Screening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense

Abstract. Yurnaliza. Rambe DI, Sarimunggu L, Purba M, Nurwahyuni I, Lenny S, Lutfia A, Hartanto A. 2020. Screening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense. Biodiversitas 21: 3431-3437. Burkholderia spp. are ubiquitous diazotrophic bacte...

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Autores principales:	YURNALIZA YURNALIZA, DITA ISNAINI RAMBE, LISTRA SARIMUNGGU, MEYLISA PURBA, ISNAINI NURWAHYUNI, SOVIA LENNY, ANISA LUTFIA, ADRIAN HARTANTO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	16s rdna, basal stem rot, burkholderia contaminans, elaeis guineensis, ganoderma boninense Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cb310b58f80c46f7a4b6b1676221834f
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spelling	oai:doaj.org-article:cb310b58f80c46f7a4b6b1676221834f2021-11-22T12:17:57ZScreening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense1412-033X2085-472210.13057/biodiv/d210803https://doaj.org/article/cb310b58f80c46f7a4b6b1676221834f2020-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6215https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Yurnaliza. Rambe DI, Sarimunggu L, Purba M, Nurwahyuni I, Lenny S, Lutfia A, Hartanto A. 2020. Screening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense. Biodiversitas 21: 3431-3437. Burkholderia spp. are ubiquitous diazotrophic bacteria within ?-Proteobacteria group, known for its occurrence in variety of niches from aquatic to terrestrial habitats and as endophytes. Beneficial strains of Burkholderia have been reported as plant growth-promoting rhizobacteria or as antagonistic bacteria against phytopathogenic fungi. This study evaluated the potential of multiple strains of Burkholderia spp. recovered from three ecological niches, such as rhizosphere, humus soil, and root endophytes of oil palm plantations in North Sumatra to suppress the growth of basal stem rot causative agent in oil palm (Elaeis guineensis Jacq.) by Ganoderma boninense Pat. The antagonistic isolates were identified on the basis of molecular identification using 16S rDNA sequence (27F–1462R), revealing twelve isolates (48%) as members of Burkholderia cepacia complex (Bcc), with other minor genera, such as Chitinophaga, Klebsiella, Mycobacterium, Paenibacillus, Rhizobium, Serratia, Stenotrophomonas, and Xanthomonas. The antagonistic activities as expressed in the percentage inhibition of radial growth (PIRG) against G. boninense were considerably potential with the highest percentage of 55%. In comparison, the crude extract (MeOH, EtOAc) was also tested against G. boninense colonies showing PIRG from 0 to 38%. Majority of isolates did not show any visible chitinolytic activity based on plate assay, in exception to B. contaminans RC02 while most of them were producers of glucanase. The collection of indigenous Burkholderia spp. originating from North Sumatran oil palm plantations, i.e. B. cepacia, B. contaminans, B. metallica, and B. stagnalis may then be considered as potential biocontrol agents against G. boninense based on their antagonistic activities, antifungal properties, and hydrolytic enzyme activities.YURNALIZA YURNALIZADITA ISNAINI RAMBELISTRA SARIMUNGGUMEYLISA PURBAISNAINI NURWAHYUNISOVIA LENNYANISA LUTFIAADRIAN HARTANTOMBI & UNS Soloarticle16s rdna, basal stem rot, burkholderia contaminans, elaeis guineensis, ganoderma boninenseBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rdna, basal stem rot, burkholderia contaminans, elaeis guineensis, ganoderma boninense Biology (General) QH301-705.5
spellingShingle	16s rdna, basal stem rot, burkholderia contaminans, elaeis guineensis, ganoderma boninense Biology (General) QH301-705.5 YURNALIZA YURNALIZA DITA ISNAINI RAMBE LISTRA SARIMUNGGU MEYLISA PURBA ISNAINI NURWAHYUNI SOVIA LENNY ANISA LUTFIA ADRIAN HARTANTO Screening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense
description	Abstract. Yurnaliza. Rambe DI, Sarimunggu L, Purba M, Nurwahyuni I, Lenny S, Lutfia A, Hartanto A. 2020. Screening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense. Biodiversitas 21: 3431-3437. Burkholderia spp. are ubiquitous diazotrophic bacteria within ?-Proteobacteria group, known for its occurrence in variety of niches from aquatic to terrestrial habitats and as endophytes. Beneficial strains of Burkholderia have been reported as plant growth-promoting rhizobacteria or as antagonistic bacteria against phytopathogenic fungi. This study evaluated the potential of multiple strains of Burkholderia spp. recovered from three ecological niches, such as rhizosphere, humus soil, and root endophytes of oil palm plantations in North Sumatra to suppress the growth of basal stem rot causative agent in oil palm (Elaeis guineensis Jacq.) by Ganoderma boninense Pat. The antagonistic isolates were identified on the basis of molecular identification using 16S rDNA sequence (27F–1462R), revealing twelve isolates (48%) as members of Burkholderia cepacia complex (Bcc), with other minor genera, such as Chitinophaga, Klebsiella, Mycobacterium, Paenibacillus, Rhizobium, Serratia, Stenotrophomonas, and Xanthomonas. The antagonistic activities as expressed in the percentage inhibition of radial growth (PIRG) against G. boninense were considerably potential with the highest percentage of 55%. In comparison, the crude extract (MeOH, EtOAc) was also tested against G. boninense colonies showing PIRG from 0 to 38%. Majority of isolates did not show any visible chitinolytic activity based on plate assay, in exception to B. contaminans RC02 while most of them were producers of glucanase. The collection of indigenous Burkholderia spp. originating from North Sumatran oil palm plantations, i.e. B. cepacia, B. contaminans, B. metallica, and B. stagnalis may then be considered as potential biocontrol agents against G. boninense based on their antagonistic activities, antifungal properties, and hydrolytic enzyme activities.
format	article
author	YURNALIZA YURNALIZA DITA ISNAINI RAMBE LISTRA SARIMUNGGU MEYLISA PURBA ISNAINI NURWAHYUNI SOVIA LENNY ANISA LUTFIA ADRIAN HARTANTO
author_facet	YURNALIZA YURNALIZA DITA ISNAINI RAMBE LISTRA SARIMUNGGU MEYLISA PURBA ISNAINI NURWAHYUNI SOVIA LENNY ANISA LUTFIA ADRIAN HARTANTO
author_sort	YURNALIZA YURNALIZA
title	Screening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense
title_short	Screening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense
title_full	Screening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense
title_fullStr	Screening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense
title_full_unstemmed	Screening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense
title_sort	screening of burkholderia spp. from oil palm plantation with antagonistic properties against ganoderma boninense
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/cb310b58f80c46f7a4b6b1676221834f
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Screening of Burkholderia spp. from oil palm plantation with antagonistic properties against Ganoderma boninense

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