Cloning and characterization of maize miRNAs involved in responses to nitrogen deficiency.

Although recent studies indicated that miRNAs regulate plant adaptive responses to nutrient deprivation, the functional significance of miRNAs in adaptive responses to nitrogen (N) limitation remains to be explored. To elucidate the molecular biology underlying N sensing/signaling in maize, we const...

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Autores principales:	Meng Zhao, Huanhuan Tai, Suzhen Sun, Fusuo Zhang, Yunbi Xu, Wen-Xue Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb0558b98c8f476c9daf3c5dd1e18152
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spelling	oai:doaj.org-article:cb0558b98c8f476c9daf3c5dd1e181522021-11-18T07:31:05ZCloning and characterization of maize miRNAs involved in responses to nitrogen deficiency.1932-620310.1371/journal.pone.0029669https://doaj.org/article/cb0558b98c8f476c9daf3c5dd1e181522012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22235323/?tool=EBIhttps://doaj.org/toc/1932-6203Although recent studies indicated that miRNAs regulate plant adaptive responses to nutrient deprivation, the functional significance of miRNAs in adaptive responses to nitrogen (N) limitation remains to be explored. To elucidate the molecular biology underlying N sensing/signaling in maize, we constructed four small RNA libraries and one degradome from maize seedlings exposed to N deficiency. We discovered a total of 99 absolutely new loci belonging to 47 miRNA families by small RNA deep sequencing and degradome sequencing, as well as 9 new loci were the paralogs of previously reported miR169, miR171, and miR398, significantly expanding the reported 150 high confidence genes within 26 miRNA families in maize. Bioinformatic and subsequent small RNA northern blot analysis identified eight miRNA families (five conserved and three newly identified) differentially expressed under the N-deficient condition. Predicted and degradome-validated targets of the newly identified miRNAs suggest their involvement in a broad range of cellular responses and metabolic processes. Because maize is not only an important crop but is also a genetic model for basic biological research, our research contributes to the understanding of the regulatory roles of miRNAs in plant adaption to N-deficiency stress.Meng ZhaoHuanhuan TaiSuzhen SunFusuo ZhangYunbi XuWen-Xue LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e29669 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meng Zhao Huanhuan Tai Suzhen Sun Fusuo Zhang Yunbi Xu Wen-Xue Li Cloning and characterization of maize miRNAs involved in responses to nitrogen deficiency.
description	Although recent studies indicated that miRNAs regulate plant adaptive responses to nutrient deprivation, the functional significance of miRNAs in adaptive responses to nitrogen (N) limitation remains to be explored. To elucidate the molecular biology underlying N sensing/signaling in maize, we constructed four small RNA libraries and one degradome from maize seedlings exposed to N deficiency. We discovered a total of 99 absolutely new loci belonging to 47 miRNA families by small RNA deep sequencing and degradome sequencing, as well as 9 new loci were the paralogs of previously reported miR169, miR171, and miR398, significantly expanding the reported 150 high confidence genes within 26 miRNA families in maize. Bioinformatic and subsequent small RNA northern blot analysis identified eight miRNA families (five conserved and three newly identified) differentially expressed under the N-deficient condition. Predicted and degradome-validated targets of the newly identified miRNAs suggest their involvement in a broad range of cellular responses and metabolic processes. Because maize is not only an important crop but is also a genetic model for basic biological research, our research contributes to the understanding of the regulatory roles of miRNAs in plant adaption to N-deficiency stress.
format	article
author	Meng Zhao Huanhuan Tai Suzhen Sun Fusuo Zhang Yunbi Xu Wen-Xue Li
author_facet	Meng Zhao Huanhuan Tai Suzhen Sun Fusuo Zhang Yunbi Xu Wen-Xue Li
author_sort	Meng Zhao
title	Cloning and characterization of maize miRNAs involved in responses to nitrogen deficiency.
title_short	Cloning and characterization of maize miRNAs involved in responses to nitrogen deficiency.
title_full	Cloning and characterization of maize miRNAs involved in responses to nitrogen deficiency.
title_fullStr	Cloning and characterization of maize miRNAs involved in responses to nitrogen deficiency.
title_full_unstemmed	Cloning and characterization of maize miRNAs involved in responses to nitrogen deficiency.
title_sort	cloning and characterization of maize mirnas involved in responses to nitrogen deficiency.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/cb0558b98c8f476c9daf3c5dd1e18152
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Cloning and characterization of maize miRNAs involved in responses to nitrogen deficiency.

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