Combination of High-density Microelectrode Array and Patch Clamp Recordings to Enable Studies of Multisynaptic Integration

Abstract We present a novel, all-electric approach to record and to precisely control the activity of tens of individual presynaptic neurons. The method allows for parallel mapping of the efficacy of multiple synapses and of the resulting dynamics of postsynaptic neurons in a cortical culture. For t...

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Autores principales:	David Jäckel, Douglas J. Bakkum, Thomas L. Russell, Jan Müller, Milos Radivojevic, Urs Frey, Felix Franke, Andreas Hierlemann
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb74d1ec5ef849b4b44216b7f76001c8
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spelling	oai:doaj.org-article:cb74d1ec5ef849b4b44216b7f76001c82021-12-02T15:05:35ZCombination of High-density Microelectrode Array and Patch Clamp Recordings to Enable Studies of Multisynaptic Integration10.1038/s41598-017-00981-42045-2322https://doaj.org/article/cb74d1ec5ef849b4b44216b7f76001c82017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00981-4https://doaj.org/toc/2045-2322Abstract We present a novel, all-electric approach to record and to precisely control the activity of tens of individual presynaptic neurons. The method allows for parallel mapping of the efficacy of multiple synapses and of the resulting dynamics of postsynaptic neurons in a cortical culture. For the measurements, we combine an extracellular high-density microelectrode array, featuring 11’000 electrodes for extracellular recording and stimulation, with intracellular patch-clamp recording. We are able to identify the contributions of individual presynaptic neurons - including inhibitory and excitatory synaptic inputs - to postsynaptic potentials, which enables us to study dendritic integration. Since the electrical stimuli can be controlled at microsecond resolution, our method enables to evoke action potentials at tens of presynaptic cells in precisely orchestrated sequences of high reliability and minimum jitter. We demonstrate the potential of this method by evoking short- and long-term synaptic plasticity through manipulation of multiple synaptic inputs to a specific neuron.David JäckelDouglas J. BakkumThomas L. RussellJan MüllerMilos RadivojevicUrs FreyFelix FrankeAndreas HierlemannNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q David Jäckel Douglas J. Bakkum Thomas L. Russell Jan Müller Milos Radivojevic Urs Frey Felix Franke Andreas Hierlemann Combination of High-density Microelectrode Array and Patch Clamp Recordings to Enable Studies of Multisynaptic Integration
description	Abstract We present a novel, all-electric approach to record and to precisely control the activity of tens of individual presynaptic neurons. The method allows for parallel mapping of the efficacy of multiple synapses and of the resulting dynamics of postsynaptic neurons in a cortical culture. For the measurements, we combine an extracellular high-density microelectrode array, featuring 11’000 electrodes for extracellular recording and stimulation, with intracellular patch-clamp recording. We are able to identify the contributions of individual presynaptic neurons - including inhibitory and excitatory synaptic inputs - to postsynaptic potentials, which enables us to study dendritic integration. Since the electrical stimuli can be controlled at microsecond resolution, our method enables to evoke action potentials at tens of presynaptic cells in precisely orchestrated sequences of high reliability and minimum jitter. We demonstrate the potential of this method by evoking short- and long-term synaptic plasticity through manipulation of multiple synaptic inputs to a specific neuron.
format	article
author	David Jäckel Douglas J. Bakkum Thomas L. Russell Jan Müller Milos Radivojevic Urs Frey Felix Franke Andreas Hierlemann
author_facet	David Jäckel Douglas J. Bakkum Thomas L. Russell Jan Müller Milos Radivojevic Urs Frey Felix Franke Andreas Hierlemann
author_sort	David Jäckel
title	Combination of High-density Microelectrode Array and Patch Clamp Recordings to Enable Studies of Multisynaptic Integration
title_short	Combination of High-density Microelectrode Array and Patch Clamp Recordings to Enable Studies of Multisynaptic Integration
title_full	Combination of High-density Microelectrode Array and Patch Clamp Recordings to Enable Studies of Multisynaptic Integration
title_fullStr	Combination of High-density Microelectrode Array and Patch Clamp Recordings to Enable Studies of Multisynaptic Integration
title_full_unstemmed	Combination of High-density Microelectrode Array and Patch Clamp Recordings to Enable Studies of Multisynaptic Integration
title_sort	combination of high-density microelectrode array and patch clamp recordings to enable studies of multisynaptic integration
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cb74d1ec5ef849b4b44216b7f76001c8
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Combination of High-density Microelectrode Array and Patch Clamp Recordings to Enable Studies of Multisynaptic Integration

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