Dynamic gene expression response to altered gravity in human T cells

Abstract We investigated the dynamics of immediate and initial gene expression response to different gravitational environments in human Jurkat T lymphocytic cells and compared expression profiles to identify potential gravity-regulated genes and adaptation processes. We used the Affymetrix GeneChip...

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Autores principales:	Cora S. Thiel, Swantje Hauschild, Andreas Huge, Svantje Tauber, Beatrice A. Lauber, Jennifer Polzer, Katrin Paulsen, Hartwin Lier, Frank Engelmann, Burkhard Schmitz, Andreas Schütte, Liliana E. Layer, Oliver Ullrich
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb286fb18e6c487984773382255761ca
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spelling	oai:doaj.org-article:cb286fb18e6c487984773382255761ca2021-12-02T16:06:21ZDynamic gene expression response to altered gravity in human T cells10.1038/s41598-017-05580-x2045-2322https://doaj.org/article/cb286fb18e6c487984773382255761ca2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05580-xhttps://doaj.org/toc/2045-2322Abstract We investigated the dynamics of immediate and initial gene expression response to different gravitational environments in human Jurkat T lymphocytic cells and compared expression profiles to identify potential gravity-regulated genes and adaptation processes. We used the Affymetrix GeneChip® Human Transcriptome Array 2.0 containing 44,699 protein coding genes and 22,829 non-protein coding genes and performed the experiments during a parabolic flight and a suborbital ballistic rocket mission to cross-validate gravity-regulated gene expression through independent research platforms and different sets of control experiments to exclude other factors than alteration of gravity. We found that gene expression in human T cells rapidly responded to altered gravity in the time frame of 20 s and 5 min. The initial response to microgravity involved mostly regulatory RNAs. We identified three gravity-regulated genes which could be cross-validated in both completely independent experiment missions: ATP6V1A/D, a vacuolar H + -ATPase (V-ATPase) responsible for acidification during bone resorption, IGHD3-3/IGHD3-10, diversity genes of the immunoglobulin heavy-chain locus participating in V(D)J recombination, and LINC00837, a long intergenic non-protein coding RNA. Due to the extensive and rapid alteration of gene expression associated with regulatory RNAs, we conclude that human cells are equipped with a robust and efficient adaptation potential when challenged with altered gravitational environments.Cora S. ThielSwantje HauschildAndreas HugeSvantje TauberBeatrice A. LauberJennifer PolzerKatrin PaulsenHartwin LierFrank EngelmannBurkhard SchmitzAndreas SchütteLiliana E. LayerOliver UllrichNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-22 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cora S. Thiel Swantje Hauschild Andreas Huge Svantje Tauber Beatrice A. Lauber Jennifer Polzer Katrin Paulsen Hartwin Lier Frank Engelmann Burkhard Schmitz Andreas Schütte Liliana E. Layer Oliver Ullrich Dynamic gene expression response to altered gravity in human T cells
description	Abstract We investigated the dynamics of immediate and initial gene expression response to different gravitational environments in human Jurkat T lymphocytic cells and compared expression profiles to identify potential gravity-regulated genes and adaptation processes. We used the Affymetrix GeneChip® Human Transcriptome Array 2.0 containing 44,699 protein coding genes and 22,829 non-protein coding genes and performed the experiments during a parabolic flight and a suborbital ballistic rocket mission to cross-validate gravity-regulated gene expression through independent research platforms and different sets of control experiments to exclude other factors than alteration of gravity. We found that gene expression in human T cells rapidly responded to altered gravity in the time frame of 20 s and 5 min. The initial response to microgravity involved mostly regulatory RNAs. We identified three gravity-regulated genes which could be cross-validated in both completely independent experiment missions: ATP6V1A/D, a vacuolar H + -ATPase (V-ATPase) responsible for acidification during bone resorption, IGHD3-3/IGHD3-10, diversity genes of the immunoglobulin heavy-chain locus participating in V(D)J recombination, and LINC00837, a long intergenic non-protein coding RNA. Due to the extensive and rapid alteration of gene expression associated with regulatory RNAs, we conclude that human cells are equipped with a robust and efficient adaptation potential when challenged with altered gravitational environments.
format	article
author	Cora S. Thiel Swantje Hauschild Andreas Huge Svantje Tauber Beatrice A. Lauber Jennifer Polzer Katrin Paulsen Hartwin Lier Frank Engelmann Burkhard Schmitz Andreas Schütte Liliana E. Layer Oliver Ullrich
author_facet	Cora S. Thiel Swantje Hauschild Andreas Huge Svantje Tauber Beatrice A. Lauber Jennifer Polzer Katrin Paulsen Hartwin Lier Frank Engelmann Burkhard Schmitz Andreas Schütte Liliana E. Layer Oliver Ullrich
author_sort	Cora S. Thiel
title	Dynamic gene expression response to altered gravity in human T cells
title_short	Dynamic gene expression response to altered gravity in human T cells
title_full	Dynamic gene expression response to altered gravity in human T cells
title_fullStr	Dynamic gene expression response to altered gravity in human T cells
title_full_unstemmed	Dynamic gene expression response to altered gravity in human T cells
title_sort	dynamic gene expression response to altered gravity in human t cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cb286fb18e6c487984773382255761ca
work_keys_str_mv	AT corasthiel dynamicgeneexpressionresponsetoalteredgravityinhumantcells AT swantjehauschild dynamicgeneexpressionresponsetoalteredgravityinhumantcells AT andreashuge dynamicgeneexpressionresponsetoalteredgravityinhumantcells AT svantjetauber dynamicgeneexpressionresponsetoalteredgravityinhumantcells AT beatricealauber dynamicgeneexpressionresponsetoalteredgravityinhumantcells AT jenniferpolzer dynamicgeneexpressionresponsetoalteredgravityinhumantcells AT katrinpaulsen dynamicgeneexpressionresponsetoalteredgravityinhumantcells AT hartwinlier dynamicgeneexpressionresponsetoalteredgravityinhumantcells AT frankengelmann dynamicgeneexpressionresponsetoalteredgravityinhumantcells AT burkhardschmitz dynamicgeneexpressionresponsetoalteredgravityinhumantcells AT andreasschutte dynamicgeneexpressionresponsetoalteredgravityinhumantcells AT lilianaelayer dynamicgeneexpressionresponsetoalteredgravityinhumantcells AT oliverullrich dynamicgeneexpressionresponsetoalteredgravityinhumantcells
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Dynamic gene expression response to altered gravity in human T cells

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