better treatments. The Flatcoated retriever is a dog breed with an exceptionally high incidence of h...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kim M Boerkamp, Marieke van der Kooij, Frank G van Steenbeek, Monique E van Wolferen, Marian J A Groot Koerkamp, Dik van Leenen, Guy C M Grinwis, Louis C Penning, Erik A C Wiemer, Gerard R Rutteman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb03fb412bee43de96f6241999709856
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cb03fb412bee43de96f6241999709856
record_format	dspace
spelling	oai:doaj.org-article:cb03fb412bee43de96f62419997098562021-11-18T09:01:37ZGene expression profiling of histiocytic sarcomas in a canine model: the predisposed flatcoated retriever dog.1932-620310.1371/journal.pone.0071094https://doaj.org/article/cb03fb412bee43de96f62419997098562013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23936488/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The determination of altered expression of genes in specific tumor types and their effect upon cellular processes may create insight in tumorigenesis and help to design better treatments. The Flatcoated retriever is a dog breed with an exceptionally high incidence of histiocytic sarcomas. The breed develops two distinct entities of histiocytic neoplasia, a soft tissue form and a visceral form. Gene expression studies of these tumors have value for comparable human diseases such as histiocytic/dendritic cell sarcoma for which knowledge is difficult to accrue due to their rare occurrence. In addition, such studies may help in the search for genetic aberrations underlying the genetic predisposition in this dog breed.<h4>Methods</h4>Microarray analysis and pathway analyses were performed on fresh-frozen tissues obtained from Flatcoated retrievers with localized, soft tissue histiocytic sarcomas (STHS) and disseminated, visceral histiocytic sarcomas (VHS) and on normal canine spleens from various breeds. Expression differences of nine genes were validated with quantitative real-time PCR (qPCR) analyses.<h4>Results</h4>QPCR analyses identified the significantly altered expression of nine genes; PPBP, SpiC, VCAM1, ENPEP, ITGAD (down-regulated), and GTSF1, Col3a1, CD90 and LUM (up-regulated) in the comparison of both the soft tissue and the visceral form with healthy spleen. DAVID pathway analyses revealed 24 pathways that were significantly involved in the development of HS in general, most of which were involved in the DNA repair and replication process.<h4>Conclusions</h4>This study identified altered expression of nine genes not yet implicated in histiocytic sarcoma manifestations in the dog nor in comparable human histiocytic/dendritic sarcomas. Exploration of the downside effect of canine inbreeding strategies for the study of similar sarcomas in humans might also lead to the identification of genes related to these rare malignancies in the human.Kim M BoerkampMarieke van der KooijFrank G van SteenbeekMonique E van WolferenMarian J A Groot KoerkampDik van LeenenGuy C M GrinwisLouis C PenningErik A C WiemerGerard R RuttemanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 8, p e71094 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kim M Boerkamp Marieke van der Kooij Frank G van Steenbeek Monique E van Wolferen Marian J A Groot Koerkamp Dik van Leenen Guy C M Grinwis Louis C Penning Erik A C Wiemer Gerard R Rutteman Gene expression profiling of histiocytic sarcomas in a canine model: the predisposed flatcoated retriever dog.
description	<h4>Background</h4>The determination of altered expression of genes in specific tumor types and their effect upon cellular processes may create insight in tumorigenesis and help to design better treatments. The Flatcoated retriever is a dog breed with an exceptionally high incidence of histiocytic sarcomas. The breed develops two distinct entities of histiocytic neoplasia, a soft tissue form and a visceral form. Gene expression studies of these tumors have value for comparable human diseases such as histiocytic/dendritic cell sarcoma for which knowledge is difficult to accrue due to their rare occurrence. In addition, such studies may help in the search for genetic aberrations underlying the genetic predisposition in this dog breed.<h4>Methods</h4>Microarray analysis and pathway analyses were performed on fresh-frozen tissues obtained from Flatcoated retrievers with localized, soft tissue histiocytic sarcomas (STHS) and disseminated, visceral histiocytic sarcomas (VHS) and on normal canine spleens from various breeds. Expression differences of nine genes were validated with quantitative real-time PCR (qPCR) analyses.<h4>Results</h4>QPCR analyses identified the significantly altered expression of nine genes; PPBP, SpiC, VCAM1, ENPEP, ITGAD (down-regulated), and GTSF1, Col3a1, CD90 and LUM (up-regulated) in the comparison of both the soft tissue and the visceral form with healthy spleen. DAVID pathway analyses revealed 24 pathways that were significantly involved in the development of HS in general, most of which were involved in the DNA repair and replication process.<h4>Conclusions</h4>This study identified altered expression of nine genes not yet implicated in histiocytic sarcoma manifestations in the dog nor in comparable human histiocytic/dendritic sarcomas. Exploration of the downside effect of canine inbreeding strategies for the study of similar sarcomas in humans might also lead to the identification of genes related to these rare malignancies in the human.
format	article
author	Kim M Boerkamp Marieke van der Kooij Frank G van Steenbeek Monique E van Wolferen Marian J A Groot Koerkamp Dik van Leenen Guy C M Grinwis Louis C Penning Erik A C Wiemer Gerard R Rutteman
author_facet	Kim M Boerkamp Marieke van der Kooij Frank G van Steenbeek Monique E van Wolferen Marian J A Groot Koerkamp Dik van Leenen Guy C M Grinwis Louis C Penning Erik A C Wiemer Gerard R Rutteman
author_sort	Kim M Boerkamp
title	Gene expression profiling of histiocytic sarcomas in a canine model: the predisposed flatcoated retriever dog.
title_short	Gene expression profiling of histiocytic sarcomas in a canine model: the predisposed flatcoated retriever dog.
title_full	Gene expression profiling of histiocytic sarcomas in a canine model: the predisposed flatcoated retriever dog.
title_fullStr	Gene expression profiling of histiocytic sarcomas in a canine model: the predisposed flatcoated retriever dog.
title_full_unstemmed	Gene expression profiling of histiocytic sarcomas in a canine model: the predisposed flatcoated retriever dog.
title_sort	gene expression profiling of histiocytic sarcomas in a canine model: the predisposed flatcoated retriever dog.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/cb03fb412bee43de96f6241999709856
work_keys_str_mv	AT kimmboerkamp geneexpressionprofilingofhistiocyticsarcomasinacaninemodelthepredisposedflatcoatedretrieverdog AT mariekevanderkooij geneexpressionprofilingofhistiocyticsarcomasinacaninemodelthepredisposedflatcoatedretrieverdog AT frankgvansteenbeek geneexpressionprofilingofhistiocyticsarcomasinacaninemodelthepredisposedflatcoatedretrieverdog AT moniqueevanwolferen geneexpressionprofilingofhistiocyticsarcomasinacaninemodelthepredisposedflatcoatedretrieverdog AT marianjagrootkoerkamp geneexpressionprofilingofhistiocyticsarcomasinacaninemodelthepredisposedflatcoatedretrieverdog AT dikvanleenen geneexpressionprofilingofhistiocyticsarcomasinacaninemodelthepredisposedflatcoatedretrieverdog AT guycmgrinwis geneexpressionprofilingofhistiocyticsarcomasinacaninemodelthepredisposedflatcoatedretrieverdog AT louiscpenning geneexpressionprofilingofhistiocyticsarcomasinacaninemodelthepredisposedflatcoatedretrieverdog AT erikacwiemer geneexpressionprofilingofhistiocyticsarcomasinacaninemodelthepredisposedflatcoatedretrieverdog AT gerardrrutteman geneexpressionprofilingofhistiocyticsarcomasinacaninemodelthepredisposedflatcoatedretrieverdog
_version_	1718421049487392768

Gene expression profiling of histiocytic sarcomas in a canine model: the predisposed flatcoated retriever dog.

Ejemplares similares