Construction of ferritin hydrogels utilizing subunit–subunit interactions

Various proteins form nanostructures exhibiting unique functions, making them attractive as next-generation materials. Ferritin is a hollow spherical protein that incorporates iron ions. Here, we found that hydrogels are simply formed from concentrated apoferritin solutions by acid denaturation and...

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Autores principales:	Masaru Yamanaka, Tsuyoshi Mashima, Michio Ogihara, Mei Okamoto, Takayuki Uchihashi, Shun Hirota
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb0fffb40c2a40249b44c9b51e9b3fd4
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spelling	oai:doaj.org-article:cb0fffb40c2a40249b44c9b51e9b3fd42021-11-11T07:14:46ZConstruction of ferritin hydrogels utilizing subunit–subunit interactions1932-6203https://doaj.org/article/cb0fffb40c2a40249b44c9b51e9b3fd42021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565734/?tool=EBIhttps://doaj.org/toc/1932-6203Various proteins form nanostructures exhibiting unique functions, making them attractive as next-generation materials. Ferritin is a hollow spherical protein that incorporates iron ions. Here, we found that hydrogels are simply formed from concentrated apoferritin solutions by acid denaturation and subsequent neutralization. The water content of the hydrogel was approximately 80%. The apoferritin hydrogel did not decompose in the presence of 1 M HCl, 2-mercaptoethanol, or methanol but was dissolved in the presence of 1 M NaOH, by heating at 80°C, or by treatment with trypsin or 6 M guanidine hydrochloride. The Young’s modulus of the hydrogel was 20.4 ± 12.1 kPa according to local indentation experimentes using atomic force microscopy, indicating that the hydrogel was relatively stiff. Transition electron microscopy measurements revealed that a fibrous network was constructed in the hydrogel. The color of the hydrogel became yellow-brown upon incubation in the presence of Fe3+ ions, indicating that the hydrogel adsorbed the Fe3+ ions. The yellow-brown color of the Fe3+-adsorbed hydrogel did not change upon incubation in pure water, whereas it became pale by incubating it in the presence of 100 mM ethylenediaminetetraacetic acid (EDTA). The apoferritin hydrogel also adsorbed Co2+ and Cu2+ ions and released them in the presence of EDTA, while it adsorbed less Ni2+ ions; more Fe3+ ions adsorbed to the apoferritin hydrogel than other metal ions, indicating that the hydrogel keeps the iron storage characteristic of ferritin. These results demonstrate a new property of ferritin: the ability to form a hydrogel that can adsorb/desorb metal ions, which may be useful in designing future biomaterials.Masaru YamanakaTsuyoshi MashimaMichio OgiharaMei OkamotoTakayuki UchihashiShun HirotaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masaru Yamanaka Tsuyoshi Mashima Michio Ogihara Mei Okamoto Takayuki Uchihashi Shun Hirota Construction of ferritin hydrogels utilizing subunit–subunit interactions
description	Various proteins form nanostructures exhibiting unique functions, making them attractive as next-generation materials. Ferritin is a hollow spherical protein that incorporates iron ions. Here, we found that hydrogels are simply formed from concentrated apoferritin solutions by acid denaturation and subsequent neutralization. The water content of the hydrogel was approximately 80%. The apoferritin hydrogel did not decompose in the presence of 1 M HCl, 2-mercaptoethanol, or methanol but was dissolved in the presence of 1 M NaOH, by heating at 80°C, or by treatment with trypsin or 6 M guanidine hydrochloride. The Young’s modulus of the hydrogel was 20.4 ± 12.1 kPa according to local indentation experimentes using atomic force microscopy, indicating that the hydrogel was relatively stiff. Transition electron microscopy measurements revealed that a fibrous network was constructed in the hydrogel. The color of the hydrogel became yellow-brown upon incubation in the presence of Fe3+ ions, indicating that the hydrogel adsorbed the Fe3+ ions. The yellow-brown color of the Fe3+-adsorbed hydrogel did not change upon incubation in pure water, whereas it became pale by incubating it in the presence of 100 mM ethylenediaminetetraacetic acid (EDTA). The apoferritin hydrogel also adsorbed Co2+ and Cu2+ ions and released them in the presence of EDTA, while it adsorbed less Ni2+ ions; more Fe3+ ions adsorbed to the apoferritin hydrogel than other metal ions, indicating that the hydrogel keeps the iron storage characteristic of ferritin. These results demonstrate a new property of ferritin: the ability to form a hydrogel that can adsorb/desorb metal ions, which may be useful in designing future biomaterials.
format	article
author	Masaru Yamanaka Tsuyoshi Mashima Michio Ogihara Mei Okamoto Takayuki Uchihashi Shun Hirota
author_facet	Masaru Yamanaka Tsuyoshi Mashima Michio Ogihara Mei Okamoto Takayuki Uchihashi Shun Hirota
author_sort	Masaru Yamanaka
title	Construction of ferritin hydrogels utilizing subunit–subunit interactions
title_short	Construction of ferritin hydrogels utilizing subunit–subunit interactions
title_full	Construction of ferritin hydrogels utilizing subunit–subunit interactions
title_fullStr	Construction of ferritin hydrogels utilizing subunit–subunit interactions
title_full_unstemmed	Construction of ferritin hydrogels utilizing subunit–subunit interactions
title_sort	construction of ferritin hydrogels utilizing subunit–subunit interactions
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/cb0fffb40c2a40249b44c9b51e9b3fd4
work_keys_str_mv	AT masaruyamanaka constructionofferritinhydrogelsutilizingsubunitsubunitinteractions AT tsuyoshimashima constructionofferritinhydrogelsutilizingsubunitsubunitinteractions AT michioogihara constructionofferritinhydrogelsutilizingsubunitsubunitinteractions AT meiokamoto constructionofferritinhydrogelsutilizingsubunitsubunitinteractions AT takayukiuchihashi constructionofferritinhydrogelsutilizingsubunitsubunitinteractions AT shunhirota constructionofferritinhydrogelsutilizingsubunitsubunitinteractions
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Construction of ferritin hydrogels utilizing subunit–subunit interactions

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