Genomic signatures of Lake Erie bacteria suggest interaction in the Microcystis phycosphere.
Microbial interactions in harmful algal bloom (HAB) communities have been examined in marine systems, but are poorly studied in fresh waters. To investigate HAB-microbe interactions, we isolated bacteria with close associations to bloom-forming cyanobacteria, Microcystis spp., during a 2017 bloom in...
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2021
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oai:doaj.org-article:cb519f536fe34085a0bc637a3ae834292021-12-02T20:14:18ZGenomic signatures of Lake Erie bacteria suggest interaction in the Microcystis phycosphere.1932-620310.1371/journal.pone.0257017https://doaj.org/article/cb519f536fe34085a0bc637a3ae834292021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257017https://doaj.org/toc/1932-6203Microbial interactions in harmful algal bloom (HAB) communities have been examined in marine systems, but are poorly studied in fresh waters. To investigate HAB-microbe interactions, we isolated bacteria with close associations to bloom-forming cyanobacteria, Microcystis spp., during a 2017 bloom in the western basin of Lake Erie. The genomes of five isolates (Exiguobacterium sp. JMULE1, Enterobacter sp. JMULE2, Deinococcus sp. JMULE3, Paenibacillus sp. JMULE4, and Acidovorax sp. JMULE5.) were sequenced on a PacBio Sequel system. These genomes ranged in size from 3.1 Mbp (Exiguobacterium sp. JMULE1) to 5.7 Mbp (Enterobacter sp. JMULE2). The genomes were analyzed for genes relating to critical metabolic functions, including nitrogen reduction and carbon utilization. All five of the sequenced genomes contained genes that could be used in potential signaling and nutrient exchange between the bacteria and cyanobacteria such as Microcystis. Gene expression signatures of algal-derived carbon utilization for two isolates were identified in Microcystis blooms in Lake Erie and Lake Tai (Taihu) at low levels, suggesting these organisms are active and may have a functional role during Microcystis blooms in aggregates, but were largely missing from whole water samples. These findings build on the growing evidence that the bacterial microbiome associated with bloom-forming algae have the functional potential to contribute to nutrient exchange within bloom communities and interact with important bloom formers like Microcystis.Alexa K HokeGuadalupe ReynosoMorgan R SmithMalia I GardnerDominique J LockwoodNaomi E GilbertSteven W WilhelmIsabella R BeckerGrant J BrennanKatherine E CriderShannon R FarnanVictoria MendozaAlison C PooleZachary P ZimmermanLucy K UtzLouie L WurchMorgan M SteffenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257017 (2021) |
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Medicine R Science Q Alexa K Hoke Guadalupe Reynoso Morgan R Smith Malia I Gardner Dominique J Lockwood Naomi E Gilbert Steven W Wilhelm Isabella R Becker Grant J Brennan Katherine E Crider Shannon R Farnan Victoria Mendoza Alison C Poole Zachary P Zimmerman Lucy K Utz Louie L Wurch Morgan M Steffen Genomic signatures of Lake Erie bacteria suggest interaction in the Microcystis phycosphere. |
| description |
Microbial interactions in harmful algal bloom (HAB) communities have been examined in marine systems, but are poorly studied in fresh waters. To investigate HAB-microbe interactions, we isolated bacteria with close associations to bloom-forming cyanobacteria, Microcystis spp., during a 2017 bloom in the western basin of Lake Erie. The genomes of five isolates (Exiguobacterium sp. JMULE1, Enterobacter sp. JMULE2, Deinococcus sp. JMULE3, Paenibacillus sp. JMULE4, and Acidovorax sp. JMULE5.) were sequenced on a PacBio Sequel system. These genomes ranged in size from 3.1 Mbp (Exiguobacterium sp. JMULE1) to 5.7 Mbp (Enterobacter sp. JMULE2). The genomes were analyzed for genes relating to critical metabolic functions, including nitrogen reduction and carbon utilization. All five of the sequenced genomes contained genes that could be used in potential signaling and nutrient exchange between the bacteria and cyanobacteria such as Microcystis. Gene expression signatures of algal-derived carbon utilization for two isolates were identified in Microcystis blooms in Lake Erie and Lake Tai (Taihu) at low levels, suggesting these organisms are active and may have a functional role during Microcystis blooms in aggregates, but were largely missing from whole water samples. These findings build on the growing evidence that the bacterial microbiome associated with bloom-forming algae have the functional potential to contribute to nutrient exchange within bloom communities and interact with important bloom formers like Microcystis. |
| format |
article |
| author |
Alexa K Hoke Guadalupe Reynoso Morgan R Smith Malia I Gardner Dominique J Lockwood Naomi E Gilbert Steven W Wilhelm Isabella R Becker Grant J Brennan Katherine E Crider Shannon R Farnan Victoria Mendoza Alison C Poole Zachary P Zimmerman Lucy K Utz Louie L Wurch Morgan M Steffen |
| author_facet |
Alexa K Hoke Guadalupe Reynoso Morgan R Smith Malia I Gardner Dominique J Lockwood Naomi E Gilbert Steven W Wilhelm Isabella R Becker Grant J Brennan Katherine E Crider Shannon R Farnan Victoria Mendoza Alison C Poole Zachary P Zimmerman Lucy K Utz Louie L Wurch Morgan M Steffen |
| author_sort |
Alexa K Hoke |
| title |
Genomic signatures of Lake Erie bacteria suggest interaction in the Microcystis phycosphere. |
| title_short |
Genomic signatures of Lake Erie bacteria suggest interaction in the Microcystis phycosphere. |
| title_full |
Genomic signatures of Lake Erie bacteria suggest interaction in the Microcystis phycosphere. |
| title_fullStr |
Genomic signatures of Lake Erie bacteria suggest interaction in the Microcystis phycosphere. |
| title_full_unstemmed |
Genomic signatures of Lake Erie bacteria suggest interaction in the Microcystis phycosphere. |
| title_sort |
genomic signatures of lake erie bacteria suggest interaction in the microcystis phycosphere. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/cb519f536fe34085a0bc637a3ae83429 |
| work_keys_str_mv |
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