Isolation, characterization and optimization of chrysene degradation using bacteria isolated from oil-contaminated water

Polyaromatic hydrocarbons (PAHs) are uncharged, non-polar molecules generated from natural and anthropogenic activities, where the emissions from anthropogenic activities predominate. Chrysene is a high molecular weight PAH, which is found to be highly recalcitrant and mutagenic in nature. The aim o...

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Autores principales:	Smeera Thomas, Nitha Thalakkale Veettil, Kavitha Subbiah
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	bacillus halotolerans biodegradation chrysene design expert poly aromatic hydrocarbon 16s rrna sequencing Environmental technology. Sanitary engineering TD1-1066
Acceso en línea:	https://doaj.org/article/cb4a187e93614dfa90269ee5cce38a71
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spelling	oai:doaj.org-article:cb4a187e93614dfa90269ee5cce38a712021-12-02T07:40:13ZIsolation, characterization and optimization of chrysene degradation using bacteria isolated from oil-contaminated water0273-12231996-973210.2166/wst.2021.227https://doaj.org/article/cb4a187e93614dfa90269ee5cce38a712021-11-01T00:00:00Zhttp://wst.iwaponline.com/content/84/10-11/2737https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732Polyaromatic hydrocarbons (PAHs) are uncharged, non-polar molecules generated from natural and anthropogenic activities, where the emissions from anthropogenic activities predominate. Chrysene is a high molecular weight PAH, which is found to be highly recalcitrant and mutagenic in nature. The aim of this study was to isolate chrysene-degrading microorganisms from oil-contaminated water and to enhance their degradative conditions using design expert. From the various samples collected, 19 bacterial strains were obtained through enrichment culture and the one which showed highest activity was identified by 16S rRNA sequencing as Bacillus halotolerans. Under optimum conditions of 100 mg/L chrysene concentration, 1,000 mg/L nitrogen source, and pH 6, B. halotolerans exhibited 90% chrysene degradation on sixth day. Positive results for the enzymes laccase and catechol 1,2 dioxygenase confirmed the ability for chrysene degradation by the isolated strain. Major metabolic intermediate determined in gas chromatography-mass spectrometry (GCMS) analysis was diisooctyl phthalate. Hence it can be concluded that B. halotolerans can be a promising candidate for the removal of high molecular weight (HMW) hydrocarbons from contaminated environments. HIGHLIGHTS Bacterial strain capable of degrading chrysene was isolated and sequenced.; Operational conditions like carbon source, nitrogen, pH and incubation period were optimized using design expert software to ensure maximum degradation.; It has been demonstrated that Bacillus halotolerans can mineralize chrysene to fewer toxic forms like catechol and phthalic acid derivatives.;Smeera ThomasNitha Thalakkale VeettilKavitha SubbiahIWA Publishingarticlebacillus halotoleransbiodegradationchrysenedesign expertpoly aromatic hydrocarbon16s rrna sequencingEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 84, Iss 10-11, Pp 2737-2748 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacillus halotolerans biodegradation chrysene design expert poly aromatic hydrocarbon 16s rrna sequencing Environmental technology. Sanitary engineering TD1-1066
spellingShingle	bacillus halotolerans biodegradation chrysene design expert poly aromatic hydrocarbon 16s rrna sequencing Environmental technology. Sanitary engineering TD1-1066 Smeera Thomas Nitha Thalakkale Veettil Kavitha Subbiah Isolation, characterization and optimization of chrysene degradation using bacteria isolated from oil-contaminated water
description	Polyaromatic hydrocarbons (PAHs) are uncharged, non-polar molecules generated from natural and anthropogenic activities, where the emissions from anthropogenic activities predominate. Chrysene is a high molecular weight PAH, which is found to be highly recalcitrant and mutagenic in nature. The aim of this study was to isolate chrysene-degrading microorganisms from oil-contaminated water and to enhance their degradative conditions using design expert. From the various samples collected, 19 bacterial strains were obtained through enrichment culture and the one which showed highest activity was identified by 16S rRNA sequencing as Bacillus halotolerans. Under optimum conditions of 100 mg/L chrysene concentration, 1,000 mg/L nitrogen source, and pH 6, B. halotolerans exhibited 90% chrysene degradation on sixth day. Positive results for the enzymes laccase and catechol 1,2 dioxygenase confirmed the ability for chrysene degradation by the isolated strain. Major metabolic intermediate determined in gas chromatography-mass spectrometry (GCMS) analysis was diisooctyl phthalate. Hence it can be concluded that B. halotolerans can be a promising candidate for the removal of high molecular weight (HMW) hydrocarbons from contaminated environments. HIGHLIGHTS Bacterial strain capable of degrading chrysene was isolated and sequenced.; Operational conditions like carbon source, nitrogen, pH and incubation period were optimized using design expert software to ensure maximum degradation.; It has been demonstrated that Bacillus halotolerans can mineralize chrysene to fewer toxic forms like catechol and phthalic acid derivatives.;
format	article
author	Smeera Thomas Nitha Thalakkale Veettil Kavitha Subbiah
author_facet	Smeera Thomas Nitha Thalakkale Veettil Kavitha Subbiah
author_sort	Smeera Thomas
title	Isolation, characterization and optimization of chrysene degradation using bacteria isolated from oil-contaminated water
title_short	Isolation, characterization and optimization of chrysene degradation using bacteria isolated from oil-contaminated water
title_full	Isolation, characterization and optimization of chrysene degradation using bacteria isolated from oil-contaminated water
title_fullStr	Isolation, characterization and optimization of chrysene degradation using bacteria isolated from oil-contaminated water
title_full_unstemmed	Isolation, characterization and optimization of chrysene degradation using bacteria isolated from oil-contaminated water
title_sort	isolation, characterization and optimization of chrysene degradation using bacteria isolated from oil-contaminated water
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/cb4a187e93614dfa90269ee5cce38a71
work_keys_str_mv	AT smeerathomas isolationcharacterizationandoptimizationofchrysenedegradationusingbacteriaisolatedfromoilcontaminatedwater AT nithathalakkaleveettil isolationcharacterizationandoptimizationofchrysenedegradationusingbacteriaisolatedfromoilcontaminatedwater AT kavithasubbiah isolationcharacterizationandoptimizationofchrysenedegradationusingbacteriaisolatedfromoilcontaminatedwater
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Isolation, characterization and optimization of chrysene degradation using bacteria isolated from oil-contaminated water

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