MicroRNA-29a promotes the proliferation of human nasal epithelial cells and inhibits their apoptosis and promotes the development of allergic rhinitis by down-regulating FOS expression.

<h4>Objective</h4>To explore the regulation of microRNA-29a (miR-29a) on FOS in human nasal epithelial cells and its molecular mechanism, as well as the effects of miR-29a on the cell proliferation and apoptosis.<h4>Methods</h4>By cell transfection, gene silencing, quantitati...

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Autores principales:	Yuqin Fan, Zhiyuan Tang, Jie Sun, Xiaorui Zhao, Zhen Li, Yiqing Zheng, Xianhai Zeng, Juan Feng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb36f5eae38f40e6985be4da1f49d449
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spelling	oai:doaj.org-article:cb36f5eae38f40e6985be4da1f49d4492021-12-02T20:18:18ZMicroRNA-29a promotes the proliferation of human nasal epithelial cells and inhibits their apoptosis and promotes the development of allergic rhinitis by down-regulating FOS expression.1932-620310.1371/journal.pone.0255480https://doaj.org/article/cb36f5eae38f40e6985be4da1f49d4492021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255480https://doaj.org/toc/1932-6203<h4>Objective</h4>To explore the regulation of microRNA-29a (miR-29a) on FOS in human nasal epithelial cells and its molecular mechanism, as well as the effects of miR-29a on the cell proliferation and apoptosis.<h4>Methods</h4>By cell transfection, gene silencing, quantitative real-time polymerase chain reaction (qRT-PCR), flow cytometry and TUNEL assay (for cell apoptosis), CCK-8 assay (for cell proliferation), dual-luciferase reporter gene assay and Western Blot, it was validated that miR-29a promoted the proliferation of human nasal epithelial cells and inhibited their apoptosis by down-regulating FOS expression in RPMI2650 and HNEpC cell lines.<h4>Results</h4>①Compared with healthy controls, miR-29a expression was up-regulated and FOS mRNA expression was down-regulated in the nasal tissues from the patients with allergic rhinitis (AR). ②MiR-29a over-expression promoted the proliferation of RPMI2650 cells and HNEpC cells but inhibited their apoptosis. ③MiR-29a targeted at FOS. ④MiR-29a over-expression and FOS silencing both significantly promoted cell proliferation and inhibited cell apoptosis. After transfection with both miR-29a and FOS, there was a decrease in the proliferation but an increase in the apoptosis of cells.⑤MiR-29a promoted the proliferation of human nasal epithelial cells and inhibited their apoptosis by down-regulating FOS expression.<h4>Conclusion</h4>MiR-29a-/FOS axis can be regarded as a potential marker and a new therapy for AR.Yuqin FanZhiyuan TangJie SunXiaorui ZhaoZhen LiYiqing ZhengXianhai ZengJuan FengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255480 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuqin Fan Zhiyuan Tang Jie Sun Xiaorui Zhao Zhen Li Yiqing Zheng Xianhai Zeng Juan Feng MicroRNA-29a promotes the proliferation of human nasal epithelial cells and inhibits their apoptosis and promotes the development of allergic rhinitis by down-regulating FOS expression.
description	<h4>Objective</h4>To explore the regulation of microRNA-29a (miR-29a) on FOS in human nasal epithelial cells and its molecular mechanism, as well as the effects of miR-29a on the cell proliferation and apoptosis.<h4>Methods</h4>By cell transfection, gene silencing, quantitative real-time polymerase chain reaction (qRT-PCR), flow cytometry and TUNEL assay (for cell apoptosis), CCK-8 assay (for cell proliferation), dual-luciferase reporter gene assay and Western Blot, it was validated that miR-29a promoted the proliferation of human nasal epithelial cells and inhibited their apoptosis by down-regulating FOS expression in RPMI2650 and HNEpC cell lines.<h4>Results</h4>①Compared with healthy controls, miR-29a expression was up-regulated and FOS mRNA expression was down-regulated in the nasal tissues from the patients with allergic rhinitis (AR). ②MiR-29a over-expression promoted the proliferation of RPMI2650 cells and HNEpC cells but inhibited their apoptosis. ③MiR-29a targeted at FOS. ④MiR-29a over-expression and FOS silencing both significantly promoted cell proliferation and inhibited cell apoptosis. After transfection with both miR-29a and FOS, there was a decrease in the proliferation but an increase in the apoptosis of cells.⑤MiR-29a promoted the proliferation of human nasal epithelial cells and inhibited their apoptosis by down-regulating FOS expression.<h4>Conclusion</h4>MiR-29a-/FOS axis can be regarded as a potential marker and a new therapy for AR.
format	article
author	Yuqin Fan Zhiyuan Tang Jie Sun Xiaorui Zhao Zhen Li Yiqing Zheng Xianhai Zeng Juan Feng
author_facet	Yuqin Fan Zhiyuan Tang Jie Sun Xiaorui Zhao Zhen Li Yiqing Zheng Xianhai Zeng Juan Feng
author_sort	Yuqin Fan
title	MicroRNA-29a promotes the proliferation of human nasal epithelial cells and inhibits their apoptosis and promotes the development of allergic rhinitis by down-regulating FOS expression.
title_short	MicroRNA-29a promotes the proliferation of human nasal epithelial cells and inhibits their apoptosis and promotes the development of allergic rhinitis by down-regulating FOS expression.
title_full	MicroRNA-29a promotes the proliferation of human nasal epithelial cells and inhibits their apoptosis and promotes the development of allergic rhinitis by down-regulating FOS expression.
title_fullStr	MicroRNA-29a promotes the proliferation of human nasal epithelial cells and inhibits their apoptosis and promotes the development of allergic rhinitis by down-regulating FOS expression.
title_full_unstemmed	MicroRNA-29a promotes the proliferation of human nasal epithelial cells and inhibits their apoptosis and promotes the development of allergic rhinitis by down-regulating FOS expression.
title_sort	microrna-29a promotes the proliferation of human nasal epithelial cells and inhibits their apoptosis and promotes the development of allergic rhinitis by down-regulating fos expression.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/cb36f5eae38f40e6985be4da1f49d449
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MicroRNA-29a promotes the proliferation of human nasal epithelial cells and inhibits their apoptosis and promotes the development of allergic rhinitis by down-regulating FOS expression.

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