Tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in Arabidopsis

Abstract A great proportion of nitrate taken up by plants is stored in vacuoles. Vacuolar nitrate accumulation and release is of great importance to nitrate reallocation and efficient utilization. However, how plants mediate nitrate efflux from vacuoles to cytoplasm is largely unknown. The current s...

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Autores principales:	Ya-Ni He, Jia-Shi Peng, Yao Cai, De-Fen Liu, Yuan Guan, Hong-Ying Yi, Ji-Ming Gong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb5928e5155b40919f2f6e8b4badcc67
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spelling	oai:doaj.org-article:cb5928e5155b40919f2f6e8b4badcc672021-12-02T12:32:53ZTonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in Arabidopsis10.1038/s41598-017-06744-52045-2322https://doaj.org/article/cb5928e5155b40919f2f6e8b4badcc672017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06744-5https://doaj.org/toc/2045-2322Abstract A great proportion of nitrate taken up by plants is stored in vacuoles. Vacuolar nitrate accumulation and release is of great importance to nitrate reallocation and efficient utilization. However, how plants mediate nitrate efflux from vacuoles to cytoplasm is largely unknown. The current study identified NPF5.11, NPF5.12 and NPF5.16 as vacuolar nitrate efflux transporters in Arabidopsis. Histochemical analysis showed that NPF5.11, NPF5.12 and NPF5.16 were expressed preferentially in root pericycle cells and xylem parenchyma cells, and further analysis showed that these proteins were tonoplast-localized. Functional characterization using cRNA-injected Xenopus laevis oocytes showed that NPF5.11, NPF5.12 and NPF5.16 were low-affinity, pH-dependent nitrate uptake transporters. In npf5.11 npf5.12 npf5.16 triple mutant lines, more root-fed 15NO3 − was translocated to shoots compared to the wild type control. In the NPF5.12 overexpression lines, proportionally less nitrate was maintained in roots. These data together suggested that NPF5.11, NPF5.12 and NPF5.16 might function to uptake nitrate from vacuoles into cytosol, thus serving as important players to modulate nitrate allocation between roots and shoots.Ya-Ni HeJia-Shi PengYao CaiDe-Fen LiuYuan GuanHong-Ying YiJi-Ming GongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ya-Ni He Jia-Shi Peng Yao Cai De-Fen Liu Yuan Guan Hong-Ying Yi Ji-Ming Gong Tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in Arabidopsis
description	Abstract A great proportion of nitrate taken up by plants is stored in vacuoles. Vacuolar nitrate accumulation and release is of great importance to nitrate reallocation and efficient utilization. However, how plants mediate nitrate efflux from vacuoles to cytoplasm is largely unknown. The current study identified NPF5.11, NPF5.12 and NPF5.16 as vacuolar nitrate efflux transporters in Arabidopsis. Histochemical analysis showed that NPF5.11, NPF5.12 and NPF5.16 were expressed preferentially in root pericycle cells and xylem parenchyma cells, and further analysis showed that these proteins were tonoplast-localized. Functional characterization using cRNA-injected Xenopus laevis oocytes showed that NPF5.11, NPF5.12 and NPF5.16 were low-affinity, pH-dependent nitrate uptake transporters. In npf5.11 npf5.12 npf5.16 triple mutant lines, more root-fed 15NO3 − was translocated to shoots compared to the wild type control. In the NPF5.12 overexpression lines, proportionally less nitrate was maintained in roots. These data together suggested that NPF5.11, NPF5.12 and NPF5.16 might function to uptake nitrate from vacuoles into cytosol, thus serving as important players to modulate nitrate allocation between roots and shoots.
format	article
author	Ya-Ni He Jia-Shi Peng Yao Cai De-Fen Liu Yuan Guan Hong-Ying Yi Ji-Ming Gong
author_facet	Ya-Ni He Jia-Shi Peng Yao Cai De-Fen Liu Yuan Guan Hong-Ying Yi Ji-Ming Gong
author_sort	Ya-Ni He
title	Tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in Arabidopsis
title_short	Tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in Arabidopsis
title_full	Tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in Arabidopsis
title_fullStr	Tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in Arabidopsis
title_full_unstemmed	Tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in Arabidopsis
title_sort	tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in arabidopsis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cb5928e5155b40919f2f6e8b4badcc67
work_keys_str_mv	AT yanihe tonoplastlocalizednitrateuptaketransportersinvolvedinvacuolarnitrateeffluxandreallocationinarabidopsis AT jiashipeng tonoplastlocalizednitrateuptaketransportersinvolvedinvacuolarnitrateeffluxandreallocationinarabidopsis AT yaocai tonoplastlocalizednitrateuptaketransportersinvolvedinvacuolarnitrateeffluxandreallocationinarabidopsis AT defenliu tonoplastlocalizednitrateuptaketransportersinvolvedinvacuolarnitrateeffluxandreallocationinarabidopsis AT yuanguan tonoplastlocalizednitrateuptaketransportersinvolvedinvacuolarnitrateeffluxandreallocationinarabidopsis AT hongyingyi tonoplastlocalizednitrateuptaketransportersinvolvedinvacuolarnitrateeffluxandreallocationinarabidopsis AT jiminggong tonoplastlocalizednitrateuptaketransportersinvolvedinvacuolarnitrateeffluxandreallocationinarabidopsis
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Tonoplast-localized nitrate uptake transporters involved in vacuolar nitrate efflux and reallocation in Arabidopsis

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