Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements

Here, the authors show that reticulocytes derived from immortalized erythroblasts support invasion and development of Plasmodium falciparum and use CRISPR-mediated gene knockout and complementation of an invasion receptor to demonstrate utility of this model system for research in malaria invasion.

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Autores principales:	Timothy J. Satchwell, Katherine E. Wright, Katy L. Haydn-Smith, Fernando Sánchez-Román Terán, Pedro L. Moura, Joseph Hawksworth, Jan Frayne, Ashley M. Toye, Jake Baum
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/cb05acb78a2c45d997fced9f3abed603
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spelling	oai:doaj.org-article:cb05acb78a2c45d997fced9f3abed6032021-12-02T15:35:55ZGenetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements10.1038/s41467-019-11790-w2041-1723https://doaj.org/article/cb05acb78a2c45d997fced9f3abed6032019-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11790-whttps://doaj.org/toc/2041-1723Here, the authors show that reticulocytes derived from immortalized erythroblasts support invasion and development of Plasmodium falciparum and use CRISPR-mediated gene knockout and complementation of an invasion receptor to demonstrate utility of this model system for research in malaria invasion.Timothy J. SatchwellKatherine E. WrightKaty L. Haydn-SmithFernando Sánchez-Román TeránPedro L. MouraJoseph HawksworthJan FrayneAshley M. ToyeJake BaumNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Timothy J. Satchwell Katherine E. Wright Katy L. Haydn-Smith Fernando Sánchez-Román Terán Pedro L. Moura Joseph Hawksworth Jan Frayne Ashley M. Toye Jake Baum Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements
description	Here, the authors show that reticulocytes derived from immortalized erythroblasts support invasion and development of Plasmodium falciparum and use CRISPR-mediated gene knockout and complementation of an invasion receptor to demonstrate utility of this model system for research in malaria invasion.
format	article
author	Timothy J. Satchwell Katherine E. Wright Katy L. Haydn-Smith Fernando Sánchez-Román Terán Pedro L. Moura Joseph Hawksworth Jan Frayne Ashley M. Toye Jake Baum
author_facet	Timothy J. Satchwell Katherine E. Wright Katy L. Haydn-Smith Fernando Sánchez-Román Terán Pedro L. Moura Joseph Hawksworth Jan Frayne Ashley M. Toye Jake Baum
author_sort	Timothy J. Satchwell
title	Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements
title_short	Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements
title_full	Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements
title_fullStr	Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements
title_full_unstemmed	Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements
title_sort	genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/cb05acb78a2c45d997fced9f3abed603
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Genetic manipulation of cell line derived reticulocytes enables dissection of host malaria invasion requirements

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