A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts

Efficient biosynthesis of lactams is still undesirable due to lacking of suitable enzyme. Here, the authors develop a sensitive transcription factor-based biosensor for high-throughput screening of marine metagenome and find a cyclase that can cyclize ω-amino fatty acids to lactam.

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Autores principales:	Soo-Jin Yeom, Moonjeong Kim, Kil Koang Kwon, Yaoyao Fu, Eugene Rha, Sung-Hyun Park, Hyewon Lee, Haseong Kim, Dae-Hee Lee, Dong-Myung Kim, Seung-Goo Lee
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/cb428eca6741429aa58b48b7daf262a2
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spelling	oai:doaj.org-article:cb428eca6741429aa58b48b7daf262a22021-12-02T16:49:49ZA synthetic microbial biosensor for high-throughput screening of lactam biocatalysts10.1038/s41467-018-07488-02041-1723https://doaj.org/article/cb428eca6741429aa58b48b7daf262a22018-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-07488-0https://doaj.org/toc/2041-1723Efficient biosynthesis of lactams is still undesirable due to lacking of suitable enzyme. Here, the authors develop a sensitive transcription factor-based biosensor for high-throughput screening of marine metagenome and find a cyclase that can cyclize ω-amino fatty acids to lactam.Soo-Jin YeomMoonjeong KimKil Koang KwonYaoyao FuEugene RhaSung-Hyun ParkHyewon LeeHaseong KimDae-Hee LeeDong-Myung KimSeung-Goo LeeNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Soo-Jin Yeom Moonjeong Kim Kil Koang Kwon Yaoyao Fu Eugene Rha Sung-Hyun Park Hyewon Lee Haseong Kim Dae-Hee Lee Dong-Myung Kim Seung-Goo Lee A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts
description	Efficient biosynthesis of lactams is still undesirable due to lacking of suitable enzyme. Here, the authors develop a sensitive transcription factor-based biosensor for high-throughput screening of marine metagenome and find a cyclase that can cyclize ω-amino fatty acids to lactam.
format	article
author	Soo-Jin Yeom Moonjeong Kim Kil Koang Kwon Yaoyao Fu Eugene Rha Sung-Hyun Park Hyewon Lee Haseong Kim Dae-Hee Lee Dong-Myung Kim Seung-Goo Lee
author_facet	Soo-Jin Yeom Moonjeong Kim Kil Koang Kwon Yaoyao Fu Eugene Rha Sung-Hyun Park Hyewon Lee Haseong Kim Dae-Hee Lee Dong-Myung Kim Seung-Goo Lee
author_sort	Soo-Jin Yeom
title	A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts
title_short	A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts
title_full	A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts
title_fullStr	A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts
title_full_unstemmed	A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts
title_sort	synthetic microbial biosensor for high-throughput screening of lactam biocatalysts
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/cb428eca6741429aa58b48b7daf262a2
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A synthetic microbial biosensor for high-throughput screening of lactam biocatalysts

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