Murine melanoma-infiltrating dendritic cells are defective in antigen presenting function regardless of the presence of CD4CD25 regulatory T cells.

Tumor-infiltrating dendritic cells are often ineffective at presenting tumor-derived antigen in vivo, a defect usually ascribed to the suppressive tumor environment. We investigated the effects of depleting CD4(+)CD25(+) "natural" regulatory T cells (Treg) on the frequency, phenotype and f...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Haley Ataera, Evelyn Hyde, Kylie M Price, Patrizia Stoitzner, Franca Ronchese
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb6507b2d4f64d1fa837997dc83498c3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cb6507b2d4f64d1fa837997dc83498c3
record_format	dspace
spelling	oai:doaj.org-article:cb6507b2d4f64d1fa837997dc83498c32021-11-18T06:57:50ZMurine melanoma-infiltrating dendritic cells are defective in antigen presenting function regardless of the presence of CD4CD25 regulatory T cells.1932-620310.1371/journal.pone.0017515https://doaj.org/article/cb6507b2d4f64d1fa837997dc83498c32011-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21390236/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Tumor-infiltrating dendritic cells are often ineffective at presenting tumor-derived antigen in vivo, a defect usually ascribed to the suppressive tumor environment. We investigated the effects of depleting CD4(+)CD25(+) "natural" regulatory T cells (Treg) on the frequency, phenotype and function of total dendritic cell populations in B16.OVA tumors and in tumor-draining lymph nodes. Intraperitoneal injection of the anti-CD25 monoclonal antibody PC61 reduced Treg frequency in blood and tumors, but did not affect the frequency of tumor-infiltrating dendritic cells, or their expression of CD40, CD86 and MHCII. Tumor-infiltrating dendritic cells from PC61-treated or untreated mice induced the proliferation of allogeneic T cells in vitro, but could not induce proliferation of OVA-specific OTI and OTII T cells unless specific peptide antigen was added in culture. Some proliferation of naïve, OVA-specific OTI T cells, but not OTII T cells, was observed in the tumor-draining LN of mice carrying B16.OVA tumors, however, this was not improved by PC61 treatment. Experiments using RAG1(-/-) hosts adoptively transferred with OTI and CD25-depleted OTII cells also failed to show improved OTI and OTII T cell proliferation in vivo compared to C57BL/6 hosts. We conclude that the defective presentation of B16.OVA tumor antigen by tumor-infiltrating dendritic cells and in the tumor-draining lymph node is not due to the presence of "natural" CD4(+)CD25(+) Treg.Haley AtaeraEvelyn HydeKylie M PricePatrizia StoitznerFranca RonchesePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 3, p e17515 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Haley Ataera Evelyn Hyde Kylie M Price Patrizia Stoitzner Franca Ronchese Murine melanoma-infiltrating dendritic cells are defective in antigen presenting function regardless of the presence of CD4CD25 regulatory T cells.
description	Tumor-infiltrating dendritic cells are often ineffective at presenting tumor-derived antigen in vivo, a defect usually ascribed to the suppressive tumor environment. We investigated the effects of depleting CD4(+)CD25(+) "natural" regulatory T cells (Treg) on the frequency, phenotype and function of total dendritic cell populations in B16.OVA tumors and in tumor-draining lymph nodes. Intraperitoneal injection of the anti-CD25 monoclonal antibody PC61 reduced Treg frequency in blood and tumors, but did not affect the frequency of tumor-infiltrating dendritic cells, or their expression of CD40, CD86 and MHCII. Tumor-infiltrating dendritic cells from PC61-treated or untreated mice induced the proliferation of allogeneic T cells in vitro, but could not induce proliferation of OVA-specific OTI and OTII T cells unless specific peptide antigen was added in culture. Some proliferation of naïve, OVA-specific OTI T cells, but not OTII T cells, was observed in the tumor-draining LN of mice carrying B16.OVA tumors, however, this was not improved by PC61 treatment. Experiments using RAG1(-/-) hosts adoptively transferred with OTI and CD25-depleted OTII cells also failed to show improved OTI and OTII T cell proliferation in vivo compared to C57BL/6 hosts. We conclude that the defective presentation of B16.OVA tumor antigen by tumor-infiltrating dendritic cells and in the tumor-draining lymph node is not due to the presence of "natural" CD4(+)CD25(+) Treg.
format	article
author	Haley Ataera Evelyn Hyde Kylie M Price Patrizia Stoitzner Franca Ronchese
author_facet	Haley Ataera Evelyn Hyde Kylie M Price Patrizia Stoitzner Franca Ronchese
author_sort	Haley Ataera
title	Murine melanoma-infiltrating dendritic cells are defective in antigen presenting function regardless of the presence of CD4CD25 regulatory T cells.
title_short	Murine melanoma-infiltrating dendritic cells are defective in antigen presenting function regardless of the presence of CD4CD25 regulatory T cells.
title_full	Murine melanoma-infiltrating dendritic cells are defective in antigen presenting function regardless of the presence of CD4CD25 regulatory T cells.
title_fullStr	Murine melanoma-infiltrating dendritic cells are defective in antigen presenting function regardless of the presence of CD4CD25 regulatory T cells.
title_full_unstemmed	Murine melanoma-infiltrating dendritic cells are defective in antigen presenting function regardless of the presence of CD4CD25 regulatory T cells.
title_sort	murine melanoma-infiltrating dendritic cells are defective in antigen presenting function regardless of the presence of cd4cd25 regulatory t cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/cb6507b2d4f64d1fa837997dc83498c3
work_keys_str_mv	AT haleyataera murinemelanomainfiltratingdendriticcellsaredefectiveinantigenpresentingfunctionregardlessofthepresenceofcd4cd25regulatorytcells AT evelynhyde murinemelanomainfiltratingdendriticcellsaredefectiveinantigenpresentingfunctionregardlessofthepresenceofcd4cd25regulatorytcells AT kyliemprice murinemelanomainfiltratingdendriticcellsaredefectiveinantigenpresentingfunctionregardlessofthepresenceofcd4cd25regulatorytcells AT patriziastoitzner murinemelanomainfiltratingdendriticcellsaredefectiveinantigenpresentingfunctionregardlessofthepresenceofcd4cd25regulatorytcells AT francaronchese murinemelanomainfiltratingdendriticcellsaredefectiveinantigenpresentingfunctionregardlessofthepresenceofcd4cd25regulatorytcells
_version_	1718424133131304960

Murine melanoma-infiltrating dendritic cells are defective in antigen presenting function regardless of the presence of CD4CD25 regulatory T cells.

Ejemplares similares