Novel Genetic Dysregulations and Oxidative Damage in Fusarium graminearum Induced by Plant Defense Eliciting Psychrophilic Bacillus atrophaeus TS1

This study elaborates inter-kingdom signaling mechanisms, presenting a sustainable and eco-friendly approach to combat biotic as well as abiotic stress in wheat. Fusarium graminearum is a devastating pathogen causing head and seedling blight in wheat, leading to huge yield and eco...

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Autores principales:	Muhammad Zubair, Ayaz Farzand, Faiza Mumtaz, Abdur Rashid Khan, Taha Majid Mahmood Sheikh, Muhammad Salman Haider, Chenjie Yu, Yujie Wang, Muhammad Ayaz, Qin Gu, Xuewen Gao, Huijun Wu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	psychrophilic biotic/abiotic stresses biochemical genetic dysregulations necrosis inducing proteins plant defense induction Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/cb41690f33be406f9c5af85f8d62889f
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spelling	oai:doaj.org-article:cb41690f33be406f9c5af85f8d62889f2021-11-25T17:53:22ZNovel Genetic Dysregulations and Oxidative Damage in <i>Fusarium graminearum</i> Induced by Plant Defense Eliciting Psychrophilic <i>Bacillus atrophaeus</i> TS110.3390/ijms2222120941422-00671661-6596https://doaj.org/article/cb41690f33be406f9c5af85f8d62889f2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12094https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067This study elaborates inter-kingdom signaling mechanisms, presenting a sustainable and eco-friendly approach to combat biotic as well as abiotic stress in wheat. <i>Fusarium graminearum</i> is a devastating pathogen causing head and seedling blight in wheat, leading to huge yield and economic losses. Psychrophilic <i>Bacillus atrophaeus</i> strain TS1 was found as a potential biocontrol agent for suppression of <i>F. graminearum</i> under low temperature by carrying out extensive biochemical and molecular studies in comparison with a temperate biocontrol model strain <i>Bacillus amyloliquefaciens</i> FZB42 at 15 and 25 °C. TS1 was able to produce hydrolytic extracellular enzymes as well as antimicrobial lipopeptides, i.e., surfactin, bacillomycin, and fengycin, efficiently at low temperatures. The <i>Bacillus</i> strain-induced oxidative cellular damage, ultrastructural deformities, and novel genetic dysregulations in the fungal pathogen as the bacterial treatment at low temperature were able to downregulate the expression of newly predicted novel fungal genes potentially belonging to necrosis inducing protein families (<i>fgHCE</i> and <i>fgNPP1</i>). The wheat pot experiments conducted at 15 and 25 °C revealed the potential of TS1 to elicit sudden induction of plant defense, namely, H<sub>2</sub>O<sub>2</sub> and callose enhanced activity of plant defense-related enzymes and induced over-expression of defense-related genes which accumulatively lead to the suppression of <i>F. graminearum</i> and decreased diseased leaf area.Muhammad ZubairAyaz FarzandFaiza MumtazAbdur Rashid KhanTaha Majid Mahmood SheikhMuhammad Salman HaiderChenjie YuYujie WangMuhammad AyazQin GuXuewen GaoHuijun WuMDPI AGarticlepsychrophilicbiotic/abiotic stressesbiochemicalgenetic dysregulationsnecrosis inducing proteinsplant defense inductionBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12094, p 12094 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	psychrophilic biotic/abiotic stresses biochemical genetic dysregulations necrosis inducing proteins plant defense induction Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	psychrophilic biotic/abiotic stresses biochemical genetic dysregulations necrosis inducing proteins plant defense induction Biology (General) QH301-705.5 Chemistry QD1-999 Muhammad Zubair Ayaz Farzand Faiza Mumtaz Abdur Rashid Khan Taha Majid Mahmood Sheikh Muhammad Salman Haider Chenjie Yu Yujie Wang Muhammad Ayaz Qin Gu Xuewen Gao Huijun Wu Novel Genetic Dysregulations and Oxidative Damage in <i>Fusarium graminearum</i> Induced by Plant Defense Eliciting Psychrophilic <i>Bacillus atrophaeus</i> TS1
description	This study elaborates inter-kingdom signaling mechanisms, presenting a sustainable and eco-friendly approach to combat biotic as well as abiotic stress in wheat. <i>Fusarium graminearum</i> is a devastating pathogen causing head and seedling blight in wheat, leading to huge yield and economic losses. Psychrophilic <i>Bacillus atrophaeus</i> strain TS1 was found as a potential biocontrol agent for suppression of <i>F. graminearum</i> under low temperature by carrying out extensive biochemical and molecular studies in comparison with a temperate biocontrol model strain <i>Bacillus amyloliquefaciens</i> FZB42 at 15 and 25 °C. TS1 was able to produce hydrolytic extracellular enzymes as well as antimicrobial lipopeptides, i.e., surfactin, bacillomycin, and fengycin, efficiently at low temperatures. The <i>Bacillus</i> strain-induced oxidative cellular damage, ultrastructural deformities, and novel genetic dysregulations in the fungal pathogen as the bacterial treatment at low temperature were able to downregulate the expression of newly predicted novel fungal genes potentially belonging to necrosis inducing protein families (<i>fgHCE</i> and <i>fgNPP1</i>). The wheat pot experiments conducted at 15 and 25 °C revealed the potential of TS1 to elicit sudden induction of plant defense, namely, H<sub>2</sub>O<sub>2</sub> and callose enhanced activity of plant defense-related enzymes and induced over-expression of defense-related genes which accumulatively lead to the suppression of <i>F. graminearum</i> and decreased diseased leaf area.
format	article
author	Muhammad Zubair Ayaz Farzand Faiza Mumtaz Abdur Rashid Khan Taha Majid Mahmood Sheikh Muhammad Salman Haider Chenjie Yu Yujie Wang Muhammad Ayaz Qin Gu Xuewen Gao Huijun Wu
author_facet	Muhammad Zubair Ayaz Farzand Faiza Mumtaz Abdur Rashid Khan Taha Majid Mahmood Sheikh Muhammad Salman Haider Chenjie Yu Yujie Wang Muhammad Ayaz Qin Gu Xuewen Gao Huijun Wu
author_sort	Muhammad Zubair
title	Novel Genetic Dysregulations and Oxidative Damage in <i>Fusarium graminearum</i> Induced by Plant Defense Eliciting Psychrophilic <i>Bacillus atrophaeus</i> TS1
title_short	Novel Genetic Dysregulations and Oxidative Damage in <i>Fusarium graminearum</i> Induced by Plant Defense Eliciting Psychrophilic <i>Bacillus atrophaeus</i> TS1
title_full	Novel Genetic Dysregulations and Oxidative Damage in <i>Fusarium graminearum</i> Induced by Plant Defense Eliciting Psychrophilic <i>Bacillus atrophaeus</i> TS1
title_fullStr	Novel Genetic Dysregulations and Oxidative Damage in <i>Fusarium graminearum</i> Induced by Plant Defense Eliciting Psychrophilic <i>Bacillus atrophaeus</i> TS1
title_full_unstemmed	Novel Genetic Dysregulations and Oxidative Damage in <i>Fusarium graminearum</i> Induced by Plant Defense Eliciting Psychrophilic <i>Bacillus atrophaeus</i> TS1
title_sort	novel genetic dysregulations and oxidative damage in <i>fusarium graminearum</i> induced by plant defense eliciting psychrophilic <i>bacillus atrophaeus</i> ts1
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cb41690f33be406f9c5af85f8d62889f
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Novel Genetic Dysregulations and Oxidative Damage in <i>Fusarium graminearum</i> Induced by Plant Defense Eliciting Psychrophilic <i>Bacillus atrophaeus</i> TS1

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