Dermal fibroblast-like cells reprogrammed directly from adipocytes in mouse

Abstract In deep burns, early wound closure is important for healing, and skin grafting is mainly used for wound closure. However, it is difficult to achieve early wound closure in extensive total body surface area deep burns due to the lack of donor sites. Dermal fibroblasts, responsible for dermis...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mitsunobu Toyosaki, Koichiro Homma, Sayuri Suzuki, Naoto Muraoka, Hisayuki Hashimoto, Naoki Goshima, Masaki Ieda, Junichi Sasaki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cb7afdbab3fc4e1aac051218ec7a598c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cb7afdbab3fc4e1aac051218ec7a598c
record_format	dspace
spelling	oai:doaj.org-article:cb7afdbab3fc4e1aac051218ec7a598c2021-12-02T11:43:58ZDermal fibroblast-like cells reprogrammed directly from adipocytes in mouse10.1038/s41598-020-78523-82045-2322https://doaj.org/article/cb7afdbab3fc4e1aac051218ec7a598c2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78523-8https://doaj.org/toc/2045-2322Abstract In deep burns, early wound closure is important for healing, and skin grafting is mainly used for wound closure. However, it is difficult to achieve early wound closure in extensive total body surface area deep burns due to the lack of donor sites. Dermal fibroblasts, responsible for dermis formation, may be lost in deep burns. However, fat layers composed of adipocytes, lying underneath the dermis, are retained even in such cases. Direct reprogramming is a novel method for directly reprograming some cells into other types by introducing specific master regulators; it has exhibited appreciable success in various fields. In this study, we aimed to assess whether the transfection of master regulators (ELF4, FOXC2, FOXO1, IRF1, PRRX1, and ZEB1) could reprogram mouse adipocytes into dermal fibroblast-like cells. Our results indicated the shrinkage of fat droplets in reprogrammed mouse adipocytes and their transformation into spindle-shaped dermal fibroblasts. Reduced expression of PPAR-2, c/EBP, aP2, and leptin, the known markers of adipocytes, in RT-PCR, and enhanced expression of anti-ER-TR7, the known anti-fibroblast marker, in immunocytochemistry, were confirmed in the reprogrammed mouse adipocytes. The dermal fibroblast-like cells, reported here, may open up a new treatment mode for enabling early closure of deep burn wounds.Mitsunobu ToyosakiKoichiro HommaSayuri SuzukiNaoto MuraokaHisayuki HashimotoNaoki GoshimaMasaki IedaJunichi SasakiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mitsunobu Toyosaki Koichiro Homma Sayuri Suzuki Naoto Muraoka Hisayuki Hashimoto Naoki Goshima Masaki Ieda Junichi Sasaki Dermal fibroblast-like cells reprogrammed directly from adipocytes in mouse
description	Abstract In deep burns, early wound closure is important for healing, and skin grafting is mainly used for wound closure. However, it is difficult to achieve early wound closure in extensive total body surface area deep burns due to the lack of donor sites. Dermal fibroblasts, responsible for dermis formation, may be lost in deep burns. However, fat layers composed of adipocytes, lying underneath the dermis, are retained even in such cases. Direct reprogramming is a novel method for directly reprograming some cells into other types by introducing specific master regulators; it has exhibited appreciable success in various fields. In this study, we aimed to assess whether the transfection of master regulators (ELF4, FOXC2, FOXO1, IRF1, PRRX1, and ZEB1) could reprogram mouse adipocytes into dermal fibroblast-like cells. Our results indicated the shrinkage of fat droplets in reprogrammed mouse adipocytes and their transformation into spindle-shaped dermal fibroblasts. Reduced expression of PPAR-2, c/EBP, aP2, and leptin, the known markers of adipocytes, in RT-PCR, and enhanced expression of anti-ER-TR7, the known anti-fibroblast marker, in immunocytochemistry, were confirmed in the reprogrammed mouse adipocytes. The dermal fibroblast-like cells, reported here, may open up a new treatment mode for enabling early closure of deep burn wounds.
format	article
author	Mitsunobu Toyosaki Koichiro Homma Sayuri Suzuki Naoto Muraoka Hisayuki Hashimoto Naoki Goshima Masaki Ieda Junichi Sasaki
author_facet	Mitsunobu Toyosaki Koichiro Homma Sayuri Suzuki Naoto Muraoka Hisayuki Hashimoto Naoki Goshima Masaki Ieda Junichi Sasaki
author_sort	Mitsunobu Toyosaki
title	Dermal fibroblast-like cells reprogrammed directly from adipocytes in mouse
title_short	Dermal fibroblast-like cells reprogrammed directly from adipocytes in mouse
title_full	Dermal fibroblast-like cells reprogrammed directly from adipocytes in mouse
title_fullStr	Dermal fibroblast-like cells reprogrammed directly from adipocytes in mouse
title_full_unstemmed	Dermal fibroblast-like cells reprogrammed directly from adipocytes in mouse
title_sort	dermal fibroblast-like cells reprogrammed directly from adipocytes in mouse
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/cb7afdbab3fc4e1aac051218ec7a598c
work_keys_str_mv	AT mitsunobutoyosaki dermalfibroblastlikecellsreprogrammeddirectlyfromadipocytesinmouse AT koichirohomma dermalfibroblastlikecellsreprogrammeddirectlyfromadipocytesinmouse AT sayurisuzuki dermalfibroblastlikecellsreprogrammeddirectlyfromadipocytesinmouse AT naotomuraoka dermalfibroblastlikecellsreprogrammeddirectlyfromadipocytesinmouse AT hisayukihashimoto dermalfibroblastlikecellsreprogrammeddirectlyfromadipocytesinmouse AT naokigoshima dermalfibroblastlikecellsreprogrammeddirectlyfromadipocytesinmouse AT masakiieda dermalfibroblastlikecellsreprogrammeddirectlyfromadipocytesinmouse AT junichisasaki dermalfibroblastlikecellsreprogrammeddirectlyfromadipocytesinmouse
_version_	1718395308921061376

Dermal fibroblast-like cells reprogrammed directly from adipocytes in mouse

Ejemplares similares