Characterization of <i>Haemophilus influenzae</i> Strains with Non-Enzymatic Resistance to β-Lactam Antibiotics Caused by Mutations in the PBP3 Gene in the Czech Republic in 2010–2018

The surveillance data on antibiotic resistance of <i>Haemophilus influenzae</i> have shown that strains with non-enzymatic resistance to β-lactam antibiotics have been on the rise in the Czech Republic over the last decade. This type of resistance is more difficult to detect than β-lacta...

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Autores principales:	Vladislav Jakubu, Lucia Malisova, Martin Musilek, Katarina Pomorska, Helena Zemlickova
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Haemophilus influenzae</i> <i>ftsI</i> β-lactam antibiotics non-enzymatic resistance Science Q
Acceso en línea:	https://doaj.org/article/cb534d6b0dda43168555d4d387c23d54
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spelling	oai:doaj.org-article:cb534d6b0dda43168555d4d387c23d542021-11-25T18:11:40ZCharacterization of <i>Haemophilus influenzae</i> Strains with Non-Enzymatic Resistance to β-Lactam Antibiotics Caused by Mutations in the PBP3 Gene in the Czech Republic in 2010–201810.3390/life111112602075-1729https://doaj.org/article/cb534d6b0dda43168555d4d387c23d542021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1260https://doaj.org/toc/2075-1729The surveillance data on antibiotic resistance of <i>Haemophilus influenzae</i> have shown that strains with non-enzymatic resistance to β-lactam antibiotics have been on the rise in the Czech Republic over the last decade. This type of resistance is more difficult to detect than β-lactamase production. Analysis of 228 <i>H. influenzae</i> strains revealed that isolates with non-enzymatic resistance to β-lactams due to mutations in the <i>ftsI</i> gene could be reliably demonstrated by single run testing of susceptibility to amoxicillin/clavulanic acid (sensitivity of detection is 84.6%), cefuroxime (92.6%), ampicillin and penicillin (both 95.7%). Thirty-seven different amino acid substitution combinations were detected in the PBP3 protein at 23 positions (V329I, D350N, S357N, A368T, M377I, S385T, A388V, L389F, P393L, A437S, I449V, G490E, I491V, R501L, A502S, A502T, A502V, V511A, R517H, I519L, N526K, A530S, and T532S). The most common combination (35%) of amino acid substitutions was the combination D350N, M377I, A502V, N526K. Epidemiological typing does not indicate a clonal spread of a particular MLST type. Altogether there has been detected 74 STs. The most prevalent ST 1034 was associated mainly with a combination D350N, M377I, A502V, N526K. Clonal analysis revealed six clonal complexes (CCs) with the founder found, eight CCs without founder and 33 singletons.Vladislav JakubuLucia MalisovaMartin MusilekKatarina PomorskaHelena ZemlickovaMDPI AGarticle<i>Haemophilus influenzae</i><i>ftsI</i>β-lactam antibioticsnon-enzymatic resistanceScienceQENLife, Vol 11, Iss 1260, p 1260 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Haemophilus influenzae</i> <i>ftsI</i> β-lactam antibiotics non-enzymatic resistance Science Q
spellingShingle	<i>Haemophilus influenzae</i> <i>ftsI</i> β-lactam antibiotics non-enzymatic resistance Science Q Vladislav Jakubu Lucia Malisova Martin Musilek Katarina Pomorska Helena Zemlickova Characterization of <i>Haemophilus influenzae</i> Strains with Non-Enzymatic Resistance to β-Lactam Antibiotics Caused by Mutations in the PBP3 Gene in the Czech Republic in 2010–2018
description	The surveillance data on antibiotic resistance of <i>Haemophilus influenzae</i> have shown that strains with non-enzymatic resistance to β-lactam antibiotics have been on the rise in the Czech Republic over the last decade. This type of resistance is more difficult to detect than β-lactamase production. Analysis of 228 <i>H. influenzae</i> strains revealed that isolates with non-enzymatic resistance to β-lactams due to mutations in the <i>ftsI</i> gene could be reliably demonstrated by single run testing of susceptibility to amoxicillin/clavulanic acid (sensitivity of detection is 84.6%), cefuroxime (92.6%), ampicillin and penicillin (both 95.7%). Thirty-seven different amino acid substitution combinations were detected in the PBP3 protein at 23 positions (V329I, D350N, S357N, A368T, M377I, S385T, A388V, L389F, P393L, A437S, I449V, G490E, I491V, R501L, A502S, A502T, A502V, V511A, R517H, I519L, N526K, A530S, and T532S). The most common combination (35%) of amino acid substitutions was the combination D350N, M377I, A502V, N526K. Epidemiological typing does not indicate a clonal spread of a particular MLST type. Altogether there has been detected 74 STs. The most prevalent ST 1034 was associated mainly with a combination D350N, M377I, A502V, N526K. Clonal analysis revealed six clonal complexes (CCs) with the founder found, eight CCs without founder and 33 singletons.
format	article
author	Vladislav Jakubu Lucia Malisova Martin Musilek Katarina Pomorska Helena Zemlickova
author_facet	Vladislav Jakubu Lucia Malisova Martin Musilek Katarina Pomorska Helena Zemlickova
author_sort	Vladislav Jakubu
title	Characterization of <i>Haemophilus influenzae</i> Strains with Non-Enzymatic Resistance to β-Lactam Antibiotics Caused by Mutations in the PBP3 Gene in the Czech Republic in 2010–2018
title_short	Characterization of <i>Haemophilus influenzae</i> Strains with Non-Enzymatic Resistance to β-Lactam Antibiotics Caused by Mutations in the PBP3 Gene in the Czech Republic in 2010–2018
title_full	Characterization of <i>Haemophilus influenzae</i> Strains with Non-Enzymatic Resistance to β-Lactam Antibiotics Caused by Mutations in the PBP3 Gene in the Czech Republic in 2010–2018
title_fullStr	Characterization of <i>Haemophilus influenzae</i> Strains with Non-Enzymatic Resistance to β-Lactam Antibiotics Caused by Mutations in the PBP3 Gene in the Czech Republic in 2010–2018
title_full_unstemmed	Characterization of <i>Haemophilus influenzae</i> Strains with Non-Enzymatic Resistance to β-Lactam Antibiotics Caused by Mutations in the PBP3 Gene in the Czech Republic in 2010–2018
title_sort	characterization of <i>haemophilus influenzae</i> strains with non-enzymatic resistance to β-lactam antibiotics caused by mutations in the pbp3 gene in the czech republic in 2010–2018
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cb534d6b0dda43168555d4d387c23d54
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Characterization of <i>Haemophilus influenzae</i> Strains with Non-Enzymatic Resistance to β-Lactam Antibiotics Caused by Mutations in the PBP3 Gene in the Czech Republic in 2010–2018

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