Mapacalcine protects mouse neurons against hypoxia by blocking cell calcium overload.

Stroke is one of a major cause of death and adult disability. Despite intense researches, treatment for stroke remains reduced to fibrinolysis, a technique useful for less than 10% of patients. Finding molecules able to treat or at least to decrease the deleterious consequences of stroke is an urgen...

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Autores principales:	Hamid Moha Ou Maati, Catherine Widmann, Djamila Sedjelmaci, Bernard Gallois, Catherine Heurteaux, Marc Borsotto, Michel Hugues
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb11dc94c9884aa9bd9f780fee077e0f
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spelling	oai:doaj.org-article:cb11dc94c9884aa9bd9f780fee077e0f2021-11-18T07:39:11ZMapacalcine protects mouse neurons against hypoxia by blocking cell calcium overload.1932-620310.1371/journal.pone.0066194https://doaj.org/article/cb11dc94c9884aa9bd9f780fee077e0f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23843951/?tool=EBIhttps://doaj.org/toc/1932-6203Stroke is one of a major cause of death and adult disability. Despite intense researches, treatment for stroke remains reduced to fibrinolysis, a technique useful for less than 10% of patients. Finding molecules able to treat or at least to decrease the deleterious consequences of stroke is an urgent need. Here, we showed that mapacalcine, a homodimeric peptide purified from the marine sponge Cliona vastifica, is able to protect mouse cortical neurons against hypoxia. We have also identified a subtype of L-type calcium channel as a target for mapacalcine and we showed that the channel has to be open for mapacalcine binding. The two main L-type subunits at the brain level are CaV1.3 and CaV1.2 subunits but mapacalcine was unable to block these calcium channels.Mapacalcine did not interfere with N-, P/Q- and R-type calcium channels. The protective effect was studied by measuring internal calcium level variation triggered by Oxygen Glucose Deprivation protocol, which mimics stroke, or glutamate stimulation. We showed that NMDA/AMPA receptors are not involved in the mapacalcine protection. The protective effect was confirmed by measuring the cell survival rate after Oxygen Glucose Deprivation condition. Our data indicate that mapacalcine is a promising molecule for stroke treatment.Hamid Moha Ou MaatiCatherine WidmannDjamila SedjelmaciBernard GalloisCatherine HeurteauxMarc BorsottoMichel HuguesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e66194 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hamid Moha Ou Maati Catherine Widmann Djamila Sedjelmaci Bernard Gallois Catherine Heurteaux Marc Borsotto Michel Hugues Mapacalcine protects mouse neurons against hypoxia by blocking cell calcium overload.
description	Stroke is one of a major cause of death and adult disability. Despite intense researches, treatment for stroke remains reduced to fibrinolysis, a technique useful for less than 10% of patients. Finding molecules able to treat or at least to decrease the deleterious consequences of stroke is an urgent need. Here, we showed that mapacalcine, a homodimeric peptide purified from the marine sponge Cliona vastifica, is able to protect mouse cortical neurons against hypoxia. We have also identified a subtype of L-type calcium channel as a target for mapacalcine and we showed that the channel has to be open for mapacalcine binding. The two main L-type subunits at the brain level are CaV1.3 and CaV1.2 subunits but mapacalcine was unable to block these calcium channels.Mapacalcine did not interfere with N-, P/Q- and R-type calcium channels. The protective effect was studied by measuring internal calcium level variation triggered by Oxygen Glucose Deprivation protocol, which mimics stroke, or glutamate stimulation. We showed that NMDA/AMPA receptors are not involved in the mapacalcine protection. The protective effect was confirmed by measuring the cell survival rate after Oxygen Glucose Deprivation condition. Our data indicate that mapacalcine is a promising molecule for stroke treatment.
format	article
author	Hamid Moha Ou Maati Catherine Widmann Djamila Sedjelmaci Bernard Gallois Catherine Heurteaux Marc Borsotto Michel Hugues
author_facet	Hamid Moha Ou Maati Catherine Widmann Djamila Sedjelmaci Bernard Gallois Catherine Heurteaux Marc Borsotto Michel Hugues
author_sort	Hamid Moha Ou Maati
title	Mapacalcine protects mouse neurons against hypoxia by blocking cell calcium overload.
title_short	Mapacalcine protects mouse neurons against hypoxia by blocking cell calcium overload.
title_full	Mapacalcine protects mouse neurons against hypoxia by blocking cell calcium overload.
title_fullStr	Mapacalcine protects mouse neurons against hypoxia by blocking cell calcium overload.
title_full_unstemmed	Mapacalcine protects mouse neurons against hypoxia by blocking cell calcium overload.
title_sort	mapacalcine protects mouse neurons against hypoxia by blocking cell calcium overload.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/cb11dc94c9884aa9bd9f780fee077e0f
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Mapacalcine protects mouse neurons against hypoxia by blocking cell calcium overload.

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