The algicidal mechanism of prodigiosin from Hahella sp. KA22 against Microcystis aeruginosa

Abstract In recent years, Microcystis aeruginosa blooms have occurred throughout the world, causing huge economic losses and destroying aquatic ecosystems. It is necessary to develop effective and ecofriendly methods to control M. aeruginosa blooms. Here, we report a high algicidal activity of prodi...

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Autores principales:	Ke Yang, Qiuliang Chen, Danyang Zhang, Huajun Zhang, Xueqian Lei, Zhangran Chen, Yi Li, Yaling Hong, Xiaohong Ma, Wei Zheng, Yun Tian, Tianling Zheng, Hong Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb2733fe6b5044cc997931ff195ea355
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spelling	oai:doaj.org-article:cb2733fe6b5044cc997931ff195ea3552021-12-02T16:06:17ZThe algicidal mechanism of prodigiosin from Hahella sp. KA22 against Microcystis aeruginosa10.1038/s41598-017-08132-52045-2322https://doaj.org/article/cb2733fe6b5044cc997931ff195ea3552017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08132-5https://doaj.org/toc/2045-2322Abstract In recent years, Microcystis aeruginosa blooms have occurred throughout the world, causing huge economic losses and destroying aquatic ecosystems. It is necessary to develop effective and ecofriendly methods to control M. aeruginosa blooms. Here, we report a high algicidal activity of prodigiosin (PG) against M. aeruginosa as well as the algicidal mechanism. PG showed high algicidal activity against M. aeruginosa, with a 50% lethal dose (LD50) of 5.87 μg/mL in 72 h. A combination of methods, including propidium iodide and Annexin V-fluorescein staining assays and light and electron microscopy indicated the existence of two modes of cell death with features similar to those in eukaryotic programmed cell death: necrotic-like and apoptotic-like. Biochemical and physiological analyses showed that PG generates reactive oxygen species (ROS), which induce lipid peroxidation, damage the membrane system and destroy the function of the photosystem. A proteomics analysis revealed that many proteins were differentially expressed in response to PG stress and that most of these proteins were involved in important metabolic processes, which may trigger necrotic-like or apoptotic-like cell death. The present study sheds light on the multiple toxicity mechanisms of PG on M. aeruginosa and its potential for controlling the occurrence of M. aeruginosa blooms in lakes.Ke YangQiuliang ChenDanyang ZhangHuajun ZhangXueqian LeiZhangran ChenYi LiYaling HongXiaohong MaWei ZhengYun TianTianling ZhengHong XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ke Yang Qiuliang Chen Danyang Zhang Huajun Zhang Xueqian Lei Zhangran Chen Yi Li Yaling Hong Xiaohong Ma Wei Zheng Yun Tian Tianling Zheng Hong Xu The algicidal mechanism of prodigiosin from Hahella sp. KA22 against Microcystis aeruginosa
description	Abstract In recent years, Microcystis aeruginosa blooms have occurred throughout the world, causing huge economic losses and destroying aquatic ecosystems. It is necessary to develop effective and ecofriendly methods to control M. aeruginosa blooms. Here, we report a high algicidal activity of prodigiosin (PG) against M. aeruginosa as well as the algicidal mechanism. PG showed high algicidal activity against M. aeruginosa, with a 50% lethal dose (LD50) of 5.87 μg/mL in 72 h. A combination of methods, including propidium iodide and Annexin V-fluorescein staining assays and light and electron microscopy indicated the existence of two modes of cell death with features similar to those in eukaryotic programmed cell death: necrotic-like and apoptotic-like. Biochemical and physiological analyses showed that PG generates reactive oxygen species (ROS), which induce lipid peroxidation, damage the membrane system and destroy the function of the photosystem. A proteomics analysis revealed that many proteins were differentially expressed in response to PG stress and that most of these proteins were involved in important metabolic processes, which may trigger necrotic-like or apoptotic-like cell death. The present study sheds light on the multiple toxicity mechanisms of PG on M. aeruginosa and its potential for controlling the occurrence of M. aeruginosa blooms in lakes.
format	article
author	Ke Yang Qiuliang Chen Danyang Zhang Huajun Zhang Xueqian Lei Zhangran Chen Yi Li Yaling Hong Xiaohong Ma Wei Zheng Yun Tian Tianling Zheng Hong Xu
author_facet	Ke Yang Qiuliang Chen Danyang Zhang Huajun Zhang Xueqian Lei Zhangran Chen Yi Li Yaling Hong Xiaohong Ma Wei Zheng Yun Tian Tianling Zheng Hong Xu
author_sort	Ke Yang
title	The algicidal mechanism of prodigiosin from Hahella sp. KA22 against Microcystis aeruginosa
title_short	The algicidal mechanism of prodigiosin from Hahella sp. KA22 against Microcystis aeruginosa
title_full	The algicidal mechanism of prodigiosin from Hahella sp. KA22 against Microcystis aeruginosa
title_fullStr	The algicidal mechanism of prodigiosin from Hahella sp. KA22 against Microcystis aeruginosa
title_full_unstemmed	The algicidal mechanism of prodigiosin from Hahella sp. KA22 against Microcystis aeruginosa
title_sort	algicidal mechanism of prodigiosin from hahella sp. ka22 against microcystis aeruginosa
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cb2733fe6b5044cc997931ff195ea355
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The algicidal mechanism of prodigiosin from Hahella sp. KA22 against Microcystis aeruginosa

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