Rapid Identification of Enterococcus Faecalis Isolates by Polymerase Chain Reaction

BACKGROUND AND OBJECTIVE: Rapid detection of Enterococcus faecalis as a frequent cause of nosocomial infections in immunocompromised patients is an important issue. Herein, the current study developed a PCR assay based on the ef0737 gene to detect E. faecalis isolates. METHODS: In this cross-section...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	S Sabzi, S Ohadian Moghaddam, Z Pakbaz, MR Pourmand
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2019
Materias:	enterococcus faecalis ef073 pcr Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/cb74a9958e4447ed9aca8dcd237ad5cc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cb74a9958e4447ed9aca8dcd237ad5cc
record_format	dspace
spelling	oai:doaj.org-article:cb74a9958e4447ed9aca8dcd237ad5cc2021-11-10T08:30:36ZRapid Identification of Enterococcus Faecalis Isolates by Polymerase Chain Reaction1561-41072251-7170https://doaj.org/article/cb74a9958e4447ed9aca8dcd237ad5cc2019-12-01T00:00:00Zhttp://jbums.org/article-1-7879-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Rapid detection of Enterococcus faecalis as a frequent cause of nosocomial infections in immunocompromised patients is an important issue. Herein, the current study developed a PCR assay based on the ef0737 gene to detect E. faecalis isolates. METHODS: In this cross-sectional study, 150 clinical isolates including E. faecalis, E. faecium and Staphylococcus aureus were collected. A set of pair primers was designed using the sequence of ef0737. All isolates were examined for the presence of ef0737 gene by PCR assay. The sensitivity of PCR assay was evaluated according to 50 clinical isolates of E. faecalis. The specificity of PCR primers was also determined using non-E. faecalis species including 50 E. faecium and 50 S. aureus isolates. FINDINGS: In this study, from 150 clinical isolates that were collected; all the 50 E. faecalis isolates showed positive results for the ef0737 gene which showed 100% sensitivity. No ampliﬁcation were observed in other isolates include E. faecium and S. aureus. CONCLUSION: PCR assay is a more efficient and sensitive tool for detection and characterization of E. faecalis especially in patients with the critical condition. Identification of the preserved ef0737 gene in clinical samples may be able to determine infections caused by E. faecalis.S SabziS Ohadian MoghaddamZ PakbazMR PourmandBabol University of Medical Sciencesarticleenterococcus faecalisef073pcrMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 21, Iss 1, Pp 105-110 (2019)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	enterococcus faecalis ef073 pcr Medicine R Medicine (General) R5-920
spellingShingle	enterococcus faecalis ef073 pcr Medicine R Medicine (General) R5-920 S Sabzi S Ohadian Moghaddam Z Pakbaz MR Pourmand Rapid Identification of Enterococcus Faecalis Isolates by Polymerase Chain Reaction
description	BACKGROUND AND OBJECTIVE: Rapid detection of Enterococcus faecalis as a frequent cause of nosocomial infections in immunocompromised patients is an important issue. Herein, the current study developed a PCR assay based on the ef0737 gene to detect E. faecalis isolates. METHODS: In this cross-sectional study, 150 clinical isolates including E. faecalis, E. faecium and Staphylococcus aureus were collected. A set of pair primers was designed using the sequence of ef0737. All isolates were examined for the presence of ef0737 gene by PCR assay. The sensitivity of PCR assay was evaluated according to 50 clinical isolates of E. faecalis. The specificity of PCR primers was also determined using non-E. faecalis species including 50 E. faecium and 50 S. aureus isolates. FINDINGS: In this study, from 150 clinical isolates that were collected; all the 50 E. faecalis isolates showed positive results for the ef0737 gene which showed 100% sensitivity. No ampliﬁcation were observed in other isolates include E. faecium and S. aureus. CONCLUSION: PCR assay is a more efficient and sensitive tool for detection and characterization of E. faecalis especially in patients with the critical condition. Identification of the preserved ef0737 gene in clinical samples may be able to determine infections caused by E. faecalis.
format	article
author	S Sabzi S Ohadian Moghaddam Z Pakbaz MR Pourmand
author_facet	S Sabzi S Ohadian Moghaddam Z Pakbaz MR Pourmand
author_sort	S Sabzi
title	Rapid Identification of Enterococcus Faecalis Isolates by Polymerase Chain Reaction
title_short	Rapid Identification of Enterococcus Faecalis Isolates by Polymerase Chain Reaction
title_full	Rapid Identification of Enterococcus Faecalis Isolates by Polymerase Chain Reaction
title_fullStr	Rapid Identification of Enterococcus Faecalis Isolates by Polymerase Chain Reaction
title_full_unstemmed	Rapid Identification of Enterococcus Faecalis Isolates by Polymerase Chain Reaction
title_sort	rapid identification of enterococcus faecalis isolates by polymerase chain reaction
publisher	Babol University of Medical Sciences
publishDate	2019
url	https://doaj.org/article/cb74a9958e4447ed9aca8dcd237ad5cc
work_keys_str_mv	AT ssabzi rapididentificationofenterococcusfaecalisisolatesbypolymerasechainreaction AT sohadianmoghaddam rapididentificationofenterococcusfaecalisisolatesbypolymerasechainreaction AT zpakbaz rapididentificationofenterococcusfaecalisisolatesbypolymerasechainreaction AT mrpourmand rapididentificationofenterococcusfaecalisisolatesbypolymerasechainreaction
_version_	1718440418538946560

Rapid Identification of Enterococcus Faecalis Isolates by Polymerase Chain Reaction

Ejemplares similares