Bioconjugates of PAMAM dendrimers with trans-retinal, pyridoxal, and pyridoxal phosphate

A Filipowicz, S WołowiecDepartment of Cosmetology, University of Information Technology and Management in Rzesz&oacute;w, Rzesz&oacute;w, PolandBackground: Bioconjugates of a polyamidoamine (PAMAM) G3 dendrimer and an aldehyde were synthesized as carriers for vitamins A and B6, and t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Filipowicz A, Wołowiec S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/cb6fff3636924f199b788698ae53b7f7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cb6fff3636924f199b788698ae53b7f7
record_format	dspace
spelling	oai:doaj.org-article:cb6fff3636924f199b788698ae53b7f72021-12-02T08:44:27ZBioconjugates of PAMAM dendrimers with trans-retinal, pyridoxal, and pyridoxal phosphate1176-91141178-2013https://doaj.org/article/cb6fff3636924f199b788698ae53b7f72012-09-01T00:00:00Zhttp://www.dovepress.com/bioconjugates-of-pamam-dendrimers-with-trans-retinal-pyridoxal-and-pyr-a10915https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013A Filipowicz, S WołowiecDepartment of Cosmetology, University of Information Technology and Management in Rzesz&oacute;w, Rzesz&oacute;w, PolandBackground: Bioconjugates of a polyamidoamine (PAMAM) G3 dendrimer and an aldehyde were synthesized as carriers for vitamins A and B6, and the bioavailability of these vitamins for skin nutrition was investigated.Methods: Nuclear magnetic resonance (NMR) and ultraviolet-visible methods were used to characterize the structure of the bioconjugates and for monitoring release of pyridoxal (Pyr) and pyridoxal phosphate (PLP) from these bioconjugates in vitro. A skin model permeation of bioconjugates was also studied in a Franz chamber.Results: A transdermal G3 PAMAM dendrimer was used to synthesize bioconjugates with trans-retinal (Ret), pyridoxal (Pyr), or PLP. These nanomolecules, containing up to four covalently linked Ret, Pyr, or PLP (G34Ret, G34Pyr, and G34PLP), were able to permeate the skin, as demonstrated in vitro using a model skin membrane. PLP and Pyr bound to a macromolecular vehicle were active cofactors for glutamic pyruvic transaminase, as shown by 1H NMR spectral monitoring of the progress of the L-alanine + &alpha;-ketoglutarate &rarr; glutamic acid + pyruvic acid reaction.Conclusion: PAMAM-PLP, PAMAM-Pyr, and PAMAM-Ret bioconjugates are able to permeate the skin. PLP and Pyr are available as cofactors for glutamic pyruvic transaminase.Keywords: PAMAM, trans-retinal, pyridoxal phosphate, pyridoxal, transaminationFilipowicz AWołowiec SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 4819-4828 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Filipowicz A Wołowiec S Bioconjugates of PAMAM dendrimers with trans-retinal, pyridoxal, and pyridoxal phosphate
description	A Filipowicz, S WołowiecDepartment of Cosmetology, University of Information Technology and Management in Rzesz&oacute;w, Rzesz&oacute;w, PolandBackground: Bioconjugates of a polyamidoamine (PAMAM) G3 dendrimer and an aldehyde were synthesized as carriers for vitamins A and B6, and the bioavailability of these vitamins for skin nutrition was investigated.Methods: Nuclear magnetic resonance (NMR) and ultraviolet-visible methods were used to characterize the structure of the bioconjugates and for monitoring release of pyridoxal (Pyr) and pyridoxal phosphate (PLP) from these bioconjugates in vitro. A skin model permeation of bioconjugates was also studied in a Franz chamber.Results: A transdermal G3 PAMAM dendrimer was used to synthesize bioconjugates with trans-retinal (Ret), pyridoxal (Pyr), or PLP. These nanomolecules, containing up to four covalently linked Ret, Pyr, or PLP (G34Ret, G34Pyr, and G34PLP), were able to permeate the skin, as demonstrated in vitro using a model skin membrane. PLP and Pyr bound to a macromolecular vehicle were active cofactors for glutamic pyruvic transaminase, as shown by 1H NMR spectral monitoring of the progress of the L-alanine + &alpha;-ketoglutarate &rarr; glutamic acid + pyruvic acid reaction.Conclusion: PAMAM-PLP, PAMAM-Pyr, and PAMAM-Ret bioconjugates are able to permeate the skin. PLP and Pyr are available as cofactors for glutamic pyruvic transaminase.Keywords: PAMAM, trans-retinal, pyridoxal phosphate, pyridoxal, transamination
format	article
author	Filipowicz A Wołowiec S
author_facet	Filipowicz A Wołowiec S
author_sort	Filipowicz A
title	Bioconjugates of PAMAM dendrimers with trans-retinal, pyridoxal, and pyridoxal phosphate
title_short	Bioconjugates of PAMAM dendrimers with trans-retinal, pyridoxal, and pyridoxal phosphate
title_full	Bioconjugates of PAMAM dendrimers with trans-retinal, pyridoxal, and pyridoxal phosphate
title_fullStr	Bioconjugates of PAMAM dendrimers with trans-retinal, pyridoxal, and pyridoxal phosphate
title_full_unstemmed	Bioconjugates of PAMAM dendrimers with trans-retinal, pyridoxal, and pyridoxal phosphate
title_sort	bioconjugates of pamam dendrimers with trans-retinal, pyridoxal, and pyridoxal phosphate
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/cb6fff3636924f199b788698ae53b7f7
work_keys_str_mv	AT filipowicza bioconjugatesofpamamdendrimerswithtransretinalpyridoxalandpyridoxalphosphate AT wołowiecs bioconjugatesofpamamdendrimerswithtransretinalpyridoxalandpyridoxalphosphate
_version_	1718398434932686848

Bioconjugates of PAMAM dendrimers with trans-retinal, pyridoxal, and pyridoxal phosphate

Ejemplares similares