Bacitracin-Ag Nanoclusters as a Novel Antibacterial Agent Combats <i>Shigella flexneri</i> by Disrupting Cell Membrane and Inhibiting Biofilm Formation

A novel nanomaterial Bacitracin-Ag Nanoclusters (Bacitracin-AgNCs) was formed to achieve a better antibacterial effect on <i>Shigella flexneri</i> which poses a serious threat to human health. In the current study, X-ray photoelectron spectrometer (XPS), Fourier transform infrared (FTIR)...

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Autores principales:	Lin Wang, Liu Liu, Xiaotong Zhou
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	Ag nano bacitracin <i>Shigella flexneri</i> inhibition biofilm Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/cb6ddeb7058540ea8afa8899674b0df7
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spelling	oai:doaj.org-article:cb6ddeb7058540ea8afa8899674b0df72021-11-25T18:30:55ZBacitracin-Ag Nanoclusters as a Novel Antibacterial Agent Combats <i>Shigella flexneri</i> by Disrupting Cell Membrane and Inhibiting Biofilm Formation10.3390/nano111129282079-4991https://doaj.org/article/cb6ddeb7058540ea8afa8899674b0df72021-11-01T00:00:00Zhttps://www.mdpi.com/2079-4991/11/11/2928https://doaj.org/toc/2079-4991A novel nanomaterial Bacitracin-Ag Nanoclusters (Bacitracin-AgNCs) was formed to achieve a better antibacterial effect on <i>Shigella flexneri</i> which poses a serious threat to human health. In the current study, X-ray photoelectron spectrometer (XPS), Fourier transform infrared (FTIR), field-emission scanning electron microscopy (FESEM), high resolution transmission electron microscopy (HR-TEM) and thermal gravimetric analysis (TGA) were used to characterize the properties of composited Bacitracin-AgNCs. Furthermore, the inhibitory effects of Bacitracin-AgNCs against <i>S. flexneri</i> were explored, and the inhibition mechanism was discussed in terms of its aspects of cell membrane ravage, ATPase activity decline and biofilm inhibition. The results reveal that the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Bacitracin-AgNCs against <i>S. flexneri</i> were 0.03 mg/mL and 4 mg/mL. Bacitracin-AgNCs may cause irreversible impairment to cells and greatly change the cell morphology. The cell membrane integrity of <i>S. flexneri</i> was destroyed with changes in the characteristics of membrane permeability and intracellular substances leakage. Moreover, our study further proved that Bacitracin-AgNCs significantly inhibited the formation of <i>S. flexneri</i> biofilms and reduced the number of viable bacteria in biofilm. These findings provide a potential method for the exploitation of organic composite nanomaterials as a novel antimicrobial agent and its application in the food industry.Lin WangLiu LiuXiaotong ZhouMDPI AGarticleAgnanobacitracin<i>Shigella flexneri</i>inhibitionbiofilmChemistryQD1-999ENNanomaterials, Vol 11, Iss 2928, p 2928 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ag nano bacitracin <i>Shigella flexneri</i> inhibition biofilm Chemistry QD1-999
spellingShingle	Ag nano bacitracin <i>Shigella flexneri</i> inhibition biofilm Chemistry QD1-999 Lin Wang Liu Liu Xiaotong Zhou Bacitracin-Ag Nanoclusters as a Novel Antibacterial Agent Combats <i>Shigella flexneri</i> by Disrupting Cell Membrane and Inhibiting Biofilm Formation
description	A novel nanomaterial Bacitracin-Ag Nanoclusters (Bacitracin-AgNCs) was formed to achieve a better antibacterial effect on <i>Shigella flexneri</i> which poses a serious threat to human health. In the current study, X-ray photoelectron spectrometer (XPS), Fourier transform infrared (FTIR), field-emission scanning electron microscopy (FESEM), high resolution transmission electron microscopy (HR-TEM) and thermal gravimetric analysis (TGA) were used to characterize the properties of composited Bacitracin-AgNCs. Furthermore, the inhibitory effects of Bacitracin-AgNCs against <i>S. flexneri</i> were explored, and the inhibition mechanism was discussed in terms of its aspects of cell membrane ravage, ATPase activity decline and biofilm inhibition. The results reveal that the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Bacitracin-AgNCs against <i>S. flexneri</i> were 0.03 mg/mL and 4 mg/mL. Bacitracin-AgNCs may cause irreversible impairment to cells and greatly change the cell morphology. The cell membrane integrity of <i>S. flexneri</i> was destroyed with changes in the characteristics of membrane permeability and intracellular substances leakage. Moreover, our study further proved that Bacitracin-AgNCs significantly inhibited the formation of <i>S. flexneri</i> biofilms and reduced the number of viable bacteria in biofilm. These findings provide a potential method for the exploitation of organic composite nanomaterials as a novel antimicrobial agent and its application in the food industry.
format	article
author	Lin Wang Liu Liu Xiaotong Zhou
author_facet	Lin Wang Liu Liu Xiaotong Zhou
author_sort	Lin Wang
title	Bacitracin-Ag Nanoclusters as a Novel Antibacterial Agent Combats <i>Shigella flexneri</i> by Disrupting Cell Membrane and Inhibiting Biofilm Formation
title_short	Bacitracin-Ag Nanoclusters as a Novel Antibacterial Agent Combats <i>Shigella flexneri</i> by Disrupting Cell Membrane and Inhibiting Biofilm Formation
title_full	Bacitracin-Ag Nanoclusters as a Novel Antibacterial Agent Combats <i>Shigella flexneri</i> by Disrupting Cell Membrane and Inhibiting Biofilm Formation
title_fullStr	Bacitracin-Ag Nanoclusters as a Novel Antibacterial Agent Combats <i>Shigella flexneri</i> by Disrupting Cell Membrane and Inhibiting Biofilm Formation
title_full_unstemmed	Bacitracin-Ag Nanoclusters as a Novel Antibacterial Agent Combats <i>Shigella flexneri</i> by Disrupting Cell Membrane and Inhibiting Biofilm Formation
title_sort	bacitracin-ag nanoclusters as a novel antibacterial agent combats <i>shigella flexneri</i> by disrupting cell membrane and inhibiting biofilm formation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cb6ddeb7058540ea8afa8899674b0df7
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Bacitracin-Ag Nanoclusters as a Novel Antibacterial Agent Combats <i>Shigella flexneri</i> by Disrupting Cell Membrane and Inhibiting Biofilm Formation

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