Evaluation of the possible transmission of BSE and scrapie to gilthead sea bream (Sparus aurata).

In transmissible spongiform encephalopathies (TSEs), a group of fatal neurodegenerative disorders affecting many species, the key event in disease pathogenesis is the accumulation of an abnormal conformational isoform (PrP(Sc)) of the host-encoded cellular prion protein (PrP(C)). While the precise m...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Evgenia Salta, Cynthia Panagiotidis, Konstantinos Teliousis, Spyros Petrakis, Eleftherios Eleftheriadis, Fotis Arapoglou, Nikolaos Grigoriadis, Anna Nicolaou, Eleni Kaldrymidou, Grigorios Krey, Theodoros Sklaviadis
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb58a0df31424a588557db46f17f7820
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cb58a0df31424a588557db46f17f7820
record_format	dspace
spelling	oai:doaj.org-article:cb58a0df31424a588557db46f17f78202021-11-25T06:21:24ZEvaluation of the possible transmission of BSE and scrapie to gilthead sea bream (Sparus aurata).1932-620310.1371/journal.pone.0006175https://doaj.org/article/cb58a0df31424a588557db46f17f78202009-07-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19636413/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203In transmissible spongiform encephalopathies (TSEs), a group of fatal neurodegenerative disorders affecting many species, the key event in disease pathogenesis is the accumulation of an abnormal conformational isoform (PrP(Sc)) of the host-encoded cellular prion protein (PrP(C)). While the precise mechanism of the PrP(C) to PrP(Sc) conversion is not understood, it is clear that host PrP(C) expression is a prerequisite for effective infectious prion propagation. Although there have been many studies on TSEs in mammalian species, little is known about TSE pathogenesis in fish. Here we show that while gilthead sea bream (Sparus aurata) orally challenged with brain homogenates prepared either from a BSE infected cow or from scrapie infected sheep developed no clinical prion disease, the brains of TSE-fed fish sampled two years after challenge did show signs of neurodegeneration and accumulation of deposits that reacted positively with antibodies raised against sea bream PrP. The control groups, fed with brains from uninfected animals, showed no such signs. Remarkably, the deposits developed much more rapidly and extensively in fish inoculated with BSE-infected material than in the ones challenged with the scrapie-infected brain homogenate, with numerous deposits being proteinase K-resistant. These plaque-like aggregates exhibited congophilia and birefringence in polarized light, consistent with an amyloid-like component. The neurodegeneration and abnormal deposition in the brains of fish challenged with prion, especially BSE, raises concerns about the potential risk to public health. As fish aquaculture is an economically important industry providing high protein nutrition for humans and other mammalian species, the prospect of farmed fish being contaminated with infectious mammalian PrP(Sc), or of a prion disease developing in farmed fish is alarming and requires further evaluation.Evgenia SaltaCynthia PanagiotidisKonstantinos TeliousisSpyros PetrakisEleftherios EleftheriadisFotis ArapoglouNikolaos GrigoriadisAnna NicolaouEleni KaldrymidouGrigorios KreyTheodoros SklaviadisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 7, p e6175 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Evgenia Salta Cynthia Panagiotidis Konstantinos Teliousis Spyros Petrakis Eleftherios Eleftheriadis Fotis Arapoglou Nikolaos Grigoriadis Anna Nicolaou Eleni Kaldrymidou Grigorios Krey Theodoros Sklaviadis Evaluation of the possible transmission of BSE and scrapie to gilthead sea bream (Sparus aurata).
description	In transmissible spongiform encephalopathies (TSEs), a group of fatal neurodegenerative disorders affecting many species, the key event in disease pathogenesis is the accumulation of an abnormal conformational isoform (PrP(Sc)) of the host-encoded cellular prion protein (PrP(C)). While the precise mechanism of the PrP(C) to PrP(Sc) conversion is not understood, it is clear that host PrP(C) expression is a prerequisite for effective infectious prion propagation. Although there have been many studies on TSEs in mammalian species, little is known about TSE pathogenesis in fish. Here we show that while gilthead sea bream (Sparus aurata) orally challenged with brain homogenates prepared either from a BSE infected cow or from scrapie infected sheep developed no clinical prion disease, the brains of TSE-fed fish sampled two years after challenge did show signs of neurodegeneration and accumulation of deposits that reacted positively with antibodies raised against sea bream PrP. The control groups, fed with brains from uninfected animals, showed no such signs. Remarkably, the deposits developed much more rapidly and extensively in fish inoculated with BSE-infected material than in the ones challenged with the scrapie-infected brain homogenate, with numerous deposits being proteinase K-resistant. These plaque-like aggregates exhibited congophilia and birefringence in polarized light, consistent with an amyloid-like component. The neurodegeneration and abnormal deposition in the brains of fish challenged with prion, especially BSE, raises concerns about the potential risk to public health. As fish aquaculture is an economically important industry providing high protein nutrition for humans and other mammalian species, the prospect of farmed fish being contaminated with infectious mammalian PrP(Sc), or of a prion disease developing in farmed fish is alarming and requires further evaluation.
format	article
author	Evgenia Salta Cynthia Panagiotidis Konstantinos Teliousis Spyros Petrakis Eleftherios Eleftheriadis Fotis Arapoglou Nikolaos Grigoriadis Anna Nicolaou Eleni Kaldrymidou Grigorios Krey Theodoros Sklaviadis
author_facet	Evgenia Salta Cynthia Panagiotidis Konstantinos Teliousis Spyros Petrakis Eleftherios Eleftheriadis Fotis Arapoglou Nikolaos Grigoriadis Anna Nicolaou Eleni Kaldrymidou Grigorios Krey Theodoros Sklaviadis
author_sort	Evgenia Salta
title	Evaluation of the possible transmission of BSE and scrapie to gilthead sea bream (Sparus aurata).
title_short	Evaluation of the possible transmission of BSE and scrapie to gilthead sea bream (Sparus aurata).
title_full	Evaluation of the possible transmission of BSE and scrapie to gilthead sea bream (Sparus aurata).
title_fullStr	Evaluation of the possible transmission of BSE and scrapie to gilthead sea bream (Sparus aurata).
title_full_unstemmed	Evaluation of the possible transmission of BSE and scrapie to gilthead sea bream (Sparus aurata).
title_sort	evaluation of the possible transmission of bse and scrapie to gilthead sea bream (sparus aurata).
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/cb58a0df31424a588557db46f17f7820
work_keys_str_mv	AT evgeniasalta evaluationofthepossibletransmissionofbseandscrapietogiltheadseabreamsparusaurata AT cynthiapanagiotidis evaluationofthepossibletransmissionofbseandscrapietogiltheadseabreamsparusaurata AT konstantinosteliousis evaluationofthepossibletransmissionofbseandscrapietogiltheadseabreamsparusaurata AT spyrospetrakis evaluationofthepossibletransmissionofbseandscrapietogiltheadseabreamsparusaurata AT eleftherioseleftheriadis evaluationofthepossibletransmissionofbseandscrapietogiltheadseabreamsparusaurata AT fotisarapoglou evaluationofthepossibletransmissionofbseandscrapietogiltheadseabreamsparusaurata AT nikolaosgrigoriadis evaluationofthepossibletransmissionofbseandscrapietogiltheadseabreamsparusaurata AT annanicolaou evaluationofthepossibletransmissionofbseandscrapietogiltheadseabreamsparusaurata AT elenikaldrymidou evaluationofthepossibletransmissionofbseandscrapietogiltheadseabreamsparusaurata AT grigorioskrey evaluationofthepossibletransmissionofbseandscrapietogiltheadseabreamsparusaurata AT theodorossklaviadis evaluationofthepossibletransmissionofbseandscrapietogiltheadseabreamsparusaurata
_version_	1718413827503030272

Evaluation of the possible transmission of BSE and scrapie to gilthead sea bream (Sparus aurata).

Ejemplares similares