Timescale of hole closure during plasma membrane repair estimated by calcium imaging and numerical modeling

Abstract Plasma membrane repair is essential for eukaryotic cell life and is triggered by the influx of calcium through membrane wounds. Repair consists of sequential steps, with closure of the membrane hole being the key event that allows the cell to recover, thus identifying the kinetics of hole c...

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Autores principales:	Martin Berg Klenow, Anne Sofie Busk Heitmann, Jesper Nylandsted, Adam Cohen Simonsen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb50f1d96543458da9d5ea6e6d198add
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spelling	oai:doaj.org-article:cb50f1d96543458da9d5ea6e6d198add2021-12-02T12:11:28ZTimescale of hole closure during plasma membrane repair estimated by calcium imaging and numerical modeling10.1038/s41598-021-82926-62045-2322https://doaj.org/article/cb50f1d96543458da9d5ea6e6d198add2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82926-6https://doaj.org/toc/2045-2322Abstract Plasma membrane repair is essential for eukaryotic cell life and is triggered by the influx of calcium through membrane wounds. Repair consists of sequential steps, with closure of the membrane hole being the key event that allows the cell to recover, thus identifying the kinetics of hole closure as important for clarifying repair mechanisms and as a quantitative handle on repair efficiency. We implement calcium imaging in MCF7 breast carcinoma cells subject to laser damage, coupled with a model describing the spatio-temporal calcium distribution. The model identifies the time point of hole closure as the time of maximum calcium signal. Analysis of cell data estimates the closure time as: $$\langle t_c \rangle =5.45\pm 2.25$$ ⟨ t c ⟩ = 5.45 ± 2.25 s and $$\langle t_c \rangle =6.81\pm 4.69$$ ⟨ t c ⟩ = 6.81 ± 4.69 s using GCaMP6s-CAAX and GCaMP6s probes respectively. The timescale was confirmed by independent time-lapse imaging of a hole during sealing. Moreover, the analysis estimates the characteristic time scale of calcium removal, the penetration depth of the calcium wave and the diffusion coefficient. Probing of hole closure times emerges as a strong universal tool for quantification of plasma membrane repairMartin Berg KlenowAnne Sofie Busk HeitmannJesper NylandstedAdam Cohen SimonsenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Martin Berg Klenow Anne Sofie Busk Heitmann Jesper Nylandsted Adam Cohen Simonsen Timescale of hole closure during plasma membrane repair estimated by calcium imaging and numerical modeling
description	Abstract Plasma membrane repair is essential for eukaryotic cell life and is triggered by the influx of calcium through membrane wounds. Repair consists of sequential steps, with closure of the membrane hole being the key event that allows the cell to recover, thus identifying the kinetics of hole closure as important for clarifying repair mechanisms and as a quantitative handle on repair efficiency. We implement calcium imaging in MCF7 breast carcinoma cells subject to laser damage, coupled with a model describing the spatio-temporal calcium distribution. The model identifies the time point of hole closure as the time of maximum calcium signal. Analysis of cell data estimates the closure time as: $$\langle t_c \rangle =5.45\pm 2.25$$ ⟨ t c ⟩ = 5.45 ± 2.25 s and $$\langle t_c \rangle =6.81\pm 4.69$$ ⟨ t c ⟩ = 6.81 ± 4.69 s using GCaMP6s-CAAX and GCaMP6s probes respectively. The timescale was confirmed by independent time-lapse imaging of a hole during sealing. Moreover, the analysis estimates the characteristic time scale of calcium removal, the penetration depth of the calcium wave and the diffusion coefficient. Probing of hole closure times emerges as a strong universal tool for quantification of plasma membrane repair
format	article
author	Martin Berg Klenow Anne Sofie Busk Heitmann Jesper Nylandsted Adam Cohen Simonsen
author_facet	Martin Berg Klenow Anne Sofie Busk Heitmann Jesper Nylandsted Adam Cohen Simonsen
author_sort	Martin Berg Klenow
title	Timescale of hole closure during plasma membrane repair estimated by calcium imaging and numerical modeling
title_short	Timescale of hole closure during plasma membrane repair estimated by calcium imaging and numerical modeling
title_full	Timescale of hole closure during plasma membrane repair estimated by calcium imaging and numerical modeling
title_fullStr	Timescale of hole closure during plasma membrane repair estimated by calcium imaging and numerical modeling
title_full_unstemmed	Timescale of hole closure during plasma membrane repair estimated by calcium imaging and numerical modeling
title_sort	timescale of hole closure during plasma membrane repair estimated by calcium imaging and numerical modeling
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cb50f1d96543458da9d5ea6e6d198add
work_keys_str_mv	AT martinbergklenow timescaleofholeclosureduringplasmamembranerepairestimatedbycalciumimagingandnumericalmodeling AT annesofiebuskheitmann timescaleofholeclosureduringplasmamembranerepairestimatedbycalciumimagingandnumericalmodeling AT jespernylandsted timescaleofholeclosureduringplasmamembranerepairestimatedbycalciumimagingandnumericalmodeling AT adamcohensimonsen timescaleofholeclosureduringplasmamembranerepairestimatedbycalciumimagingandnumericalmodeling
_version_	1718394657702936576

Timescale of hole closure during plasma membrane repair estimated by calcium imaging and numerical modeling

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