Effects of Dietary Modified Bazhen on Reproductive Performance, Immunity, Breast Milk Microbes, and Metabolome Characterization of Sows

Bazhen is a classic prescription used for the prevention of qi and blood deficiency. The present study aimed to investigate the effects of dietary supplementation with modified Bazhen powder (MBP) on sows during lactation. Forty pure-bred Yorkshire sows on day 100 of gestation were randomly fed a st...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jian Geng, Weicheng Jin, Jingyou Hao, Mohan Huo, Yuefeng Zhang, Chunmei Xie, Baokai Zhao, Yanhua Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	modified Bazhen powder reproductive performance milk microbes metabolome characterization sow Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/cb4a4b4d640b4e59b8efe54423e4c083
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cb4a4b4d640b4e59b8efe54423e4c083
record_format	dspace
spelling	oai:doaj.org-article:cb4a4b4d640b4e59b8efe54423e4c0832021-11-15T06:52:42ZEffects of Dietary Modified Bazhen on Reproductive Performance, Immunity, Breast Milk Microbes, and Metabolome Characterization of Sows1664-302X10.3389/fmicb.2021.758224https://doaj.org/article/cb4a4b4d640b4e59b8efe54423e4c0832021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.758224/fullhttps://doaj.org/toc/1664-302XBazhen is a classic prescription used for the prevention of qi and blood deficiency. The present study aimed to investigate the effects of dietary supplementation with modified Bazhen powder (MBP) on sows during lactation. Forty pure-bred Yorkshire sows on day 100 of gestation were randomly fed a standard diet supplemented with 20 g MBP per sow per day (MBP group) or without (control group) during -14 to 7 days relative to parturition. Results showed that the serum levels of interleukin 2 (IL-2), immunoglobulin A (IgA), and IgG were higher, whereas IL-10 level was lower in sows fed with MBP diet than in controls on day 7 postpartum. A significantly elevated proportion of serum CD4+ T cells and a slight increase in the ratio of CD4+ to CD8+ T cells in the MBP group were also observed. Furthermore, MBP supplementation improved gastrointestinal function of postpartum sows, evidenced by increased levels of motilin, gastrin, and nitric oxide. Ultra high-performance liquid chromatography combined with a quadrupole time of flight and tandem mass spectrometer identified a total of 21 absorbed milk components. 16S rRNA gene amplicon sequencing data revealed that the microbiota diversity of the colostrum and transitional milk in the MBP group was increased. At the genus level, relative abundances of Enterococcus and Anaerostipes were significantly lower in the MBP group on day 0 of lactation. Metabolomic analysis showed that 38 metabolites were upregulated, and 41 metabolites were downregulated in the transitional milk; 31 metabolites were upregulated and 8 metabolites were downregulated in the colostrum in response to MBP. Metabolic pathways, protein digestion and absorption, and biosynthesis of amino acids were enriched in the colostrum and transitional milk. Our findings provide new insights into the beneficial effects of MBP, highlighted by the changes to the microbiota and metabolomic profile of breast milk from sows fed with an MBP-supplemented diet. Thus, MBP should be considered as a potential dietary supplement for lactating sows in pork production.Jian GengJian GengWeicheng JinWeicheng JinJingyou HaoMohan HuoYuefeng ZhangYuefeng ZhangChunmei XieChunmei XieBaokai ZhaoYanhua LiYanhua LiYanhua LiFrontiers Media S.A.articlemodified Bazhen powderreproductive performancemilk microbesmetabolome characterizationsowMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	modified Bazhen powder reproductive performance milk microbes metabolome characterization sow Microbiology QR1-502
spellingShingle	modified Bazhen powder reproductive performance milk microbes metabolome characterization sow Microbiology QR1-502 Jian Geng Jian Geng Weicheng Jin Weicheng Jin Jingyou Hao Mohan Huo Yuefeng Zhang Yuefeng Zhang Chunmei Xie Chunmei Xie Baokai Zhao Yanhua Li Yanhua Li Yanhua Li Effects of Dietary Modified Bazhen on Reproductive Performance, Immunity, Breast Milk Microbes, and Metabolome Characterization of Sows
description	Bazhen is a classic prescription used for the prevention of qi and blood deficiency. The present study aimed to investigate the effects of dietary supplementation with modified Bazhen powder (MBP) on sows during lactation. Forty pure-bred Yorkshire sows on day 100 of gestation were randomly fed a standard diet supplemented with 20 g MBP per sow per day (MBP group) or without (control group) during -14 to 7 days relative to parturition. Results showed that the serum levels of interleukin 2 (IL-2), immunoglobulin A (IgA), and IgG were higher, whereas IL-10 level was lower in sows fed with MBP diet than in controls on day 7 postpartum. A significantly elevated proportion of serum CD4+ T cells and a slight increase in the ratio of CD4+ to CD8+ T cells in the MBP group were also observed. Furthermore, MBP supplementation improved gastrointestinal function of postpartum sows, evidenced by increased levels of motilin, gastrin, and nitric oxide. Ultra high-performance liquid chromatography combined with a quadrupole time of flight and tandem mass spectrometer identified a total of 21 absorbed milk components. 16S rRNA gene amplicon sequencing data revealed that the microbiota diversity of the colostrum and transitional milk in the MBP group was increased. At the genus level, relative abundances of Enterococcus and Anaerostipes were significantly lower in the MBP group on day 0 of lactation. Metabolomic analysis showed that 38 metabolites were upregulated, and 41 metabolites were downregulated in the transitional milk; 31 metabolites were upregulated and 8 metabolites were downregulated in the colostrum in response to MBP. Metabolic pathways, protein digestion and absorption, and biosynthesis of amino acids were enriched in the colostrum and transitional milk. Our findings provide new insights into the beneficial effects of MBP, highlighted by the changes to the microbiota and metabolomic profile of breast milk from sows fed with an MBP-supplemented diet. Thus, MBP should be considered as a potential dietary supplement for lactating sows in pork production.
format	article
author	Jian Geng Jian Geng Weicheng Jin Weicheng Jin Jingyou Hao Mohan Huo Yuefeng Zhang Yuefeng Zhang Chunmei Xie Chunmei Xie Baokai Zhao Yanhua Li Yanhua Li Yanhua Li
author_facet	Jian Geng Jian Geng Weicheng Jin Weicheng Jin Jingyou Hao Mohan Huo Yuefeng Zhang Yuefeng Zhang Chunmei Xie Chunmei Xie Baokai Zhao Yanhua Li Yanhua Li Yanhua Li
author_sort	Jian Geng
title	Effects of Dietary Modified Bazhen on Reproductive Performance, Immunity, Breast Milk Microbes, and Metabolome Characterization of Sows
title_short	Effects of Dietary Modified Bazhen on Reproductive Performance, Immunity, Breast Milk Microbes, and Metabolome Characterization of Sows
title_full	Effects of Dietary Modified Bazhen on Reproductive Performance, Immunity, Breast Milk Microbes, and Metabolome Characterization of Sows
title_fullStr	Effects of Dietary Modified Bazhen on Reproductive Performance, Immunity, Breast Milk Microbes, and Metabolome Characterization of Sows
title_full_unstemmed	Effects of Dietary Modified Bazhen on Reproductive Performance, Immunity, Breast Milk Microbes, and Metabolome Characterization of Sows
title_sort	effects of dietary modified bazhen on reproductive performance, immunity, breast milk microbes, and metabolome characterization of sows
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/cb4a4b4d640b4e59b8efe54423e4c083
work_keys_str_mv	AT jiangeng effectsofdietarymodifiedbazhenonreproductiveperformanceimmunitybreastmilkmicrobesandmetabolomecharacterizationofsows AT jiangeng effectsofdietarymodifiedbazhenonreproductiveperformanceimmunitybreastmilkmicrobesandmetabolomecharacterizationofsows AT weichengjin effectsofdietarymodifiedbazhenonreproductiveperformanceimmunitybreastmilkmicrobesandmetabolomecharacterizationofsows AT weichengjin effectsofdietarymodifiedbazhenonreproductiveperformanceimmunitybreastmilkmicrobesandmetabolomecharacterizationofsows AT jingyouhao effectsofdietarymodifiedbazhenonreproductiveperformanceimmunitybreastmilkmicrobesandmetabolomecharacterizationofsows AT mohanhuo effectsofdietarymodifiedbazhenonreproductiveperformanceimmunitybreastmilkmicrobesandmetabolomecharacterizationofsows AT yuefengzhang effectsofdietarymodifiedbazhenonreproductiveperformanceimmunitybreastmilkmicrobesandmetabolomecharacterizationofsows AT yuefengzhang effectsofdietarymodifiedbazhenonreproductiveperformanceimmunitybreastmilkmicrobesandmetabolomecharacterizationofsows AT chunmeixie effectsofdietarymodifiedbazhenonreproductiveperformanceimmunitybreastmilkmicrobesandmetabolomecharacterizationofsows AT chunmeixie effectsofdietarymodifiedbazhenonreproductiveperformanceimmunitybreastmilkmicrobesandmetabolomecharacterizationofsows AT baokaizhao effectsofdietarymodifiedbazhenonreproductiveperformanceimmunitybreastmilkmicrobesandmetabolomecharacterizationofsows AT yanhuali effectsofdietarymodifiedbazhenonreproductiveperformanceimmunitybreastmilkmicrobesandmetabolomecharacterizationofsows AT yanhuali effectsofdietarymodifiedbazhenonreproductiveperformanceimmunitybreastmilkmicrobesandmetabolomecharacterizationofsows AT yanhuali effectsofdietarymodifiedbazhenonreproductiveperformanceimmunitybreastmilkmicrobesandmetabolomecharacterizationofsows
_version_	1718428581191745536

Effects of Dietary Modified Bazhen on Reproductive Performance, Immunity, Breast Milk Microbes, and Metabolome Characterization of Sows

Ejemplares similares