Biocompatibility of Fe3O4@Au composite magnetic nanoparticles in vitro and in vivo

Yuntao Li1,2, Jing Liu1, Yuejiao Zhong3, Jia Zhang1, Ziyu Wang1, Li Wang1, Yanli An1, Mei Lin1, Zhiqiang Gao2, Dongsheng Zhang11School of Medicine, Southeast University, Nanjing, Jiangsu Province, People&#39;s Republic of China; 2Second Affiliated Hospital of Nanjing Medical University, Nanj...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Li Y, Liu J, Zhong Y, Zhang J, Wang Z, Wang L, An Y, Lin M, Gao Z, Zhang D
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/cb4fcdd881d44457a2af1a1e81e0e40b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cb4fcdd881d44457a2af1a1e81e0e40b
record_format	dspace
spelling	oai:doaj.org-article:cb4fcdd881d44457a2af1a1e81e0e40b2021-12-02T01:32:46ZBiocompatibility of Fe3O4@Au composite magnetic nanoparticles in vitro and in vivo1176-91141178-2013https://doaj.org/article/cb4fcdd881d44457a2af1a1e81e0e40b2011-11-01T00:00:00Zhttp://www.dovepress.com/biocompatibility-of-fe3o4au-composite-magnetic-nanoparticles-in-vitro--a8634https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yuntao Li1,2, Jing Liu1, Yuejiao Zhong3, Jia Zhang1, Ziyu Wang1, Li Wang1, Yanli An1, Mei Lin1, Zhiqiang Gao2, Dongsheng Zhang11School of Medicine, Southeast University, Nanjing, Jiangsu Province, People&#39;s Republic of China; 2Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, People&#39;s Republic of China; 3Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research, Nanjing, Jiangsu Province, People&#39;s Republic of ChinaPurpose: This research was conducted to assess the biocompatibility of the core-shell Fe3O4@Au composite magnetic nanoparticles (MNPs), which have potential application in tumor hyperthermia. Methods: Fe3O4@Au composite MNPs with core-shell structure were synthesized by reduction of Au3+ in the presence of Fe3O4-MNPs prepared by improved co-precipitation. Cytotoxicity assay, hemolysis test, micronucleus (MN) assay, and detection of acute toxicity in mice and beagle dogs were then carried out.Results: The result of cytotoxicity assay showed that the toxicity grade of this material on mouse fibroblast cell line (L-929) was classified as grade 1, which belongs to no cytotoxicity. Hemolysis rates showed 0.278%, 0.232%, and 0.197%, far less than 5%, after treatment with different concentrations of Fe3O4@Au composite MNPs. In the MN assay, there was no significant difference in MN formation rates between the experimental groups and negative control (P &gt; 0.05), but there was a significant difference between the experimental groups and the positive control (P &lt; 0.05). The median lethal dose of the Fe3O4@Au composite MNPs after intraperitoneal administration in mice was 8.39 g/kg, and the 95% confidence interval was 6.58-10.72 g/kg, suggesting that these nanoparticles have a wide safety margin. Acute toxicity testing in beagle dogs also showed no significant difference in body weight between the treatment groups at 1, 2, 3, and 4 weeks after liver injection and no behavioral changes. Furthermore, blood parameters, autopsy, and histopathological studies in the experimental group showed no significant difference compared with the control group.Conclusion: The results indicate that Fe3O4@Au composite MNPs appear to be highly biocompatible and safe nanoparticles that are suitable for further application in tumor hyperthermia. Keywords: toxicity, hyperthermia, core-shellLi YLiu JZhong YZhang JWang ZWang LAn YLin MGao ZZhang DDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2805-2819 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Li Y Liu J Zhong Y Zhang J Wang Z Wang L An Y Lin M Gao Z Zhang D Biocompatibility of Fe3O4@Au composite magnetic nanoparticles in vitro and in vivo
description	Yuntao Li1,2, Jing Liu1, Yuejiao Zhong3, Jia Zhang1, Ziyu Wang1, Li Wang1, Yanli An1, Mei Lin1, Zhiqiang Gao2, Dongsheng Zhang11School of Medicine, Southeast University, Nanjing, Jiangsu Province, People&#39;s Republic of China; 2Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu Province, People&#39;s Republic of China; 3Jiangsu Cancer Hospital and Jiangsu Institute of Cancer Research, Nanjing, Jiangsu Province, People&#39;s Republic of ChinaPurpose: This research was conducted to assess the biocompatibility of the core-shell Fe3O4@Au composite magnetic nanoparticles (MNPs), which have potential application in tumor hyperthermia. Methods: Fe3O4@Au composite MNPs with core-shell structure were synthesized by reduction of Au3+ in the presence of Fe3O4-MNPs prepared by improved co-precipitation. Cytotoxicity assay, hemolysis test, micronucleus (MN) assay, and detection of acute toxicity in mice and beagle dogs were then carried out.Results: The result of cytotoxicity assay showed that the toxicity grade of this material on mouse fibroblast cell line (L-929) was classified as grade 1, which belongs to no cytotoxicity. Hemolysis rates showed 0.278%, 0.232%, and 0.197%, far less than 5%, after treatment with different concentrations of Fe3O4@Au composite MNPs. In the MN assay, there was no significant difference in MN formation rates between the experimental groups and negative control (P &gt; 0.05), but there was a significant difference between the experimental groups and the positive control (P &lt; 0.05). The median lethal dose of the Fe3O4@Au composite MNPs after intraperitoneal administration in mice was 8.39 g/kg, and the 95% confidence interval was 6.58-10.72 g/kg, suggesting that these nanoparticles have a wide safety margin. Acute toxicity testing in beagle dogs also showed no significant difference in body weight between the treatment groups at 1, 2, 3, and 4 weeks after liver injection and no behavioral changes. Furthermore, blood parameters, autopsy, and histopathological studies in the experimental group showed no significant difference compared with the control group.Conclusion: The results indicate that Fe3O4@Au composite MNPs appear to be highly biocompatible and safe nanoparticles that are suitable for further application in tumor hyperthermia. Keywords: toxicity, hyperthermia, core-shell
format	article
author	Li Y Liu J Zhong Y Zhang J Wang Z Wang L An Y Lin M Gao Z Zhang D
author_facet	Li Y Liu J Zhong Y Zhang J Wang Z Wang L An Y Lin M Gao Z Zhang D
author_sort	Li Y
title	Biocompatibility of Fe3O4@Au composite magnetic nanoparticles in vitro and in vivo
title_short	Biocompatibility of Fe3O4@Au composite magnetic nanoparticles in vitro and in vivo
title_full	Biocompatibility of Fe3O4@Au composite magnetic nanoparticles in vitro and in vivo
title_fullStr	Biocompatibility of Fe3O4@Au composite magnetic nanoparticles in vitro and in vivo
title_full_unstemmed	Biocompatibility of Fe3O4@Au composite magnetic nanoparticles in vitro and in vivo
title_sort	biocompatibility of fe3o4@au composite magnetic nanoparticles in vitro and in vivo
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/cb4fcdd881d44457a2af1a1e81e0e40b
work_keys_str_mv	AT liy biocompatibilityoffe3o4aucompositemagneticnanoparticlesinvitroandinvivo AT liuj biocompatibilityoffe3o4aucompositemagneticnanoparticlesinvitroandinvivo AT zhongy biocompatibilityoffe3o4aucompositemagneticnanoparticlesinvitroandinvivo AT zhangj biocompatibilityoffe3o4aucompositemagneticnanoparticlesinvitroandinvivo AT wangz biocompatibilityoffe3o4aucompositemagneticnanoparticlesinvitroandinvivo AT wangl biocompatibilityoffe3o4aucompositemagneticnanoparticlesinvitroandinvivo AT any biocompatibilityoffe3o4aucompositemagneticnanoparticlesinvitroandinvivo AT linm biocompatibilityoffe3o4aucompositemagneticnanoparticlesinvitroandinvivo AT gaoz biocompatibilityoffe3o4aucompositemagneticnanoparticlesinvitroandinvivo AT zhangd biocompatibilityoffe3o4aucompositemagneticnanoparticlesinvitroandinvivo
_version_	1718403040391725056

Biocompatibility of Fe3O4@Au composite magnetic nanoparticles in vitro and in vivo

Ejemplares similares