Association between calcifying nanoparticles and placental calcification

Yanan Guo1, Dechun Zhang1, He Lu1, Shuang Luo2, Xuecheng Shen31Molecular Medicine and Tumor Research Center, Chongqing Medical University, Yuzhong District, 2Department of Obstetrics and Gynecology, First Affiliated Hospital of Chongqing Medical University, 3Urological Research Institute of PLA, Sou...

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Autores principales:	Zhang D, Lu H, Luo S, Shen X, Guo Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/cb6a247fd30d422098a37c8b492d5f5a
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spelling	oai:doaj.org-article:cb6a247fd30d422098a37c8b492d5f5a2021-12-02T08:44:27ZAssociation between calcifying nanoparticles and placental calcification1176-91141178-2013https://doaj.org/article/cb6a247fd30d422098a37c8b492d5f5a2012-03-01T00:00:00Zhttp://www.dovepress.com/association-between-calcifying-nanoparticles-and-placental-calcificati-a9575https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yanan Guo1, Dechun Zhang1, He Lu1, Shuang Luo2, Xuecheng Shen31Molecular Medicine and Tumor Research Center, Chongqing Medical University, Yuzhong District, 2Department of Obstetrics and Gynecology, First Affiliated Hospital of Chongqing Medical University, 3Urological Research Institute of PLA, Southwest Hospital, Third Military Medical University, Chongqing, People&#39;s Republic of ChinaBackground: The purpose of this study was to examine the possible contribution of calcifying nanoparticles to the pathogenesis of placental calcification.Methods: Calcified placental tissues and distal tissue samples were collected from 36 confirmed placental calcification cases. In addition, 20 normal placental tissue samples were obtained as a control group. All the tissue samples were cultured using special nanobacterial culture methods. The cultured calcifying nanoparticles were examined by transmission electron microscopy (TEM), and their growth was monitored by optical density (OD) at a wavelength of 650 nm. 16S rRNA gene expression of the cultured calcifying nanoparticles was also isolated and sequenced.Results: Novel calcifying nanoparticles wrapped with electron-dense shells between 50 nm to 500 nm in diameter were observed in the extracellular matrix of calcified placental tissues. They were detected in placental villi and hydroxyapatite crystals, and contained &ldquo;nucleic acid-like materials&rdquo;. After isolation and four weeks of culture, 28 of 36 calcified placental tissue samples showed white granular precipitates attached to the bottom of the culture tubes. OD650 measurements indicated that the precipitates from the calcified placental tissues were able to grow in culture, whereas no such precipitates from the control tissues were observed. The 16S rRNA genes were isolated from the cultured calcifying nanoparticles and calcified placental tissues, and their gene sequencing results implied that calcifying nanoparticles were novel nanobacteria (GenBank JF823648).Conclusion: Our results suggest that these novel calcifying nanoparticles may play a role in placental calcification.Keywords: calcifying nanoparticles, nanobacteria, placental calcification, 16S rRNA geneZhang DLu HLuo SShen XGuo YDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 1679-1686 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zhang D Lu H Luo S Shen X Guo Y Association between calcifying nanoparticles and placental calcification
description	Yanan Guo1, Dechun Zhang1, He Lu1, Shuang Luo2, Xuecheng Shen31Molecular Medicine and Tumor Research Center, Chongqing Medical University, Yuzhong District, 2Department of Obstetrics and Gynecology, First Affiliated Hospital of Chongqing Medical University, 3Urological Research Institute of PLA, Southwest Hospital, Third Military Medical University, Chongqing, People&#39;s Republic of ChinaBackground: The purpose of this study was to examine the possible contribution of calcifying nanoparticles to the pathogenesis of placental calcification.Methods: Calcified placental tissues and distal tissue samples were collected from 36 confirmed placental calcification cases. In addition, 20 normal placental tissue samples were obtained as a control group. All the tissue samples were cultured using special nanobacterial culture methods. The cultured calcifying nanoparticles were examined by transmission electron microscopy (TEM), and their growth was monitored by optical density (OD) at a wavelength of 650 nm. 16S rRNA gene expression of the cultured calcifying nanoparticles was also isolated and sequenced.Results: Novel calcifying nanoparticles wrapped with electron-dense shells between 50 nm to 500 nm in diameter were observed in the extracellular matrix of calcified placental tissues. They were detected in placental villi and hydroxyapatite crystals, and contained &ldquo;nucleic acid-like materials&rdquo;. After isolation and four weeks of culture, 28 of 36 calcified placental tissue samples showed white granular precipitates attached to the bottom of the culture tubes. OD650 measurements indicated that the precipitates from the calcified placental tissues were able to grow in culture, whereas no such precipitates from the control tissues were observed. The 16S rRNA genes were isolated from the cultured calcifying nanoparticles and calcified placental tissues, and their gene sequencing results implied that calcifying nanoparticles were novel nanobacteria (GenBank JF823648).Conclusion: Our results suggest that these novel calcifying nanoparticles may play a role in placental calcification.Keywords: calcifying nanoparticles, nanobacteria, placental calcification, 16S rRNA gene
format	article
author	Zhang D Lu H Luo S Shen X Guo Y
author_facet	Zhang D Lu H Luo S Shen X Guo Y
author_sort	Zhang D
title	Association between calcifying nanoparticles and placental calcification
title_short	Association between calcifying nanoparticles and placental calcification
title_full	Association between calcifying nanoparticles and placental calcification
title_fullStr	Association between calcifying nanoparticles and placental calcification
title_full_unstemmed	Association between calcifying nanoparticles and placental calcification
title_sort	association between calcifying nanoparticles and placental calcification
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/cb6a247fd30d422098a37c8b492d5f5a
work_keys_str_mv	AT zhangd associationbetweencalcifyingnanoparticlesandplacentalcalcification AT luh associationbetweencalcifyingnanoparticlesandplacentalcalcification AT luos associationbetweencalcifyingnanoparticlesandplacentalcalcification AT shenx associationbetweencalcifyingnanoparticlesandplacentalcalcification AT guoy associationbetweencalcifyingnanoparticlesandplacentalcalcification
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Association between calcifying nanoparticles and placental calcification

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