An ICCD camera-based time-domain ultrasound-switchable fluorescence imaging system

Abstract Fluorescence imaging in centimeter-deep tissues with high resolution is highly desirable for many biomedical applications. Recently, we have developed a new imaging modality, ultrasound-switchable fluorescence (USF) imaging, for achieving this goal. In our previous work, we successfully ach...

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Autores principales:	Shuai Yu, Tingfeng Yao, Baohong Yuan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb43e9be55794437be935b9175190ccb
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spelling	oai:doaj.org-article:cb43e9be55794437be935b9175190ccb2021-12-02T15:09:56ZAn ICCD camera-based time-domain ultrasound-switchable fluorescence imaging system10.1038/s41598-019-47156-x2045-2322https://doaj.org/article/cb43e9be55794437be935b9175190ccb2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-47156-xhttps://doaj.org/toc/2045-2322Abstract Fluorescence imaging in centimeter-deep tissues with high resolution is highly desirable for many biomedical applications. Recently, we have developed a new imaging modality, ultrasound-switchable fluorescence (USF) imaging, for achieving this goal. In our previous work, we successfully achieved USF imaging with several types of USF contrast agents and imaging systems. In this study, we introduced a new USF imaging system: an intensified charge-coupled device (ICCD) camera-based, time-domain USF imaging system. We demonstrated the principle of time-domain USF imaging by using two USF contrast agents. With a series of USF imaging experiments, we demonstrated the tradeoffs among different experimental parameters (i.e., data acquisition time, including CCD camera recording time and intensifier gate delay; focused ultrasound (FU) power; and imaging depth) and the image qualities (i.e., signal-to-noise ratio, spatial resolution, and temporal resolution). In this study, we also discussed several imaging strategies for achieving a high-quality USF image via this time-domain system.Shuai YuTingfeng YaoBaohong YuanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shuai Yu Tingfeng Yao Baohong Yuan An ICCD camera-based time-domain ultrasound-switchable fluorescence imaging system
description	Abstract Fluorescence imaging in centimeter-deep tissues with high resolution is highly desirable for many biomedical applications. Recently, we have developed a new imaging modality, ultrasound-switchable fluorescence (USF) imaging, for achieving this goal. In our previous work, we successfully achieved USF imaging with several types of USF contrast agents and imaging systems. In this study, we introduced a new USF imaging system: an intensified charge-coupled device (ICCD) camera-based, time-domain USF imaging system. We demonstrated the principle of time-domain USF imaging by using two USF contrast agents. With a series of USF imaging experiments, we demonstrated the tradeoffs among different experimental parameters (i.e., data acquisition time, including CCD camera recording time and intensifier gate delay; focused ultrasound (FU) power; and imaging depth) and the image qualities (i.e., signal-to-noise ratio, spatial resolution, and temporal resolution). In this study, we also discussed several imaging strategies for achieving a high-quality USF image via this time-domain system.
format	article
author	Shuai Yu Tingfeng Yao Baohong Yuan
author_facet	Shuai Yu Tingfeng Yao Baohong Yuan
author_sort	Shuai Yu
title	An ICCD camera-based time-domain ultrasound-switchable fluorescence imaging system
title_short	An ICCD camera-based time-domain ultrasound-switchable fluorescence imaging system
title_full	An ICCD camera-based time-domain ultrasound-switchable fluorescence imaging system
title_fullStr	An ICCD camera-based time-domain ultrasound-switchable fluorescence imaging system
title_full_unstemmed	An ICCD camera-based time-domain ultrasound-switchable fluorescence imaging system
title_sort	iccd camera-based time-domain ultrasound-switchable fluorescence imaging system
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/cb43e9be55794437be935b9175190ccb
work_keys_str_mv	AT shuaiyu aniccdcamerabasedtimedomainultrasoundswitchablefluorescenceimagingsystem AT tingfengyao aniccdcamerabasedtimedomainultrasoundswitchablefluorescenceimagingsystem AT baohongyuan aniccdcamerabasedtimedomainultrasoundswitchablefluorescenceimagingsystem AT shuaiyu iccdcamerabasedtimedomainultrasoundswitchablefluorescenceimagingsystem AT tingfengyao iccdcamerabasedtimedomainultrasoundswitchablefluorescenceimagingsystem AT baohongyuan iccdcamerabasedtimedomainultrasoundswitchablefluorescenceimagingsystem
_version_	1718387779492118528

An ICCD camera-based time-domain ultrasound-switchable fluorescence imaging system

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