Expression and functional analysis of the lysine decarboxylase and copper amine oxidase genes from the endophytic fungus Colletotrichum gloeosporioides ES026

Abstract Huperzine A (HupA) isolated from Huperzia serrata is an important compound used to treat Alzheimer’s disease (AD). Recently, HupA was reported in various endophytic fungi, with Colletotrichum gloeosporioides ES026 previously isolated from H. serrata shown to produce HupA. In this study, we...

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Autores principales:	Xiangmei Zhang, Zhangqian Wang, Saad Jan, Qian Yang, Mo Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb553626c0a74524869f3e6e38e06ce5
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spelling	oai:doaj.org-article:cb553626c0a74524869f3e6e38e06ce52021-12-02T11:40:13ZExpression and functional analysis of the lysine decarboxylase and copper amine oxidase genes from the endophytic fungus Colletotrichum gloeosporioides ES02610.1038/s41598-017-02834-62045-2322https://doaj.org/article/cb553626c0a74524869f3e6e38e06ce52017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02834-6https://doaj.org/toc/2045-2322Abstract Huperzine A (HupA) isolated from Huperzia serrata is an important compound used to treat Alzheimer’s disease (AD). Recently, HupA was reported in various endophytic fungi, with Colletotrichum gloeosporioides ES026 previously isolated from H. serrata shown to produce HupA. In this study, we performed next-generation sequencing and de novo RNA sequencing of C. gloeosporioides ES026 to elucidate the molecular functions, biological processes, and biochemical pathways of these unique sequences. Gene ontology and Kyoto Encyclopedia of Genes and Genomes assignments allowed annotation of lysine decarboxylase (LDC) and copper amine oxidase (CAO) for their conversion of L-lysine to 5-aminopentanal during HupA biosynthesis. Additionally, we constructed a stable, high-yielding HupA-expression system resulting from the overexpression of CgLDC and CgCAO from the HupA-producing endophytic fungus C. gloeosporioides ES026 in Escherichia coli. Quantitative reverse transcription polymerase chain reaction analysis confirmed CgLDC and CgCAO expression, and quantitative determination of HupA levels was assessed by liquid chromatography high-resolution mass spectrometry, which revealed that elevated expression of CgLDC and CgCAO produced higher yields of HupA than those derived from C. gloeosporioides ES026. These results revealed CgLDC and CgCAO involvement in HupA biosynthesis and their key role in regulating HupA content in C. gloeosporioides ES026.Xiangmei ZhangZhangqian WangSaad JanQian YangMo WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiangmei Zhang Zhangqian Wang Saad Jan Qian Yang Mo Wang Expression and functional analysis of the lysine decarboxylase and copper amine oxidase genes from the endophytic fungus Colletotrichum gloeosporioides ES026
description	Abstract Huperzine A (HupA) isolated from Huperzia serrata is an important compound used to treat Alzheimer’s disease (AD). Recently, HupA was reported in various endophytic fungi, with Colletotrichum gloeosporioides ES026 previously isolated from H. serrata shown to produce HupA. In this study, we performed next-generation sequencing and de novo RNA sequencing of C. gloeosporioides ES026 to elucidate the molecular functions, biological processes, and biochemical pathways of these unique sequences. Gene ontology and Kyoto Encyclopedia of Genes and Genomes assignments allowed annotation of lysine decarboxylase (LDC) and copper amine oxidase (CAO) for their conversion of L-lysine to 5-aminopentanal during HupA biosynthesis. Additionally, we constructed a stable, high-yielding HupA-expression system resulting from the overexpression of CgLDC and CgCAO from the HupA-producing endophytic fungus C. gloeosporioides ES026 in Escherichia coli. Quantitative reverse transcription polymerase chain reaction analysis confirmed CgLDC and CgCAO expression, and quantitative determination of HupA levels was assessed by liquid chromatography high-resolution mass spectrometry, which revealed that elevated expression of CgLDC and CgCAO produced higher yields of HupA than those derived from C. gloeosporioides ES026. These results revealed CgLDC and CgCAO involvement in HupA biosynthesis and their key role in regulating HupA content in C. gloeosporioides ES026.
format	article
author	Xiangmei Zhang Zhangqian Wang Saad Jan Qian Yang Mo Wang
author_facet	Xiangmei Zhang Zhangqian Wang Saad Jan Qian Yang Mo Wang
author_sort	Xiangmei Zhang
title	Expression and functional analysis of the lysine decarboxylase and copper amine oxidase genes from the endophytic fungus Colletotrichum gloeosporioides ES026
title_short	Expression and functional analysis of the lysine decarboxylase and copper amine oxidase genes from the endophytic fungus Colletotrichum gloeosporioides ES026
title_full	Expression and functional analysis of the lysine decarboxylase and copper amine oxidase genes from the endophytic fungus Colletotrichum gloeosporioides ES026
title_fullStr	Expression and functional analysis of the lysine decarboxylase and copper amine oxidase genes from the endophytic fungus Colletotrichum gloeosporioides ES026
title_full_unstemmed	Expression and functional analysis of the lysine decarboxylase and copper amine oxidase genes from the endophytic fungus Colletotrichum gloeosporioides ES026
title_sort	expression and functional analysis of the lysine decarboxylase and copper amine oxidase genes from the endophytic fungus colletotrichum gloeosporioides es026
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cb553626c0a74524869f3e6e38e06ce5
work_keys_str_mv	AT xiangmeizhang expressionandfunctionalanalysisofthelysinedecarboxylaseandcopperamineoxidasegenesfromtheendophyticfunguscolletotrichumgloeosporioideses026 AT zhangqianwang expressionandfunctionalanalysisofthelysinedecarboxylaseandcopperamineoxidasegenesfromtheendophyticfunguscolletotrichumgloeosporioideses026 AT saadjan expressionandfunctionalanalysisofthelysinedecarboxylaseandcopperamineoxidasegenesfromtheendophyticfunguscolletotrichumgloeosporioideses026 AT qianyang expressionandfunctionalanalysisofthelysinedecarboxylaseandcopperamineoxidasegenesfromtheendophyticfunguscolletotrichumgloeosporioideses026 AT mowang expressionandfunctionalanalysisofthelysinedecarboxylaseandcopperamineoxidasegenesfromtheendophyticfunguscolletotrichumgloeosporioideses026
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Expression and functional analysis of the lysine decarboxylase and copper amine oxidase genes from the endophytic fungus Colletotrichum gloeosporioides ES026

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