Elimination of Antibiotic Resistant Surgical Implant Biofilms Using an Engineered Cationic Amphipathic Peptide WLBU2

Abstract Antibiotics are unable to remove biofilms from surgical implants. This high antibiotic tolerance is related to bacterial persisters, a sub-population of bacteria phenotypically tolerant to antibiotics secondary to a reduced metabolic state. WLBU2 is an engineered cationic amphipathic peptid...

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Autores principales:	Jonathan B. Mandell, Berthony Deslouches, Ronald C. Montelaro, Robert M. Q. Shanks, Yohei Doi, Kenneth L. Urish
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb3d0718c264403fae93484e9e97d3c0
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spelling	oai:doaj.org-article:cb3d0718c264403fae93484e9e97d3c02021-12-02T15:06:19ZElimination of Antibiotic Resistant Surgical Implant Biofilms Using an Engineered Cationic Amphipathic Peptide WLBU210.1038/s41598-017-17780-62045-2322https://doaj.org/article/cb3d0718c264403fae93484e9e97d3c02017-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-17780-6https://doaj.org/toc/2045-2322Abstract Antibiotics are unable to remove biofilms from surgical implants. This high antibiotic tolerance is related to bacterial persisters, a sub-population of bacteria phenotypically tolerant to antibiotics secondary to a reduced metabolic state. WLBU2 is an engineered cationic amphipathic peptide designed to maximize antimicrobial activity with minimal mammalian cell toxicity. The objective of this study was to test the ability of WLBU2 to remove Staphylococcus aureus surgical implant biofilms. WLBU2 effectively treated S. aureus biofilms formed by a variety of clinical MSSA and MRSA strains and created culture-negative implants in the in vitro biofilm model. Blocking bacterial metabolism by inhibiting oxidative phosphorylation did not affect WLBU2 killing compared to decreased killing by cefazolin. In the surgical implant infection animal model, WLBU2 decreased biofilm mass as compared to control, untreated samples. WLBU2 could rapidly eliminate implants in vitro and had sufficient efficacy in vivo with minimal systemic toxicity.Jonathan B. MandellBerthony DeslouchesRonald C. MontelaroRobert M. Q. ShanksYohei DoiKenneth L. UrishNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jonathan B. Mandell Berthony Deslouches Ronald C. Montelaro Robert M. Q. Shanks Yohei Doi Kenneth L. Urish Elimination of Antibiotic Resistant Surgical Implant Biofilms Using an Engineered Cationic Amphipathic Peptide WLBU2
description	Abstract Antibiotics are unable to remove biofilms from surgical implants. This high antibiotic tolerance is related to bacterial persisters, a sub-population of bacteria phenotypically tolerant to antibiotics secondary to a reduced metabolic state. WLBU2 is an engineered cationic amphipathic peptide designed to maximize antimicrobial activity with minimal mammalian cell toxicity. The objective of this study was to test the ability of WLBU2 to remove Staphylococcus aureus surgical implant biofilms. WLBU2 effectively treated S. aureus biofilms formed by a variety of clinical MSSA and MRSA strains and created culture-negative implants in the in vitro biofilm model. Blocking bacterial metabolism by inhibiting oxidative phosphorylation did not affect WLBU2 killing compared to decreased killing by cefazolin. In the surgical implant infection animal model, WLBU2 decreased biofilm mass as compared to control, untreated samples. WLBU2 could rapidly eliminate implants in vitro and had sufficient efficacy in vivo with minimal systemic toxicity.
format	article
author	Jonathan B. Mandell Berthony Deslouches Ronald C. Montelaro Robert M. Q. Shanks Yohei Doi Kenneth L. Urish
author_facet	Jonathan B. Mandell Berthony Deslouches Ronald C. Montelaro Robert M. Q. Shanks Yohei Doi Kenneth L. Urish
author_sort	Jonathan B. Mandell
title	Elimination of Antibiotic Resistant Surgical Implant Biofilms Using an Engineered Cationic Amphipathic Peptide WLBU2
title_short	Elimination of Antibiotic Resistant Surgical Implant Biofilms Using an Engineered Cationic Amphipathic Peptide WLBU2
title_full	Elimination of Antibiotic Resistant Surgical Implant Biofilms Using an Engineered Cationic Amphipathic Peptide WLBU2
title_fullStr	Elimination of Antibiotic Resistant Surgical Implant Biofilms Using an Engineered Cationic Amphipathic Peptide WLBU2
title_full_unstemmed	Elimination of Antibiotic Resistant Surgical Implant Biofilms Using an Engineered Cationic Amphipathic Peptide WLBU2
title_sort	elimination of antibiotic resistant surgical implant biofilms using an engineered cationic amphipathic peptide wlbu2
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cb3d0718c264403fae93484e9e97d3c0
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Elimination of Antibiotic Resistant Surgical Implant Biofilms Using an Engineered Cationic Amphipathic Peptide WLBU2

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